Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein.

abstract：:A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...

journal_title：Plant molecular biology

authors： Nakajima T,Suzůki A,Tanifuji S,Kato A

更新日期：1992-12-01 00:00:00

abstract：:We have sequenced a plastid gene cluster from the unicellular red alga Cyanidium caldarium which is located downstream from the psbA gene and contains, in the following order, genes for a beta-allophycocyanin-like protein (apcB'), a putative 9.5 kDa allophycocyanin linker protein (apcL9.5) and a putative 29 kDa phycoc...

journal_title：Plant molecular biology

authors： Valentin K,Maid U,Emich A,Zetsche K

更新日期：1992-10-01 00:00:00

abstract：:Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...

journal_title：Plant molecular biology

authors： Pareek A,Singla SL,Grover A

更新日期：1995-10-01 00:00:00

abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...

journal_title：Plant molecular biology

authors： Li YZ,Pan YH,Sun CB,Dong HT,Luo XL,Wang ZQ,Tang JL,Chen B

更新日期：2010-12-01 00:00:00

abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

journal_title：Plant molecular biology

authors： Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

更新日期：1998-02-01 00:00:00

abstract：:The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...

journal_title：Plant molecular biology

authors： Granger CL,Callos JD,Medford JI

更新日期：1996-05-01 00:00:00

abstract：:Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

journal_title：Plant molecular biology

authors： Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

更新日期：2011-11-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

journal_title：Plant molecular biology

authors： Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

更新日期：1997-10-01 00:00:00

abstract：:We previously identified a cluster of d ormancy-a ssociated M ADS-box transcription factors (DAM genes) in peach [Prunus persica (L.) Batsch] as potential candidates for control of the non-dormant phenotype observed in the evg mutant. Of these genes, DAM3, DAM5 and DAM6 were winter expressed, suggesting a role for the...

journal_title：Plant molecular biology

authors： Jiménez S,Reighard GL,Bielenberg DG

更新日期：2010-05-01 00:00:00

abstract：:Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...

journal_title：Plant molecular biology

authors： Liu C,Muchhal US,Raghothama KG

更新日期：1997-03-01 00:00:00

abstract：:The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...

journal_title：Plant molecular biology

authors： Oung HM,Lin KC,Wu TM,Chandrika NN,Hong CY

更新日期：2015-12-01 00:00:00

abstract：:The entire mitochondrial genome (mt genome) of the unicellular green alga Platymonas subcordiformis (synonym Tetraselmis subcordiformis; Prasinophyceae) was cloned and a physical map for the four restriction enzymes Hind III, Eco RI, Bg/II and Xba I was constructed. The mt genome of P. subcordiformis is a 42.8 kb circ...

journal_title：Plant molecular biology

authors： Kessler U,Zetsche K

更新日期：1995-12-01 00:00:00

abstract：:Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...

journal_title：Plant molecular biology

authors： Prassinos C,Haralampidis K,Milioni D,Samakovli D,Krambis K,Hatzopoulos P

更新日期：2008-07-01 00:00:00

abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

journal_title：Plant molecular biology

authors： Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

更新日期：2019-03-01 00:00:00

abstract：:Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...

journal_title：Plant molecular biology

authors： Majerová E,Fojtová M,Mozgová I,Bittová M,Fajkus J

更新日期：2011-11-01 00:00:00

abstract：:Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...

journal_title：Plant molecular biology

authors： Dunlevy JD,Soole KL,Perkins MV,Dennis EG,Keyzers RA,Kalua CM,Boss PK

更新日期：2010-09-01 00:00:00

abstract：:Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated wi...

journal_title：Plant molecular biology

authors： Pankievicz VC,Camilios-Neto D,Bonato P,Balsanelli E,Tadra-Sfeir MZ,Faoro H,Chubatsu LS,Donatti L,Wajnberg G,Passetti F,Monteiro RA,Pedrosa FO,Souza EM

更新日期：2016-04-01 00:00:00

abstract：KEY MESSAGE:Modification of the poplar defense pathway through pathogen-induced expression of an amphibian host defense peptide modulates plant innate immunity and confers robust and reliable resistance against a major poplar pathogen, Septoria musiva. Host defense peptides (HDPs), also known as cationic antimicrobial ...

journal_title：Plant molecular biology

authors： Yevtushenko DP,Misra S

更新日期：2019-07-01 00:00:00

abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

journal_title：Plant molecular biology

authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

更新日期：1997-05-01 00:00:00

abstract：:A mutant of alfalfa mosaic virus (AMV), which in contrast to wild type (wt) can invade cowpea plants systemically, is replicated more efficiently in cowpea protoplasts than the wt. Mutant preparations isolated from infected cowpea protoplasts contained a higher amount of middle component (M, containing RNA 2) than wt ...

journal_title：Plant molecular biology

authors： Roosien J,Sarachu AN,Alblas F,Van Vloten-Doting L

更新日期：1983-03-01 00:00:00

abstract：:We have characterized a genomic clone containing the potato pathogenesis-related genes STH-2 and STH-21. The two genes are found 4 kb apart on the same chromosome and their sequences are highly similar. They present the same transcriptional orientation and are both interrupted by a single intron. A chimaeric gene cons...

journal_title：Plant molecular biology

authors： Matton DP,Prescott G,Bertrand C,Camirand A,Brisson N

更新日期：1993-05-01 00:00:00

abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...

journal_title：Plant molecular biology

authors： Müller BS,Sakamoto T,de Menezes IP,Prado GS,Martins WS,Brondani C,de Barros EG,Vianello RP

更新日期：2014-11-01 00:00:00

abstract：:Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...

journal_title：Plant molecular biology

authors： Wu J,Gu YQ,Hu Y,You FM,Dandekar AM,Leslie CA,Aradhya M,Dvorak J,Luo MC

更新日期：2012-01-01 00:00:00

abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

journal_title：Plant molecular biology

authors： Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

更新日期：1991-10-01 00:00:00

abstract：:Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...

journal_title：Plant molecular biology

authors： Gothandam KM,Nalini E,Karthikeyan S,Shin JS

更新日期：2010-01-01 00:00:00

abstract：:Cotton fiber elongation, largely achieved by cell wall loosening, is an important stage during cotton fiber development. In this present research, a fiber preferential cDNA encoding a pectate lyase (PEL) which could exclusively degrade the de-esterified pectin was isolated from a cotton (Gossypium hirsutum) fiber cDNA...

journal_title：Plant molecular biology

authors： Wang H,Guo Y,Lv F,Zhu H,Wu S,Jiang Y,Li F,Zhou B,Guo W,Zhang T

更新日期：2010-03-01 00:00:00

abstract：:Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

journal_title：Plant molecular biology

authors： Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

更新日期：1995-09-01 00:00:00

abstract：:A new cytoplasmic male sterile sunflower, CMS3 [44], was characterised in relation to the Petiolaris (PET1) cytoplasmic male-sterile sunflower, CMS89 [25]. Southern blot analysis showed that the mitochondrial genome of CMS3 contains unique rearrangements in at least five loci (atp6, atp9, atpA, nad1 + 5 and coxIII) co...

journal_title：Plant molecular biology

authors： Spassova M,Moneger F,Leaver CJ,Petrov P,Atanassov A,Nijkamp HJ,Hille J

更新日期：1994-12-01 00:00:00

abstract：:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

journal_title：Plant molecular biology

authors： Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

更新日期：2007-02-01 00:00:00