TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.


:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcriptional factors, the protein products of the tobacco TGA1a family are likely to form hetero-dimers with each other in addition to the homo-dimers. In order to find a model plant system that may have less genomic complexity, we have now characterized a TGA1a-related gene (TGA3) from Arabidopsis thaliana. Southern blot analyses at high stringency suggest that Arabidopsis contains only one copy of TGA3 per haploid genome. However, low stringency Southern blot analyses with homologous and heterologous probes suggest that there is a multigene family of TGA1a-related genes present in Arabidopsis, of which TGA1, TGA2 and TGA3 are members. Although these gene members share a highly conserved bZIP region, they are not genes with high homologies at the nucleotide level. Similar to TGA1a of tobacco, TGA3 is most highly expressed in root tissues and recombinant TGA3 protein shows similar DNA-binding site specificity to that of TGA1a in vitro. Comparison of the genomic organization between TGA3 and the tobacco homologue PG13 reveals striking conservation in the sizes and positions of exons and introns in the region surrounding the bZIP domain.


Plant Mol Biol


Plant molecular biology


Miao ZH,Liu X,Lam E




Has Abstract


1994-04-01 00:00:00












  • Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco.

    abstract::The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

    更新日期:1995-04-01 00:00:00

  • A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas.

    abstract::Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Luo L,Herrin DL

    更新日期:2012-07-01 00:00:00

  • Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression.

    abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van der Zaal EJ,Droog FN,Boot CJ,Hensgens LA,Hoge JH,Schilperoort RA,Libbenga KR

    更新日期:1991-06-01 00:00:00

  • The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants.

    abstract::Many studies in both animal and plant systems have shown that matrix attachment regions (MARs) can increase the expression of flanking transgenes. However, our previous studies revealed no effect of the chicken lysozyme MARs (chiMARs) on transgene expression in the first generation transgenic Arabidopsis thaliana plan...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: De Bolle MF,Butaye KM,Goderis IJ,Wouters PF,Jacobs A,Delauré SL,Depicker A,Cammue BP

    更新日期:2007-03-01 00:00:00

  • Axillary bud outgrowth in herbaceous shoots: how do strigolactones fit into the picture?

    abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Waldie T,Hayward A,Beveridge CA

    更新日期:2010-05-01 00:00:00

  • Organization, not duplication, triggers silencing in a complex transgene locus in rice.

    abstract::Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

    更新日期:2005-06-01 00:00:00

  • A role of OsGA20ox1 , encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice.

    abstract::Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Oikawa T,Koshioka M,Kojima K,Yoshida H,Kawata M

    更新日期:2004-07-01 00:00:00

  • The auxin-induced K(+) channel gene Zmk1 in maize functions in coleoptile growth and is required for embryo development.

    abstract::The transcript level and in turn protein density of the K(+)-uptake channel ZMK1 in maize (Zea mays) coleoptiles is controlled by the phytohormone auxin. ZMK1 is involved in auxin-regulated coleoptile elongation as well as gravi- and phototropism. To provide unequivocal evidence for the role of ZMK1 in these elementar...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Philippar K,Büchsenschütz K,Edwards D,Löffler J,Lüthen H,Kranz E,Edwards KJ,Hedrich R

    更新日期:2006-07-01 00:00:00

  • A transcript encoding translation initiation factor eIF-5A is stored in unfertilized egg cells of maize.

    abstract::Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Dresselhaus T,Cordts S,Lörz H

    更新日期:1999-03-01 00:00:00

  • The expression of an abundant transmitting tract-specific endoglucanase (Sp41) is promoter-dependent and not essential for the reproductive physiology of tobacco.

    abstract::In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sessa G,Fluhr R

    更新日期:1995-12-01 00:00:00

  • Anaerobiosis-specific interaction of tobacco nuclear factors with cis-regulatory sequences in the maize GapC4 promoter.

    abstract::The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Geffers R,Cerff R,Hehl R

    更新日期:2000-05-01 00:00:00

  • A novel zinc-finger protein with a proline-rich domain mediates ABA-regulated seed dormancy in Arabidopsis.

    abstract::Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: He Y,Gan S

    更新日期:2004-01-01 00:00:00

  • Isolation and characterization of a fruit-specific cDNA and the corresponding genomic clone from tomato.

    abstract::Differential screening of a cDNA bank constructed from ripe tomato fruit mRNA allowed the isolation of cDNA clone 2A11 which is entirely fruit-specific, is expressed at steadily increasing levels from anthesis to breaker, and accounts for approximately 1% of the messenger RNA in mature tomato fruit. A genomic clone co...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pear JR,Ridge N,Rasmussen R,Rose RE,Houck CM

    更新日期:1989-12-01 00:00:00

  • Tetracycline-dependent activation of an upstream promoter reveals transcriptional interference between tandem genes within T-DNA in tomato.

