OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.


:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcription factor, OsIRO2, is responsible for regulation of the genes involved in Fe homeostasis in rice. Using promoter-GUS transformants, we showed that OsIRO2 is expressed throughout a plant's lifetime in a spatially and temporally similar manner to the genes OsNAS1, OsNAS2 and TOM1, which is involved in Fe absorption and translocation. During germination, OsIRO2 expression was detected in embryos. OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots and leaves, and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in response to Fe deficiency. OsIRO2 expression was also detected in flowers and developing seeds. Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination. OsIRO2 overexpression also resulted in improved tolerance to low Fe availability in calcareous soil. In addition to increased Fe content in shoots, the overexpression plants accumulated higher amounts of Fe in seeds than non-transformants when grown on calcareous soil. These results suggest that OsIRO2 is synchronously expressed with genes involved in Fe homeostasis, and performs a crucial function in regulation not only of Fe uptake from soil but also Fe transport during germination and Fe translocation to grain during seed maturation.


Plant Mol Biol


Plant molecular biology


Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK




Has Abstract


2011-04-01 00:00:00












  • Inducible expression of p50 from TMV for increased resistance to bacterial crown gall disease in tobacco.

    abstract::The dominant tobacco mosaic virus (TMV) resistance gene N induces a hypersensitive response upon TMV infection and protects tobacco against systemic spread of the virus. It has been proposed to change disease resistance specificity by reprogramming the expression of resistance genes or their corresponding avirulence g...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Niemeyer J,Ruhe J,Machens F,Stahl DJ,Hehl R

    更新日期:2014-01-01 00:00:00

  • Correction to: Heat stress alters genome-wide profiles of circular RNAs in Arabidopsis.

    abstract::Due to an unfortunate turn of events, the first name of the fifth author appeared incorrectly in the original publication and should have read Guangbing. The correct representation of the authors' names and their affiliation is listed here and should be treated as definitive. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,已发布勘误


    authors: Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

    更新日期:2018-02-01 00:00:00

  • Differential ethylene-inducible expression of cellulase in pepper plants.

    abstract::Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

    更新日期:1995-11-01 00:00:00

  • Flavonoid profiling among wild type and related GM wheat varieties.

    abstract::Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ioset JR,Urbaniak B,Ndjoko-Ioset K,Wirth J,Martin F,Gruissem W,Hostettmann K,Sautter C

    更新日期:2007-11-01 00:00:00

  • Isolation of cDNA clones of genes induced upon transfer of Chlamydomonas reinhardtii cells to low CO2.

    abstract::Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Burow MD,Chen ZY,Mouton TM,Moroney JV

    更新日期:1996-05-01 00:00:00

  • Expression of a rice glutelin promoter in transgenic tobacco.

    abstract::A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Leisy DJ,Hnilo J,Zhao Y,Okita TW

    更新日期:1990-01-01 00:00:00

  • A GARP transcription factor anther dehiscence defected 1 (OsADD1) regulates rice anther dehiscence.

    abstract::Anther dehiscence, one of the essential steps in pollination and double fertilization, is regulated by a complex signaling pathway encompassing hormones and environmental factors. However, key components underlying the signaling pathway that regulate anther dehiscence remain largely elusive. Here, we isolated a rice m...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Xiao Y,You S,Kong W,Tang Q,Bai W,Cai Y,Zheng H,Wang C,Jiang L,Wang C,Zhao Z,Wan J

    更新日期:2019-11-01 00:00:00

  • Agrobacterium-mediated sorghum transformation.

    abstract::Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

    更新日期:2000-12-01 00:00:00

  • The reconstruction and biochemical characterization of ancestral genes furnish insights into the evolution of terpene synthase function in the Poaceae.

    abstract:KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

    更新日期:2020-09-01 00:00:00

  • The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptides localized in Photosystem II.

    abstract::The photosynthetic apparatus of plant chloroplasts contains two photosystems, termed Photosystem I (PSI) and Photosystem II (PSII). Both PSI and PSII contain several types of chlorophyll a/b-binding (CAB) polypeptides, at least some of which are structurally related. It has been previously shown that multiple genes en...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pichersky E,Hoffman NE,Malik VS,Bernatzky R,Tanksley SD,Szabo L,Cashmore AR

    更新日期:1987-03-01 00:00:00

  • Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

    abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Atanassova R,Flénet M,Gigot C,Chaubet N

    更新日期:1998-05-01 00:00:00

  • CaaX-prenyltransferases are essential for expression of genes involvedin the early stages of monoterpenoid biosynthetic pathway in Catharanthus roseus cells.

    abstract::CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Courdavault V,Thiersault M,Courtois M,Gantet P,Oudin A,Doireau P,St-Pierre B,Giglioli-Guivarc'h N

    更新日期:2005-04-01 00:00:00

  • Genome-wide gene expression in an Arabidopsis cell suspension.

    abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Menges M,Hennig L,Gruissem W,Murray JA

    更新日期:2003-11-01 00:00:00

  • Isolation of a subfamily of genes for R2R3-MYB transcription factors showing up-regulated expression under nitrogen nutrient-limited conditions.

