Expression of the CMS-associated urfS sequence in transgenic petunia and tobacco.

Abstract:

:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA which encodes the 25 kDa protein. The urfS sequence was fused with two different mitochondrial targeting sequences. The chimeric gene coding region was placed under the control of the CaMV 35S promoter or a tapetum-specific promoter. Expression of the PCF protein was obtained in mitochondria of transgenic petunia and tobacco plants, yet fertility of the plants was not affected. Analysis of the location of the urfS-encoded protein revealed that it fractionates primarily into the soluble fraction in the transgenic plants whereas the genuine 25 kDa protein is found primarily in the soluble fraction but also in the membrane portion of immature buds from CMS petunia plants. Fertile transgenic plants were obtained which expressed the 25 kDa protein in the tapetal layer of post-meiotic anthers, while CMS plants express the endogenous 25 kDa protein in both the tapetal layer and sporogenous tissue of pre-meiotic anthers.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Wintz H,Chen HC,Sutton CA,Conley CA,Cobb A,Ruth D,Hanson MR

doi

10.1007/BF00042040

subject

Has Abstract

pub_date

1995-04-01 00:00:00

pages

83-92

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

28

pub_type

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