Disruption of a Upf1-like helicase-encoding gene OsPLS2 triggers light-dependent premature leaf senescence in rice.

abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

journal_title：Plant molecular biology

authors： Hsieh MH,Chang CY,Hsu SJ,Chen JJ

更新日期：2008-04-01 00:00:00

abstract：:As a highly active plant viral promoter that is able to function in a wide variety of cell types, the cauliflower mosaic virus (CaMV) 35S promoter has the potential for harboring a plant enhancer element. We tested this possibility and demonstrated that a 338 base pair fragment isolated from the region upstream of the...

journal_title：Plant molecular biology

authors： Odell JT,Knowlton S,Lin W,Mauvais CJ

更新日期：1988-05-01 00:00:00

abstract：:The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...

journal_title：Plant molecular biology

authors： Ohtsubo N,Nakayama T,Terada R,Shimamoto K,Iwabuchi M

更新日期：1993-11-01 00:00:00

abstract：:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...

journal_title：Plant molecular biology

authors： Sheen JY,Sayre RT,Bogorad L

更新日期：1987-05-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：:We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...

journal_title：Plant molecular biology

authors： Edwards KJ,Thompson H,Edwards D,de Saizieu A,Sparks C,Thompson JA,Greenland AJ,Eyers M,Schuch W

更新日期：1992-05-01 00:00:00

abstract：:Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...

journal_title：Plant molecular biology

authors： Meng Q,Du J,Li J,Lü X,Zeng X,Yuan M,Mao T

更新日期：2010-12-01 00:00:00

abstract：:Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

journal_title：Plant molecular biology

authors： Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

更新日期：2005-05-01 00:00:00

abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...

journal_title：Plant molecular biology

authors： Foley RC,Liang ZM,Singh KB

更新日期：1997-03-01 00:00:00

abstract：:The purification of dihydrofolate reductase (5, 6, 7, 8 tetrahydrofolate: NADP(+) oxidoreductase, E.C.: 1.5.1.3) from Daucus carota to apparent homogeneity, is described. The enzyme is a soluble protein with a molecular weight of 183 000±2 500, composed of identical subunits of 58 400±1 000. The enzyme is only weakly ...

journal_title：Plant molecular biology

authors： Albani D,Parisi B,Carbonera D,Cella R

更新日期：1985-11-01 00:00:00

abstract：:The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

journal_title：Plant molecular biology

authors： Görz A,Schäfer W,Hirasawa E,Kahl G

更新日期：1988-09-01 00:00:00

abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...

journal_title：Plant molecular biology

authors： Ward GC,Levings CS 3rd

更新日期：1991-11-01 00:00:00

abstract：:Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...

journal_title：Plant molecular biology

authors： Studart-Guimarães C,Gibon Y,Frankel N,Wood CC,Zanor MI,Fernie AR,Carrari F

更新日期：2005-11-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...

journal_title：Plant molecular biology

authors： Fraley RT,Horsch RB,Matzke A,Chilton MD,Chilton WS,Sanders PR

更新日期：1984-11-01 00:00:00

abstract：:In angiosperms the interactions between the secretory matrix of the stylar transmitting tract and the growing pollen tubes have central roles in determining a successful fertilization. Sp41 is a major glycosylated component of the soluble proteins of the transmitting tract matrix and exhibits (1-3)-beta-glucanase acti...

journal_title：Plant molecular biology

authors： Sessa G,Fluhr R

更新日期：1995-12-01 00:00:00

abstract：:Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...

journal_title：Plant molecular biology

authors： Johanningmeier U,Heiss S

更新日期：1993-04-01 00:00:00

abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...

journal_title：Plant molecular biology

authors： de Silva J,Robinson SJ,Safford R

更新日期：1992-04-01 00:00:00

abstract：:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

journal_title：Plant molecular biology

authors： Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

更新日期：2007-02-01 00:00:00

abstract：:Ethylene promotes the abscission of leaves and the ripening of fruits in pepper plants, and in both events an increase in cellulase activity is observed. However, two enzyme isoforms (pI 7.2 and 8.5, respectively) are differentially involved in the two physiological phenomena. The pI 8.5 form has been purified from ri...

journal_title：Plant molecular biology

authors： Ferrarese L,Trainotti L,Moretto P,Polverino de Laureto P,Rascio N,Casadoro G

更新日期：1995-11-01 00:00:00

abstract：:The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...

journal_title：Plant molecular biology

authors： Cutanda-Perez MC,Ageorges A,Gomez C,Vialet S,Terrier N,Romieu C,Torregrosa L

更新日期：2009-04-01 00:00:00

abstract：:The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...

journal_title：Plant molecular biology

authors： Kim J,Klein PG,Mullet JE

更新日期：1994-06-01 00:00:00

abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...

journal_title：Plant molecular biology

authors： Kirchherr D,Albrecht H,Mesnard JM,Lebeurier G

更新日期：1988-05-01 00:00:00

abstract：:When dark-grown aurea mutant tomato seedlings which lack more than 95% of the phytochrome present in isogenic wild-type seedlings are kept in white or blue light, four nuclear-encoded transcripts coding for plastidic proteins (the light-harvesting chlorophyll a/b-binding protein of photosystem I and II [cab-PSII], pla...

journal_title：Plant molecular biology

authors： Oelmüller R,Kendrick RE

更新日期：1991-02-01 00:00:00

abstract：:High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...

journal_title：Plant molecular biology

authors： Wakarchuk DA,Hamilton RI

更新日期：1985-01-01 00:00:00

abstract：:The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...

journal_title：Plant molecular biology

authors： Klämbt D

更新日期：1990-06-01 00:00:00

abstract：:A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...

journal_title：Plant molecular biology

authors： Zhou CJ,Li J,Zou JC,Liang FS,Ye CJ,Jin DM,Weng ML,Wang B

更新日期：2006-02-01 00:00:00

abstract：:Several populations of polyadenylated RNA from Vicia faba méristematic root cells were fractionated by stepwise thermal elution from poly(U)-Sepharose following sequential phenol extraction. Analysis of these fractions showed that the size of the poly(A) segment could influence this fractionation, but in some cases ot...

journal_title：Plant molecular biology

authors： Trapy G,Favre A,Esnault R

更新日期：1981-03-01 00:00:00

abstract：:We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a,...

journal_title：Plant molecular biology

authors： Saisho D,Nambara E,Naito S,Tsutsumi N,Hirai A,Nakazono M

更新日期：1997-11-01 00:00:00

abstract：:Drought is one of the critical factors limiting reproductive yields of rice and other crops globally. However, little is known about the molecular mechanism underlying reproductive development under drought stress in rice. To explore the potential gene function for improving rice reproductive development under drought...

journal_title：Plant molecular biology

authors： Guo C,Ge X,Ma H

更新日期：2013-06-01 00:00:00