    abstract::Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

    更新日期:2003-09-01 00:00:00

  • Dihydrofolate reductase from Daucus carota cell suspension cultures: purification, molecular and kinetic characterization.

    abstract::The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Albani D,Parisi B,Carbonera D,Cella R

    更新日期:1985-11-01 00:00:00

  • The molecular and biochemical basis for varietal variation in sesquiterpene content in melon (Cucumis melo L.) rinds.

    abstract::A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Portnoy V,Benyamini Y,Bar E,Harel-Beja R,Gepstein S,Giovannoni JJ,Schaffer AA,Burger J,Tadmor Y,Lewinsohn E,Katzir N

    更新日期:2008-04-01 00:00:00

  • Intron-mediated enhancement of heterologous gene expression in maize.

    abstract::Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Mascarenhas D,Mettler IJ,Pierce DA,Lowe HW

    更新日期:1990-12-01 00:00:00

  • The Arabidopsis PPDK gene is transcribed from two promoters to produce differentially expressed transcripts responsible for cytosolic and plastidic proteins.

    abstract::Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Parsley K,Hibberd JM

    更新日期:2006-10-01 00:00:00

  • Structure of the parsley caffeoyl-CoA O-methyltransferase gene, harbouring a novel elicitor responsive cis-acting element.

    abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Grimmig B,Matern U

    更新日期:1997-01-01 00:00:00

  • Characterization of common cis-regulatory elements responsible for the endosperm-specific expression of members of the rice glutelin multigene family.

    abstract::Glutelin is the most abundant storage protein in rice, which is expressed specifically in the endosperm of maturing seed. Glutelin is encoded by about 10 genes per haploid genome, which are clearly divided into two subfamilies (GluA and GluB). Most of them are coordinately expressed during seed maturation in spite of ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Takaiwa F,Yamanouchi U,Yoshihara T,Washida H,Tanabe F,Kato A,Yamada K

    更新日期:1996-03-01 00:00:00

  • Independent encapsidation of tomato golden mosaic virus A component DNA in transgenic plants.

    abstract::Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

    更新日期:1987-11-01 00:00:00

  • A novel male-sterile mutant of Arabidopsis thaliana, faceless pollen-1, produces pollen with a smooth surface and an acetolysis-sensitive exine.

    abstract::A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Ariizumi T,Hatakeyama K,Hinata K,Sato S,Kato T,Tabata S,Toriyama K

    更新日期:2003-09-01 00:00:00

  • Cloning and characterization of a second form of the rice adenine phosphoribosyl transferase gene (OsAPT2) and its association with TGMS.

    abstract::A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zhou CJ,Li J,Zou JC,Liang FS,Ye CJ,Jin DM,Weng ML,Wang B

    更新日期:2006-02-01 00:00:00

  • Pea lectin receptor-like kinase functions in salinity adaptation without yield penalty, by alleviating osmotic and ionic stresses and upregulating stress-responsive genes.

    abstract::Lectin receptor-like kinases (LecRLKs) are members of RLK family composed of lectin-like extracellular recognition domain, transmembrane domain and cytoplasmic kinase domain. LecRLKs are plasma membrane proteins believed to be involved in signal transduction. However, most of the members of the protein family even in ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Vaid N,Pandey P,Srivastava VK,Tuteja N

    更新日期:2015-05-01 00:00:00

  • Molecular biology of fruit ripening and its manipulation with antisense genes.

    abstract::Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Gray J,Picton S,Shabbeer J,Schuch W,Grierson D

    更新日期:1992-05-01 00:00:00

  • Phenotypic and gene expression analyses of a ploidy series of maize inbred Oh43.

    abstract::Polyploidization has repeatedly occurred during plant evolution. Although autopolyploidy is the best model to characterize the polyploidization effects in a highly controlled manner, there are limited studies on autopolyploids compared to allopolyploids. To improve our understanding of autopolyploidy effects in maize,...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Yao H,Kato A,Mooney B,Birchler JA

    更新日期:2011-02-01 00:00:00

  • Nutritional functions of the funiculus in Brassica napus seed maturation revealed by transcriptome and dynamic metabolite profile analyses.

    abstract::The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Tan H,Xiang X,Tang J,Wang X

    更新日期:2016-11-01 00:00:00

  • Identification of Mutator insertional mutants of starch-branching enzyme 1 (sbe1) in Zea mays L.

    abstract::Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

    更新日期:2002-02-01 00:00:00

  • Molecular cloning and functional characterization of genes associated with flowering in citrus using an early-flowering trifoliate orange (Poncirus trifoliata L. Raf.) mutant.

    abstract::To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Zhang JZ,Ai XY,Sun LM,Zhang DL,Guo WW,Deng XX,Hu CG

    更新日期:2011-05-01 00:00:00

  • The elongation defective1 mutant of Arabidopsis is impaired in the gene encoding a serine-rich secreted protein.

    abstract::Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lertpiriyapong K,Sung ZR

    更新日期:2003-11-01 00:00:00