Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts.

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00

abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...

journal_title：Plant molecular biology

authors： Wirdnam C,Motoyama A,Arn-Bouldoires E,van Eeden S,Iglesias A,Meins F Jr

更新日期：2004-11-01 00:00:00

abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...

journal_title：Plant molecular biology

authors： Katinakis P,Lankhorst RM,Louwerse J,van Kammen A,van den Bos RC

更新日期：1988-03-01 00:00:00

abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...

journal_title：Plant molecular biology

authors： Schuster J,Binder S

更新日期：2005-01-01 00:00:00

abstract：:Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have ...

journal_title：Plant molecular biology

authors： DeRocher A,Vierling E

更新日期：1995-02-01 00:00:00

abstract：:The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

journal_title：Plant molecular biology

authors： Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

更新日期：1987-01-01 00:00:00

abstract：:Calcium-dependent protein kinases (CDPKs) are crucial sensors of calcium concentration changes in plant cells under diverse endogenous and environmental stimuli. We identified 20 CDPK genes from bread wheat and performed a comprehensive study on their structural, functional and evolutionary characteristics. Full-lengt...

journal_title：Plant molecular biology

authors： Li AL,Zhu YF,Tan XM,Wang X,Wei B,Guo HZ,Zhang ZL,Chen XB,Zhao GY,Kong XY,Jia JZ,Mao L

更新日期：2008-03-01 00:00:00

abstract：KEY MESSAGE:1599 novel circRNAs and 1583 heat stress-specific circRNAs were identified in Arabidopsis. Heat stress enhanced accumulation of circRNAs remarkably. Heat stress altered the sizes of circRNAs, numbers of circularized exons and alterative circularization events. A putative circRNA-mediated ceRNA networks unde...

journal_title：Plant molecular biology

authors： Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

更新日期：2018-02-01 00:00:00

abstract：:In order to analyse the cruciferin gene family in radish a cDNA library was screened either with heterologous rapeseed probes or by differential screening and sequencing. We could identify six partial cDNA clones belonging to two different groups of cruciferin genes which do not cross-hybridize, and probably three dis...

journal_title：Plant molecular biology

authors： Depigny-This D,Raynal M,Aspart L,Delseny M,Grellet F

更新日期：1992-11-01 00:00:00

abstract：:Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...

journal_title：Plant molecular biology

authors： Zhang XF,Jiang T,Wu Z,Du SY,Yu YT,Jiang SC,Lu K,Feng XJ,Wang XF,Zhang DP

更新日期：2013-10-01 00:00:00

abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

journal_title：Plant molecular biology

authors： Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

更新日期：1991-10-01 00:00:00

abstract：:Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...

journal_title：Plant molecular biology

authors： Chye ML,Cheung KY

更新日期：1995-10-01 00:00:00

abstract：:Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...

journal_title：Plant molecular biology

authors： Duan M,Sun SS

更新日期：2005-09-01 00:00:00

abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...

journal_title：Plant molecular biology

authors： Chye ML

更新日期：1998-11-01 00:00:00

abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...

journal_title：Plant molecular biology

authors： Saint-Guily A,Schantz ML,Schantz R

更新日期：1994-03-01 00:00:00

abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

journal_title：Plant molecular biology

authors： Ni Z,Hu Z,Jiang Q,Zhang H

更新日期：2013-05-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

journal_title：Plant molecular biology

authors： Teo WL,Kumar P,Goh CJ,Swarup S

更新日期：2001-07-01 00:00:00

abstract：:A drought- and abscisic acid (ABA)-inducible gene, His1, was isolated from Lycopersicon pennellii, a drought-resistant relative of cultivated tomato, and the gene structure was defined experimentally. The nucleotide sequence of His1 predicts a protein of 202 amino acid residues, with a significant sequence homology to...

journal_title：Plant molecular biology

authors： Wei T,O'Connell MA

更新日期：1996-01-01 00:00:00

abstract：:The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....

journal_title：Plant molecular biology

authors： Imajuku Y,Ohashi Y,Aoyama T,Goto K,Oka A

更新日期：2001-05-01 00:00:00

abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...

journal_title：Plant molecular biology

authors： Sun E,Shapiro DR,Wu BW,Tewari KK

更新日期：1986-11-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...

journal_title：Plant molecular biology

authors： Su J,Yang X,He J,Zhang Y,Duan X,Wang R,Shen W

更新日期：2019-11-01 00:00:00

abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...

journal_title：Plant molecular biology

authors： Gil P,Green PJ

更新日期：1997-07-01 00:00:00

abstract：:The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...

journal_title：Plant molecular biology

authors： Cheng SH,Keller B,Condit CM

更新日期：1996-04-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...

journal_title：Plant molecular biology

authors： Al-Attala MN,Wang X,Abou-Attia MA,Duan X,Kang Z

更新日期：2014-03-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

journal_title：Plant molecular biology

authors： Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

更新日期：2003-09-01 00:00:00

abstract：:An important group of splicing factors involved in constitutive and alternative splicing contain an arginine/serine (RS)-rich domain. We have previously demonstrated the existence of such factors in plants and report now on a new family of splicing factors (termed the RSZ family) from Arabidopsis thaliana which additi...

journal_title：Plant molecular biology

authors： Lopato S,Gattoni R,Fabini G,Stevenin J,Barta A

更新日期：1999-03-01 00:00:00