OsIRO2 is responsible for iron utilization in rice and improves growth and yield in calcareous soil.


:Iron (Fe) deficiency, a worldwide agricultural problem on calcareous soil with low Fe availability, is also a major human nutritional deficit. Plants induce Fe acquisition systems under conditions of low Fe availability. Previously, we reported that an Fe-deficiency-inducible basic helix-loop-helix (bHLH) transcription factor, OsIRO2, is responsible for regulation of the genes involved in Fe homeostasis in rice. Using promoter-GUS transformants, we showed that OsIRO2 is expressed throughout a plant's lifetime in a spatially and temporally similar manner to the genes OsNAS1, OsNAS2 and TOM1, which is involved in Fe absorption and translocation. During germination, OsIRO2 expression was detected in embryos. OsIRO2 expression in vegetative tissues was restricted almost exclusively to vascular bundles of roots and leaves, and to the root exodermis under Fe-sufficient conditions, and expanded to all tissues of roots and leaves in response to Fe deficiency. OsIRO2 expression was also detected in flowers and developing seeds. Plants overexpressing OsIRO2 grew better, and OsIRO2-repressed plants showed poor growth compared to non-transformant rice after germination. OsIRO2 overexpression also resulted in improved tolerance to low Fe availability in calcareous soil. In addition to increased Fe content in shoots, the overexpression plants accumulated higher amounts of Fe in seeds than non-transformants when grown on calcareous soil. These results suggest that OsIRO2 is synchronously expressed with genes involved in Fe homeostasis, and performs a crucial function in regulation not only of Fe uptake from soil but also Fe transport during germination and Fe translocation to grain during seed maturation.


Plant Mol Biol


Plant molecular biology


Ogo Y,Itai RN,Kobayashi T,Aung MS,Nakanishi H,Nishizawa NK




Has Abstract


2011-04-01 00:00:00












  • Glucosinolate hydrolysis in Lepidium sativum--identification of the thiocyanate-forming protein.

    abstract::Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Burow M,Bergner A,Gershenzon J,Wittstock U

    更新日期:2007-01-01 00:00:00

  • The protein phosphatase 2A catalytic subunit StPP2Ac2b acts as a positive regulator of tuberization induction in Solanum tuberosum L.

    abstract:KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Muñiz García MN,Muro MC,Mazzocchi LC,País SM,Stritzler M,Schlesinger M,Capiati DA

    更新日期:2017-02-01 00:00:00

  • Information contents and dinucleotide compositions of plant intron sequences vary with evolutionary origin.

    abstract::The DNA sequence composition of 526 dicot and 345 monocot intron sequences have been characterized using computational methods. Splice site information content and bulk intron and exon dinucleotide composition were determined. Positions 4 and 5 of 5' splice sites contain different statistically significant levels of i...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: White O,Soderlund C,Shanmugan P,Fields C

    更新日期:1992-09-01 00:00:00

  • Large-scale analysis of the GRAS gene family in Arabidopsis thaliana.

    abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Lee MH,Kim B,Song SK,Heo JO,Yu NI,Lee SA,Kim M,Kim DG,Sohn SO,Lim CE,Chang KS,Lee MM,Lim J

    更新日期:2008-08-01 00:00:00

  • Isolation of a novel Arabidopsis ozone-induced cDNA by differential display.

    abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Sharma YK,Davis KR

    更新日期:1995-10-01 00:00:00

  • Correction to: Heat stress alters genome-wide profiles of circular RNAs in Arabidopsis.

    abstract::Due to an unfortunate turn of events, the first name of the fifth author appeared incorrectly in the original publication and should have read Guangbing. The correct representation of the authors' names and their affiliation is listed here and should be treated as definitive. ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,已发布勘误


    authors: Pan T,Sun X,Liu Y,Li H,Deng G,Lin H,Wang S

    更新日期:2018-02-01 00:00:00

  • Simple binary vectors for DNA transfer to plant cells.

    abstract::Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van den Elzen P,Lee KY,Townsend J,Bedbrook J

    更新日期:1985-05-01 00:00:00

  • A putative beta-glucanase pseudogene behind the potato GBSS gene.

    abstract::We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: van der Leij FR,Abeln EC,Hesseling-Meinders A,Feenstra WJ

    更新日期:1993-02-01 00:00:00

  • Organization, expression and nucleotide sequence of the operon encoding R-phycoerythrin alpha and beta subunits from the red alga Polysiphonia boldii.

    abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Roell MK,Morse DE

    更新日期:1993-01-01 00:00:00

  • A model for the evolution of the plastid sec apparatus inferred from secY gene phylogeny.

    abstract::Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Vogel H,Fischer S,Valentin K

    更新日期:1996-11-01 00:00:00

  • The tomato brassinosteroid receptor BRI1 increases binding of systemin to tobacco plasma membranes, but is not involved in systemin signaling.

    abstract::The tomato wound signal systemin is perceived by a specific high-affinity, saturable, and reversible cell surface receptor. This receptor was identified as the receptor-like kinase SR160, which turned out to be identical to the brassinosteroid receptor BRI1. Recently, it has been shown that the tomato bri1 null mutant...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Malinowski R,Higgins R,Luo Y,Piper L,Nazir A,Bajwa VS,Clouse SD,Thompson PR,Stratmann JW

    更新日期:2009-07-01 00:00:00

  • Circadian and light-regulated expression of nitrate reductase in Arabidopsis.

    abstract::The expression of a number of plant genes is regulated by an endogenous circadian clock. We report that the Arabidopsis NIA2 (nitrate reductase) gene shows robust circadian oscillations in mRNA accumulation which persist for at least 5 days in plants that have been grown in a light-dark (LD) cycle and then transformed...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pilgrim ML,Caspar T,Quail PH,McClung CR

