Isolation of a novel Arabidopsis ozone-induced cDNA by differential display.

Abstract:

:We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants was detected at low levels in cotyledons, leaves, and flower buds and at higher levels in roots and mature flowers. AtOZI1 mRNA accumulation was transiently induced in leaves 3- to 5-fold within the first 6 h of ozone treatment. AtOZI1 mRNA accumulation was also transiently induced 3- to 6-fold by phytopathogenic Pseudomonas strains. Sequence analysis of AtOZI1 revealed that it encodes a 8.6 kDa basic protein that contains a putative signal peptide and two potential phosphorylation sites. Our results suggest that AtOZI1 represents a novel stress-related protein that accumulates in response to the production of active oxygen species.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Sharma YK,Davis KR

doi

10.1007/BF00019121

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

91-8

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

29

pub_type

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