Abstract:
:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters and is bound by the tomato bZIP transcription factor VSF-1. Here, we analysed the interaction of VSF-1 with fragments of the vs-1 element and studied the molecular basis of specific binding both in the DNA sequence of the promoter element as well as in the protein. The minimal binding site of VSF-1 is a 9 bp, non-palindromic sequence with two non-identical half-sites and a central nucleotide which separates them. The amino acid sequence of the VSF-1 DNA-binding basic domain has a Lys at position -10 instead of a conserved Arg found in the other bZIP factors isolated so far. This lysine was found to be required for specific recognition of the non-palindromic binding site: a mutant VSF-1 with a Lys-to-Arg substitution at position -10 bound with higher affinity to a palindromic sequence than the wild-type protein. The minimal binding site of VSF-1 was sufficient and necessary to confer vascular-specific expression to a heterologous promoter in vivo. The vsf-1 promoter also showed vascular-specific expression in transgenic tobacco. The close similarity of these expression patterns suggests that VSF-1 is specifically involved in vascular expression of the grp1.8 gene in plants.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ringli C,Keller Bdoi
10.1023/a:1006030007333subject
Has Abstractpub_date
1998-08-01 00:00:00pages
977-88issue
6eissn
0167-4412issn
1573-5028journal_volume
37pub_type
杂志文章abstract::Heterokont algae such as diatoms, brown seaweeds and the raphidophyte Heterosigma akashiwo acquired their chloroplasts via a secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in chloroplasts surrounded by four membranes rather than two. The precursor of a nuclear-encoded thylak...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1023900100803
更新日期:2003-05-01 00:00:00
abstract:KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...
journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1986-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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更新日期:2002-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021807
更新日期:1996-05-01 00:00:00
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journal_title:Plant molecular biology
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doi:10.1023/a:1006038117130
更新日期:1998-12-01 00:00:00
abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0156-7
更新日期:2014-03-01 00:00:00
abstract::Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9849-y
更新日期:2012-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01085-w
更新日期:2020-10-21 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01045-4
更新日期:2020-10-01 00:00:00
abstract::The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0248-z
更新日期:2014-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006498413543
更新日期:2000-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026599028063
更新日期:2000-11-01 00:00:00
abstract::When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020560
更新日期:1991-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2005-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1993-03-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1990-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010637012528
更新日期:2001-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0581-5
更新日期:2017-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023557
更新日期:1994-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1025070827979
更新日期:2003-07-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:1993-10-01 00:00:00