Abstract:
:A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the mutant was named faceless pollen-1 (flp1). Transmission electron microscopy (TEM) revealed that the smooth appearance was due to tryphine filling in the exine cavities and covering the pollen surface. The lipid droplets in the tryphine of mutant pollen were smaller and more numerous than those of the wild type. SEM analysis also demonstrated that pollen exine was easily damaged by acetolysis, suggesting that a component of exine, sporopollenin, was defective in the mutant. In addition, the stems and siliques had reduced amounts of wax crystals. A predicted amino acid sequence of the cDNA that corresponded to the tagged gene, fip1, showed sequence similarity to proteins involved in wax biosynthesis. The FLP1 protein is likely to play a role in the synthesis of the components of tryphine, sporopollenin of exine and the wax of stems and siliques.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama Kdoi
10.1023/B:PLAN.0000009269.97773.70subject
Has Abstractpub_date
2003-09-01 00:00:00pages
107-16issue
1-2eissn
0167-4412issn
1573-5028pii
5149836journal_volume
53pub_type
杂志文章abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000019059.56489.ca
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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更新日期:1999-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006022524708
更新日期:1998-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2003-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
abstract::The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
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pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00