    更新日期:1993-10-01 00:00:00

  • EGY1 plays a role in regulation of endodermal plastid size and number that are involved in ethylene-dependent gravitropism of light-grown Arabidopsis hypocotyls.

    abstract::Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Guo D,Gao X,Li H,Zhang T,Chen G,Huang P,An L,Li N

    更新日期:2008-03-01 00:00:00

  • Cytoplasmic male sterility in sunflower: comparison of molecular biology and genetic studies.

    abstract::The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Crouzillat D,de la Canal L,Perrault A,Ledoigt G,Vear F,Serieys H

    更新日期:1991-03-01 00:00:00

  • Characterization of a pine multigene family containing elicitor-responsive stilbene synthase genes.

    abstract::Young pine seedlings respond to environmental stress by induced synthesis of pinosylvin, a stilbene phytoalexin. Heartwood of pine trees is characterized by a high content of pinosylvin. The formation of pinosylvin from cinnamoyl-CoA and three molecules malonyl-CoA catalysed by pinosylvin synthase is typical of the ge...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Preisig-Müller R,Schwekendiek A,Brehm I,Reif HJ,Kindl H

    更新日期:1999-01-01 00:00:00

  • Expression of stress-responsive ubiquitin genes in potato tubers.

    abstract::The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension prot...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Garbarino JE,Rockhold DR,Belknap WR

    更新日期:1992-10-01 00:00:00

  • Restriction fragment polymorphisms as probes for plant diversity and their development as tools for applied plant breeding.

    abstract::Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Helentjaris T,King G,Slocum M,Siedenstrang C,Wegman S

    更新日期:1985-03-01 00:00:00

  • Cytokinin content and tissue distribution in plants transformed by a reconstructed isopentenyl transferase gene.

    abstract::The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Smigocki AC

    更新日期:1991-01-01 00:00:00

  • Osmium tetroxide footprinting of a scaffold attachment region in the maize Adh1 promoter.

    abstract::Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Paul AL,Ferl RJ

    更新日期:1993-09-01 00:00:00

  • Common occurrence of homologues of petunia glycine-rich protein-1 among plants.

    abstract::The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Cheng SH,Keller B,Condit CM

    更新日期:1996-04-01 00:00:00

  • Sequential expression of two 1-aminocyclopropane-1-carboxylate synthase genes in response to biotic and abiotic stresses in potato (Solanum tuberosum L.) leaves.

    abstract::Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Schlagnhaufer CD,Arteca RN,Pell EJ

    更新日期:1997-12-01 00:00:00

  • Expression of a rice glutelin promoter in transgenic tobacco.

    abstract::A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Leisy DJ,Hnilo J,Zhao Y,Okita TW

    更新日期:1990-01-01 00:00:00

  • Arabidopsis E2F1 binds a sequence present in the promoter of S-phase-regulated gene AtCDC6 and is a member of a multigene family with differential activities.

    abstract::Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: de Jager SM,Menges M,Bauer UM,Murra JA

    更新日期:2001-11-01 00:00:00

  • Regulation of cell death in flower petals.

    abstract::The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审


    authors: Rubinstein B

    更新日期:2000-10-01 00:00:00

  • The trihelix transcription factor OsGTγ-2 is involved adaption to salt stress in rice.

    abstract:KEY MESSAGE:OsGTγ-2, a trihelix transcription factor, is a positive regulator of rice responses to salt stress by regulating the expression of ion transporters. Salinity stress seriously restricts rice growth and yield. Trihelix transcription factors (GT factors) specifically bind to GT elements and play a diverse role...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Liu X,Wu D,Shan T,Xu S,Qin R,Li H,Negm M,Wu D,Li J

    更新日期:2020-07-01 00:00:00

  • Transgene silencing of invertedly repeated transgenes is released upon deletion of one of the transgenes involved.

    abstract::To analyse experimentally the correlation between transgene silencing and the presence of an inverted repeat in transgenic Arabidopsis thaliana plants, expression of the beta-glucuronidase (gus) gene was studied when present as a convergently transcribed inverted repeat or as a single copy in otherwise isogenic lines....

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: De Buck S,Van Montagu M,Depicker A

    更新日期:2001-07-01 00:00:00

  • AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis.

    abstract::Neutral/Alkaline invertases are unique to plant and photosynthetic bacteria. The function of Neutral/Alkaline invertases in plant development is not clear so far. In this study, we isolated an Arabidopsis (Col-0) mutant insensitive to osmotic stress-induced inhibition on lateral root growth. Map-based cloning reveals ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Qi X,Wu Z,Li J,Mo X,Wu S,Chu J,Wu P

    更新日期:2007-07-01 00:00:00

  • Expression analysis of a sucrose carrier in the germinating seedling of Ricinus communis.

    abstract::This study describes the expression of a sucrose carrier at various developmental stages in Ricinus communis. A partial-length cDNA clone, RcSUT1, was isolated by RT-PCR from Ricinus seedling RNA. This is almost identical to a sucrose carrier cDNA, Rscr1, which has previously been isolated by library screening. Howeve...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Bick JA,Neelam A,Smith E,Nelson SJ,Hall JL,Williams LE

    更新日期:1998-10-01 00:00:00

  • Rice-specific Argonaute 17 controls reproductive growth and yield-associated phenotypes.

    abstract:KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: Pachamuthu K,Swetha C,Basu D,Das S,Singh I,Sundar VH,Sujith TN,Shivaprasad PV

    更新日期:2021-01-01 00:00:00

  • Degradation of the stress-responsive enzyme formate dehydrogenase by the RING-type E3 ligase Keep on Going and the ubiquitin 26S proteasome system.

    abstract:KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章


    authors: McNeilly D,Schofield A,Stone SL

    更新日期:2018-02-01 00:00:00