Abstract:
:The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension analyses. The arrangement and expression of the PE alpha and beta genes, named rpeA and rpeB, are similar to those of the cyanobacterial (C)-PE genes: rpeB is located 5' of rpeA, with an intergenic region of 64 nucleotides. The two genes are transcribed on a 1.25 kb dicistronic transcript, and each coding region is preceded by a prokaryotic ribosome binding site consensus sequence. Transcription is initiated 95 nucleotides upstream of the initiating methionine codon of rpeB. The promoter region resembles that of prokaryotic genes, with an AT-rich -10 sequence. A direct pentanucleotide repeat (5'-TGTTA-3') was found in the -35 region. This pentanucleotide is present upstream of all PE operons that have been characterized thus far. An extensive inverted repeat is present 3' of rpeA; inverted repeats are found downstream of all PE operons sequenced to date, although the sequence is not conserved. The deduced amino acid sequences from these genes provide complete sequences for an R-PE. Of the amino acid residues 85% are identical to those of bangeophycean (B)-PE from the unicellular red alga Porphyridium cruentum. Conserved residues include cysteines at the bilin attachment sites of C- and B-PEs, aspartates at positions postulated to interact with bilin chromophores, and an apparent consensus sequence for N-methylation of an asparagine residue in C-PEs.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Roell MK,Morse DEdoi
10.1007/BF00039617subject
Has Abstractpub_date
1993-01-01 00:00:00pages
47-58issue
1eissn
0167-4412issn
1573-5028journal_volume
21pub_type
杂志文章abstract::WRKY transcription factors are involved in various biological processes, such as development, metabolism and responses to stress. However, their exact roles in abiotic stress tolerance are largely unknown. Here, we demonstrated a working model for the function of a WRKY gene (ThWRKY4) from Tamarix hispida in the stres...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0063-y
更新日期:2013-07-01 00:00:00
abstract::The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040137
更新日期:1986-03-01 00:00:00
abstract::Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9017-y
更新日期:2006-09-01 00:00:00
abstract::Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023238
更新日期:1994-05-01 00:00:00
abstract::Ribulose bisphosphate carboxylase-oxygenase (Rubisco) is a key enzyme in the photosynthetic fixation of CO2 by the chloroplast. The synthesis of the enzyme is an example of the cooperation between the chloroplast and the nucleocytoplasmic compartments, as it is assembled from subunits encoded in the two respective gen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021302
更新日期:1986-01-01 00:00:00
abstract::The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019103
更新日期:1996-11-01 00:00:00
abstract::The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015875
更新日期:1987-09-01 00:00:00
abstract::A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016504
更新日期:1990-05-01 00:00:00
abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019121
更新日期:1995-10-01 00:00:00
abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018558
更新日期:1990-02-01 00:00:00
abstract::A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005830313272
更新日期:1997-10-01 00:00:00
abstract::The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023992
更新日期:1991-03-01 00:00:00
abstract::Many eukaryotic genomes have experienced ancient whole-genome duplication (WGD) followed by massive gene loss. These eliminations were not random since some gene families were preferentially retained as duplicates. The gene balance hypothesis suggests that those genes with dosage reduction can imbalance their interact...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9639-y
更新日期:2010-07-01 00:00:00
abstract::We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006196530079
更新日期:1999-05-01 00:00:00
abstract::The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00019401
更新日期:1990-06-01 00:00:00
abstract:KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0677-6
更新日期:2017-12-01 00:00:00
abstract::Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9456-3
更新日期:2009-05-01 00:00:00
abstract::In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0397-8
更新日期:2016-01-01 00:00:00
abstract::Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1011848528377
更新日期:2001-11-01 00:00:00
abstract:KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01002-1
更新日期:2020-07-01 00:00:00
abstract::Alpha-dioxygenases (α-DOX) catalyzing the primary oxygenation of fatty acids to oxylipins were recently found in plants. Here, the biological roles of the pepper α-DOX (Ca-DOX) gene, which is strongly induced during non-host pathogen infection in chili pepper, were examined. Virus-induced gene silencing demonstrated t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0575-3
更新日期:2017-03-01 00:00:00
abstract::Plants perceiving drought activate multiple responses to improve survival, including large-scale alterations in gene expression. This article reports on the roles in the drought response of two Arabidopsis thaliana homeodomain-leucine zipper class I genes; ATHB7 and ATHB12, both strongly induced by water-deficit and a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9956-4
更新日期:2012-11-01 00:00:00
abstract:KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00926-7
更新日期:2020-01-01 00:00:00
abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0691-8
更新日期:2018-02-01 00:00:00
abstract::Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9071-5
更新日期:2007-01-01 00:00:00
abstract::A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017808
更新日期:1996-01-01 00:00:00
abstract::The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0248-z
更新日期:2014-12-01 00:00:00
abstract::We have compared physical and genetic maps of the region around the legJ gene in pea. In this vicinity there are four B-type legumin genes, arranged as two close pairs. The detection of a recombination event within this gene cluster allows the orientation of this group of genes within the surrounding linkage group to ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00038999
更新日期:1993-04-01 00:00:00
abstract::Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028854
更新日期:1994-11-01 00:00:00
abstract::DNA amplification fingerprinting (DAF) using a high primer-to-template ratio and single, very short arbitrary primers, was used to generate amplified fragment length polymorphic markers (AFLP) in soybean (Glycine max (L.) Merr.). The inheritance of AFLPs was studied using a cross between the ancestral Glycine soja PI4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039524
更新日期:1994-10-01 00:00:00