Cellular double-stranded RNA in Phaseolus vulgaris.

abstract：:In this study, attempt has been made to produce a selected cultivar of tea with low-caffeine content using RNAi technology. The caffeine biosynthetic pathway in tea has been proposed to involve three N-methyltransferases such as xanthosine methyltransferase, 7-N-methylxanthine methyltransferase and 3, 7-dimethylxanthi...

journal_title：Plant molecular biology

authors： Mohanpuria P,Kumar V,Ahuja PS,Yadav SK

更新日期：2011-08-01 00:00:00

abstract：KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...

journal_title：Plant molecular biology

authors： Jian X,Zhao Y,Wang Z,Li S,Li L,Luo J,Kong L

更新日期：2020-10-01 00:00:00

abstract：:Sequences encoding the immunoglobulin heavy-chain variable (VH) domains were engineered in a new general purpose vector to transform plants via Agrobacterium. The expression of an isolated VH domain (IVD) after introduction into the plant genome has been monitored by northern, western and immunohistochemical analysis....

journal_title：Plant molecular biology

authors： Benvenuto E,Ordàs RJ,Tavazza R,Ancora G,Biocca S,Cattaneo A,Galeffi P

更新日期：1991-10-01 00:00:00

abstract：:Unilateral incompatibility often occurs between self-incompatible (SI) species and their self-compatible (SC) relatives. For example, SI Nicotiana alata rejects pollen from SC N. plumbaginifolia, but the reciprocal pollination is compatible. This interspecific pollen rejection system closely resembles intraspecific S-...

journal_title：Plant molecular biology

authors： Beecher B,Murfett J,McClure BA

更新日期：1998-03-01 00:00:00

abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...

journal_title：Plant molecular biology

authors： Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

更新日期：1995-04-01 00:00:00

abstract：:The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...

journal_title：Plant molecular biology

authors： Wang ZX,Yamanouchi U,Katayose Y,Sasaki T,Yano M

更新日期：2001-11-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

journal_title：Plant molecular biology

authors： Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

更新日期：2005-05-01 00:00:00

abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

journal_title：Plant molecular biology

authors： de Jager SM,Menges M,Bauer UM,Murra JA

更新日期：2001-11-01 00:00:00

abstract：:An Arabidopsis thaliana gene (UBC6) encoding a homologue to ubiquitin-conjugating enzymes has been isolated which is capable of encoding a protein of 183 amino acids of ca. 21 kDa. Northern analysis indicates that the gene is expressed in flowers, seeds and, to a somewhat lesser extent, in 10-day seedlings but not in ...

journal_title：Plant molecular biology

authors： Watts FZ,Butt N,Layfield P,Machuka J,Burke JF,Moore AL

更新日期：1994-10-01 00:00:00

abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...

journal_title：Plant molecular biology

authors： Leone M,Zavallo D,Venturuzzi A,Asurmendi S

更新日期：2020-11-01 00:00:00

abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...

journal_title：Plant molecular biology

authors： Chye ML

更新日期：1998-11-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification ...

journal_title：Plant molecular biology

authors： Or E,Vilozny I,Eyal Y,Ogrodovitch A

更新日期：2000-07-01 00:00:00

abstract：:The R and B genes of maize regulate the anthocyanin biosynthetic pathway and constitute a small gene family whose evolution has been shaped by polyploidization and transposable element activity. To compare the evolution of regulatory genes in the distinct but related genomes of rice and maize, we previously isolated t...

journal_title：Plant molecular biology

authors： Hu J,Reddy VS,Wessler SR

更新日期：2000-03-01 00:00:00

abstract：:In order to optimise expression of a foreign protein in transgenic plants we investigated the potential benefits of including a viral untranslated leader sequence within a plant transformation vector. A variety of 5 leaders, including the tobacco mosaic virus (TMV) leader sequence and 31 nucleotides of the cauliflower...