    abstract::Tomato plants stably expressing the tetR gene were transformed with the beta-glucuronidase (GUS) gene and an le20 antisense construct both under the control of the Triple-Op promoter. Induction of GUS mRNA and le20 antisense RNA in leaves was demonstrated in response to tetracycline. le20 antisense induction was also ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Thompson AJ,Myatt SC

    更新日期:1997-07-01 00:00:00

  • Phytochrome-mediated activation of the gene for cytosolic glutamine-synthetase (GS1) during imbibition of photosensitive lettuce seeds.

    abstract::A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glu...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sakamoto A,Takeba G,Shibata D,Tanaka K

    更新日期:1990-08-01 00:00:00

  • The β-conglycinin deficiency in wild soybean is associated with the tail-to-tail inverted repeat of the α-subunit genes.

    abstract::β-conglycinin, a major seed protein in soybean, is composed of α, α', and β subunits sharing a high homology among them. Despite its many health benefits, β-conglycinin has a lower amino acid score and lower functional gelling properties compared to glycinin, another major soybean seed protein. In addition, the α, α',...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Tsubokura Y,Hajika M,Kanamori H,Xia Z,Watanabe S,Kaga A,Katayose Y,Ishimoto M,Harada K

    更新日期:2012-02-01 00:00:00

  • Isolation and characterization of P-type H(+)-ATPase genes from potato.

    abstract::H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Harms K,Wöhner RV,Schulz B,Frommer WB

    更新日期:1994-11-01 00:00:00

  • Characterization of pectinases and pectin methylesterase cDNAs in pods of green beans (Phaseolus vulgaris L.).

    abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ebbelaar ME,Tucker GA,Laats MM,van Dijk C,Stolle-Smits T,Recourt K

    更新日期:1996-09-01 00:00:00

  • The regulatory mechanism of chilling-induced dormancy transition from endo-dormancy to non-dormancy in Polygonatum kingianum Hemsl rhizome bud.

    abstract:KEY MESSAGE:We identified three dormant stages of Polygonatum kingianum and changes that occurred during dormancy transition in the following aspects including cell wall and hormones, as well as interaction among them. Polygonatum kingianum Hemsl (P. kingianum) is an important traditional Chinese medicine, but ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Wang Y,Liu X,Su H,Yin S,Han C,Hao D,Dong X

    更新日期:2019-02-01 00:00:00

  • Molecular cloning and characterization of RAD51-like genes from Arabidopsis thaliana.

    abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Osakabe K,Yoshioka T,Ichikawa H,Toki S

    更新日期:2002-09-01 00:00:00

  • Phylogenetic origin of a secondary pathway: the case of pyrrolizidine alkaloids.

    abstract::Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Ober D,Hartmann T

    更新日期:2000-11-01 00:00:00

  • A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer.

    abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van der Geest AH,Hall TC

    更新日期:1996-11-01 00:00:00

  • Correction to: Heat stress alters genome-wide profiles of circular RNAs in Arabidopsis.

    abstract::Due to an unfortunate turn of events, the first name of the fifth author appeared incorrectly in the original publication and should have read Guangbing. The correct representation of the authors' names and their affiliation is listed here and should be treated as definitive. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,已发布勘误


    authors: Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

    更新日期:2018-02-01 00:00:00

  • Novel protein kinase of Arabidopsis thaliana (APK1) that phosphorylates tyrosine, serine and threonine.

    abstract::During the course of characterizing polymerase chain reaction products corresponding to protein kinases of a higher plant, Arabidopsis thaliana, we found a DNA fragment that potentially codes for a polypeptide with mosaic sequences of two classes of protein kinases, a tyrosine-specific and a serine/threonine-specific ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Hirayama T,Oka A

    更新日期:1992-11-01 00:00:00

  • Structure and expression of a gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) in the colourless euglenoid flagellate Astasia longa.

    abstract::A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Siemeister G,Hachtel W

    更新日期:1990-05-01 00:00:00

  • Molecular cloning and characterization of a legumin-like storage protein cDNA of Douglas fir seeds.

    abstract::A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Leal I,Misra S

    更新日期:1993-02-01 00:00:00

  • Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme.

    abstract::Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Henze K,Schnarrenberger C,Kellermann J,Martin W

    更新日期:1994-12-01 00:00:00

  • Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.

    abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: McNeilly D,Schofield A,Stone SL

    更新日期:2018-02-01 00:00:00

  • Transcriptome and weighted correlation network analyses provide insights into inflorescence stem straightness in Paeonia lactiflora.

    abstract:KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

    更新日期:2020-02-01 00:00:00

  • Context sequences of translation initiation codon in plants.

    abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Joshi CP,Zhou H,Huang X,Chiang VL

    更新日期:1997-12-01 00:00:00