Abstract：
:Nicotiana tabacum Togt encodes a scopoletin glucosyltransferase (UDPglucose:scopoletin O -beta-D-glucosyltrans- ferase, EC 2.4.1.128) known to act in vitro on many different substrates including the 6-methoxy-7-hydroxy- coumarin scopoletin. This phenolic compound accumulates in vast amounts, essentially in its glucosylated form scopolin, in tobacco during the hypersensitive response (HR) to tobacco mosaic virus (TMV). To identify the physiological role of this pathogen-inducible UDP-Glc glucosyltransferase (UGT), we generated TOGT over-expressing transgenic plants. Although no endogenous scopoletin or scopolin could be detected before infection, the accumulation of both the aglycone and the glucoside was found to be 2-fold higher in transgenic plants after inoculation with TMV than in wild-type plants. Scopoletin UGT activity in plants over-expressing Togt was significantly higher during the HR than in control plants. This up-regulated activity was associated with a strong increase of the bright blue fluorescence surrounding the HR-necrotic lesions under UV light, which is known to correlate with scopoletin and scopolin abundance. Necrosis appeared sooner in transgenic plants and lesions developed faster, suggesting an accelerated HR. Unexpectedly, the viral content in each lesion was not significantly different in transgenic and in wild-type plants. These results are discussed in relation to the role of TOGT as the major UDP-Glc: scopoletin glucosyltransferase and to the importance of scopoletin accumulation during the HR.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Gachon C,Baltz R,Saindrenan Pdoi
10.1023/B:PLAN.0000028775.58537.fesubject
Has Abstractpub_date
2004-01-01 00:00:00pages
137-46issue
1eissn
0167-4412issn
1573-5028pii
5267902journal_volume
54pub_type
杂志文章abstract：:The molecular mechanism of signal transduction pathways which mediate the action of phytohormones are poorly understood. Recently, we and others have shown that the as -1 type cis-acting elements can respond to auxin and salicylic acid, two well-characterized signaling molecules in plants. In the present work, we have...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019093
更新日期：1996-11-01 00:00:00
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journal_title：Plant molecular biology
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更新日期：1992-05-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015650
更新日期：1990-01-01 00:00:00
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doi：10.1007/s11103-015-0317-y
更新日期：2015-06-01 00:00:00
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更新日期：2012-02-01 00:00:00
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doi：10.1007/s11103-015-0380-4
更新日期：2015-12-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017921
更新日期：1991-01-01 00:00:00
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journal_title：Plant molecular biology
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更新日期：2001-10-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
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更新日期：2003-08-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
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更新日期：1998-08-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9009-y
更新日期：2006-09-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016069
更新日期：1991-06-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0445-z
更新日期：2016-05-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0575-3
更新日期：2017-03-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9816-7
更新日期：2011-11-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00036916
更新日期：1990-08-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：1998-09-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章,已发布勘误
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更新日期：2017-12-01 00:00:00
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journal_title：Plant molecular biology
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更新日期：2013-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11103-005-2564-9
更新日期：2006-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s11103-013-0161-x
更新日期：2014-04-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006094015513
更新日期：1998-11-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-3474-y
更新日期：2004-10-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0405-z
更新日期：2016-01-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0794-x
更新日期：2018-12-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006133311402
更新日期：1999-04-01 00:00:00
abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9441-2
更新日期：2009-03-01 00:00:00
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journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0252-3
更新日期：2014-12-01 00:00:00