journal_title：Plant molecular biology

authors： Dowson Day MJ,Ashurst JL,Mathias SF,Watts JW,Wilson TM,Dixon RA

更新日期：1993-10-01 00:00:00

abstract：:Transgenic plants carrying chimeric genes composed of segments of the 5'-flanking region of the Arabidopsis 9-tubulin gene (TUB9) fused to the coding region of the beta-glucuronidase (GUS) gene of Escherichia coli were used to investigate the temporal and spatial patterns of TUB9 expression. Chimeric genes that contai...

journal_title：Plant molecular biology

authors： Cheng Z,Snustad DP,Carter JV

更新日期：2001-10-01 00:00:00

abstract：:Gametophytic apomictic plants form non-reduced embryo sacs that generate clonal embryos by parthenogenesis, in the absence of both meiosis and egg-cell fertilization. Here we report the sequence and expression analysis of a lorelei-like Paspalum notatum gene, n20gap-1, which encodes a GPI-anchored protein previously a...

journal_title：Plant molecular biology

authors： Felitti SA,Seijo JG,González AM,Podio M,Laspina NV,Siena L,Ortiz JP,Pessino SC

更新日期：2011-11-01 00:00:00

abstract：:We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...

journal_title：Plant molecular biology

authors： Karrer EE,Beachy RN,Holt CA

更新日期：1998-03-01 00:00:00

abstract：:We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...

journal_title：Plant molecular biology

authors： Fahrendorf T,Ni W,Shorrosh BS,Dixon RA

更新日期：1995-08-01 00:00:00

abstract：:A cDNA clone (lp3) from loblolly pine induced by water deficit stress (WDS) has been isolated. It is preferentially induced in roots with a constitutive basal level of expression also observed in stems and needles. Northern blot analysis with well irrigated ABA-treated seedlings indicated that the overall accumulation...

journal_title：Plant molecular biology

authors： Padmanabhan V,Dias DM,Newton RJ

更新日期：1997-12-01 00:00:00

abstract：:The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...

journal_title：Plant molecular biology

authors： Kuroda H,Sugiura M

更新日期：2014-12-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：KEY MESSAGE:The simultaneous expression of AmRosea1 and AmDelila transcription factors from snapdragon can activate the anthocyanin pathway in orange carrots, leading to the synthesis and accumulation of anthocyanins in the taproots. Anthocyanins are phenolic compounds produced in various parts of plants. They are used...

journal_title：Plant molecular biology

authors： Sharma S,Holme IB,Dionisio G,Kodama M,Dzhanfezova T,Joernsgaard B,Brinch-Pedersen H

更新日期：2020-07-01 00:00:00

abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

journal_title：Plant molecular biology

authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

更新日期：2001-08-01 00:00:00

abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...

journal_title：Plant molecular biology

authors： Christensen AB,Gregersen PL,Schröder J,Collinge DB

更新日期：1998-07-01 00:00:00

abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

journal_title：Plant molecular biology

authors： Sohal AK,Pallas JA,Jenkins GI

更新日期：1999-09-01 00:00:00

abstract：:The gene for the membrane polypeptide D2 has been mapped on the pea (Pisum sativum) chloroplast genome. The nucleotide sequence of the gene and its flanking regions is presented. The only large open reading frame in the sequence codes for a protein of MW 39.5 kD. A potential ribosome binding site is located 6 nucleoti...

journal_title：Plant molecular biology

authors： Rasmussen OF,Bookjans G,Stummann BM,Henningsen KW

更新日期：1984-07-01 00:00:00

abstract：:We have previously shown that two CRE elements situated on a 31 bp region of the cauliflower mosaic virus (CaMV) 35S promoter activate gene expression in the yeast Saccharomyces cerevisiae and are regulated by cAMP. Studies with the yeast transcription factors GCN4, SKO1 and YAP1, which bind CRE-like sequences, showed...

journal_title：Plant molecular biology

authors： Rüth J,Schweyen RJ,Hirt H

更新日期：1994-05-01 00:00:00

abstract：:Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...

journal_title：Plant molecular biology

authors： Schlagnhaufer CD,Arteca RN,Pell EJ

更新日期：1997-12-01 00:00:00