Bacteroid-encoded proteins are secreted into the peribacteroid space by Rhizobium leguminosarum.

Abstract:

:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-dimensional gel electrophoresis. Silver staining revealed approximately 40 PBS polypeptides. Ex planta (35)S-methionine labeling of PBM-enclosed bacteroids revealed that about 90% of the PBS proteins are synthesized by the bacteroid. Approximately 30% of the PBS polypeptides are common between the PBS and the periplasmic space of free-living bacteria; one (38kDa) PBS protein is also excreted by free-living bacteria in the bacterial culture medium. At least four bacteroid-encoded PBS polypeptides were clearly identified as symbiosis-specific.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

doi

10.1007/BF00015670

subject

Has Abstract

pub_date

1988-03-01 00:00:00

pages

183-90

issue

2

eissn

0167-4412

issn

1573-5028

journal_volume

11

pub_type

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