Abstract:
:Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Majerová E,Fojtová M,Mozgová I,Bittová M,Fajkus Jdoi
10.1007/s11103-011-9816-7subject
Has Abstractpub_date
2011-11-01 00:00:00pages
371-80issue
4-5eissn
0167-4412issn
1573-5028journal_volume
77pub_type
杂志文章abstract:KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01037-4
更新日期:2020-09-01 00:00:00
abstract::In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4922-z
更新日期:2006-03-01 00:00:00
abstract::DNA methylation plays an important role in the gene-silencing network of higher eukaryotes. We have analyzed the 21.5-kb maintenance methyltransferase (M-MTase) gene, met1, of the multicellular green alga Volvox carteri. The met1 transcript was detected only during the period when DNA replication and cell division are...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9091-1
更新日期:2007-02-01 00:00:00
abstract::Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9229-9
更新日期:2007-11-01 00:00:00
abstract::In yeast and mammals, prohibitins (PHBs) are considered as structural proteins that form a scaffold-like structure for interacting with a set of proteins involved in various processes occurring in the mitochondria. The role of PHB in plant mitochondria is poorly understood. In the study, the model organism Arabidopsis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0320-3
更新日期:2015-06-01 00:00:00
abstract::We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017817
更新日期:1996-01-01 00:00:00
abstract::In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0246-1
更新日期:2014-11-01 00:00:00
abstract::Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023013
更新日期:1981-03-01 00:00:00
abstract::Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015670
更新日期:1988-03-01 00:00:00
abstract::TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1020719420867
更新日期:2003-01-01 00:00:00
abstract::Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00038998
更新日期:1993-04-01 00:00:00
abstract::The single-copy gene encoding the Arabidopsis HMG-I/Y protein was isolated and characterised. The gene encodes a protein of 204 amino acid residues and contains a single intron of 73 bp. Primer extension analysis indicates that transcription starts 115 bp upstream of the translation start and the leader sequence conta...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005928219895
更新日期:1998-04-01 00:00:00
abstract::A gene family of at least five members encodes the tobacco mitochondrial Rieske Fe-S protein (RISP). To determine whether all five RISPs are translocated to mitochondria, fusion proteins containing the putative presequences of tobacco RISPs and Escherichia coli beta-glucuronidase (GUS) were expressed in transgenic tob...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020982
更新日期:1995-11-01 00:00:00
abstract::We have previously isolated a Brassica juncea cDNA encoding BjCHI1, a novel chitinase with two chitin-binding domains, and have shown that its mRNA is induced by wounding and methyl jasmonate treatment (K.-J. Zhao and M.-L. Chye, Plant Mol. Biol. 40 (1999) 1009-1018). By the presence of two chitin-binding domains, BjC...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016067200148
更新日期:2002-09-01 00:00:00
abstract::Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00016474
更新日期:1994-12-01 00:00:00
abstract::Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029606
更新日期:1994-07-01 00:00:00
abstract::The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00981-5
更新日期:2020-05-01 00:00:00
abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019520
更新日期:1988-11-01 00:00:00
abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0555-7
更新日期:2017-02-01 00:00:00
abstract::Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9722-4
更新日期:2011-02-01 00:00:00
abstract::Hazelnut (Corylus avellana L.) is a species of economic interest that shows a peculiar floral biology. Unlike most of the angiosperms, which produce ovules during floral development such that they are ready for pollen at anthesis, hazelnut ovary development is delayed and triggered by compatible pollination. In order ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006022524708
更新日期:1998-12-01 00:00:00
abstract::We have isolated full-size cDNA sequences encoding the photosynthetic isoform of pyruvate orthophosphate dikinase (PPDK) of the C3 plant Flaveria pringlei. The encoded protein shares 96% identical amino acid residues with the C4 isoform of PPDK in the C4 species F. trinervia. The differing amino acid residues are even...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00013761
更新日期:1994-10-01 00:00:00
abstract::The first step in the routing of newly synthesized proteins into the secretory pathway is the binding of the nascent signal sequence to the signal recognition particle. The mammalian signal recognition particle is a complex consisting of 6 proteins and a single 7S RNA molecule. Signal recognition particle-like complex...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042359
更新日期:1993-12-01 00:00:00
abstract::We have characterized a gene specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis. Differential screening led to the isolation of a floral nectary-specific cDNA clone. Northern hybridization indicated that its mRNA transcript is 1450 nucleotides long and specific to the flower base....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006381625421
更新日期:2000-03-01 00:00:00
abstract::Arabidopsis is currently the reference genome for higher plants. A new, more detailed statistical analysis of Arabidopsis gene structure is presented including intron and exon lengths, intergenic distances, features of promoters, and variant 5'-ends of mRNAs transcribed from the same transcription unit. We also provid...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2564-9
更新日期:2006-01-01 00:00:00
abstract::To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9780-2
更新日期:2011-05-01 00:00:00
abstract::In this paper we report on the organization and expression of the two chalcone flavanone isomerase (CHI) genes A and B from thePetunia hybrida inbred line V30. From a combination of sequence data, primer extension and RNAse protection experiments we infer the presence of two promoters PA1 and PA2 upstream of the CHI g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036968
更新日期:1989-05-01 00:00:00
abstract::Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7507-3
更新日期:2005-09-01 00:00:00
abstract::Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of NH3 with glutamate to produce glutamine. In plants GS is an octameric enzyme and is located either in the cytoplasm (GS1) or in the chloroplast (GS2). Two distinct classes of GS1 genes with unique 3'-untranslated region (3'UTR) have been identified ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006099512706
更新日期:1998-06-01 00:00:00
abstract::The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0240-7
更新日期:2014-11-01 00:00:00