Abstract:
:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-dimensional gel electrophoresis. Silver staining revealed approximately 40 PBS polypeptides. Ex planta (35)S-methionine labeling of PBM-enclosed bacteroids revealed that about 90% of the PBS proteins are synthesized by the bacteroid. Approximately 30% of the PBS polypeptides are common between the PBS and the periplasmic space of free-living bacteria; one (38kDa) PBS protein is also excreted by free-living bacteria in the bacterial culture medium. At least four bacteroid-encoded PBS polypeptides were clearly identified as symbiosis-specific.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RCdoi
10.1007/BF00015670subject
Has Abstractpub_date
1988-03-01 00:00:00pages
183-90issue
2eissn
0167-4412issn
1573-5028journal_volume
11pub_type
杂志文章abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00040537
更新日期:1992-09-01 00:00:00
abstract:KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01002-1
更新日期:2020-07-01 00:00:00
abstract::Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9041-y
更新日期:2006-11-01 00:00:00
abstract::Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000028775.58537.fe
更新日期:2004-01-01 00:00:00
abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020254
更新日期:1995-05-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00
abstract::Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9092-0
更新日期:2007-02-01 00:00:00
abstract::Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006279720960
更新日期:1999-08-01 00:00:00
abstract::The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0240-7
更新日期:2014-11-01 00:00:00
abstract::Nonsense-mediated mRNA decay (NMD) is a eukaryotic quality control system that identifies and eliminates transcripts having a premature translation termination codon (PTC). NMD is also involved in the control of several wild-type mRNAs. The NMD core machinery consists of three highly conserved NMD factors (UPF1, UPF2 ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9726-0
更新日期:2011-02-01 00:00:00
abstract::We have identified by differential screening a novel Arabidopsis thaliana gene, called kin1, which is induced at +4 degrees C. The nucleotide sequences of both the genomic clone and the corresponding cDNA were determined. The deduced 6.5 kDa polypeptide has an unusual amino acid composition being rich in alanine, glyc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017731
更新日期:1990-07-01 00:00:00
abstract::Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037068
更新日期:1991-10-01 00:00:00
abstract::Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8519-3
更新日期:2005-09-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract::The expression of the alpha' and beta subunit genes of beta-conglycinin is differentially regulated during soybean embryo development. Although both are expressed solely in developing seeds during mid to late stages of embryo development, the alpha' subunit is expressed more highly on a per gene basis, and alpha' subu...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023991
更新日期:1991-03-01 00:00:00
abstract:KEY MESSAGE:AcGST1, an anthocyanin-related GST, may functions as a carrier to transport anthocyanins from ER to tonoplast in kiwifruit. It was positively regulated by AcMYBF110 through directly binding to its promoter. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum but accumulate p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00870-6
更新日期:2019-07-01 00:00:00
abstract::The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9494-x
更新日期:2009-07-01 00:00:00
abstract::Two soybean ribulose-1,5-bisphosphate carboxylase small subunit (SSU) genes, SRS1 and SRS4, are highly homologous over a region that includes 4 kb of 5' and 1 kb of 3' flanking sequences. The expression of these genes was compared using synthetic oligonucleotide probes. Analysis of a soybean leaf cDNA library indicate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019389
更新日期:1990-06-01 00:00:00
abstract:KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00890-2
更新日期:2019-09-01 00:00:00
abstract::The folding and assembly of proteins in cells often requires the assistance of molecular chaperones such as the Hsp70 and Hsp60 heat shock proteins. Hsp70 chaperones cooperate with DnaJ and GrpE homologues to ensure a productive folding cycle. In this study we describe the gene for the first chloroplast localized DnaJ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005784115363
更新日期:1997-01-01 00:00:00
abstract::The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006493021557
更新日期:2001-01-01 00:00:00
abstract::Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028854
更新日期:1994-11-01 00:00:00
abstract::The caffeine biosynthetic pathway in coffee plants has been proposed to involve three distinct N -methyltransferases, xanthosine methyltransferase (XMT), 7- N -methylxanthine methyltransferase (MXMT; theobromine synthase), and 3,7-dimethylxanthine methyltransferase (DXMT; caffeine synthase). We previously isolated all...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-0393-x
更新日期:2004-04-01 00:00:00
abstract::Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0460-0
更新日期:2016-06-01 00:00:00
abstract::We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9284-2
更新日期:2008-03-01 00:00:00
abstract::The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1015500431421
更新日期:2002-08-01 00:00:00
abstract::Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interact...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9639-y
更新日期:2010-07-01 00:00:00
abstract::Selective gene expression in different populations of cells of the root apex of transgenic tobacco could be evidenced by means of GUS constructs with deletions of the rolB promoter and fusions with the CaMV 35S minimal promoter. Five regulatory regions have been broadly identified in the rolB 5' non-coding region. The...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029606
更新日期:1994-07-01 00:00:00
abstract::The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we dev...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9379-4
更新日期:2008-11-01 00:00:00