Producing low-caffeine tea through post-transcriptional silencing of caffeine synthase mRNA.

abstract：:RNA silencing is a sequence-specific RNA degradation mechanism found in most eukaryotes, where small cleavage products (siRNAs) of double stranded RNA (dsRNA) mediate silencing of genes with sequence identity to the dsRNA inducer. In several systems, silencing has been found to spread from the dsRNA inducer sequence i...

journal_title：Plant molecular biology

authors： Petersen BO,Albrechtsen M

更新日期：2005-07-01 00:00:00

abstract：:We have characterized 4 of the 16 members of the family of dynamin-related proteins (DRP) in Arabidopsis. Three members, DRP1A (previously referred as ADL1), DRP1C and DRP1E, belong to the largest group of phragmoplastin-like proteins. DRP2A (ADL6) is one of the two members that contain a pleckstrin homology (PH) doma...

journal_title：Plant molecular biology

authors： Hong Z,Geisler-Lee CJ,Zhang Z,Verma DP

更新日期：2003-10-01 00:00:00

abstract：:The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...

journal_title：Plant molecular biology

authors： Tewari RK,Satoh M,Kado S,Mishina K,Anma M,Enami K,Hanaoka M,Watanabe M

更新日期：2014-12-01 00:00:00

abstract：:Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for alpha...

journal_title：Plant molecular biology

authors： Studart-Guimarães C,Gibon Y,Frankel N,Wood CC,Zanor MI,Fernie AR,Carrari F

更新日期：2005-11-01 00:00:00

abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...

journal_title：Plant molecular biology

authors： Bastianelli F,Costa A,Vescovi M,D'Apuzzo E,Zottini M,Chiurazzi M,Lo Schiavo F

更新日期：2010-03-01 00:00:00

abstract：:The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...

journal_title：Plant molecular biology

authors： Vincentz M,Leite A,Neshich G,Vriend G,Mattar C,Barros L,Weinberg D,de Almeida ER,de Carvalho MP,Aragão F,Gander ES

更新日期：1997-08-01 00:00:00

abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...

journal_title：Plant molecular biology

authors： Bustos MM,Luckow VA,Griffing LR,Summers MD,Hall TC

更新日期：1988-11-01 00:00:00

abstract：:Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

journal_title：Plant molecular biology

authors： Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

更新日期：1995-09-01 00:00:00

abstract：:We have previously shown that the mitochondrial gene atpA, encoding the alpha subunit of F1 ATP synthase, is associated with DNA rearrangements and nuclear-specific transcript patterns in the male-sterile cytoplasm of Ogura radish. Here we present a detailed characterization of this gene from both the normal (fertile)...

journal_title：Plant molecular biology

authors： Makaroff CA,Apel IJ,Palmer JD

更新日期：1990-11-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：:The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...

journal_title：Plant molecular biology

authors： Woessner JP,Gillham NW,Boynton JE

更新日期：1987-03-01 00:00:00

abstract：:The Guillardia theta chloroplast hlpA gene encodes a protein resembling bacterial histone-like protein HU. This gene was cloned and overexpressed in Escherichia coli cells, and the resulting protein product, HlpA, was purified and characterized in vitro. In addition to exhibiting a general DNA-binding activity, the ch...

journal_title：Plant molecular biology

authors： Wu H,Liu XQ

更新日期：1997-05-01 00:00:00

abstract：:Endogenous, 14 kb double-stranded RNAs (dsRNAs) have been found in two ecospecies of cultivated rice (temperate japonica rice and tropical japonica rice, Oryza sativa L.) and in wild rice (O. rufipogon, an ancestor of O. sativa). A comparison of the nucleotide and deduced amino acid sequences of the core regions of th...

journal_title：Plant molecular biology

authors： Moriyama H,Horiuchi H,Nitta T,Fukuhara T

更新日期：1999-04-01 00:00:00

abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

journal_title：Plant molecular biology

authors： Morel JB,Vaucheret H

更新日期：2000-06-01 00:00:00

abstract：:A MADS-box gene was isolated using the suppressive subtractive hybridization library between early-flowering mutant and wild-type trifoliate orange (Poncirus trifoliata L. Raf.). This gene is highly homologous with Arabidopsis SHORT VEGETATIVE PHASE (SVP). Based on real-time PCR and in situ hybridization during bud di...

journal_title：Plant molecular biology

authors： Li ZM,Zhang JZ,Mei L,Deng XX,Hu CG,Yao JL

更新日期：2010-09-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...

journal_title：Plant molecular biology

authors： Costa CL,Arruda P,Benedetti CE

更新日期：2000-09-01 00:00:00

abstract：:The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...

journal_title：Plant molecular biology

authors： Cheng SH,Keller B,Condit CM

更新日期：1996-04-01 00:00:00

abstract：:A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...

journal_title：Plant molecular biology

authors： Barre A,Van Damme EJ,Peumans WJ,Rougé P

更新日期：1997-03-01 00:00:00

abstract：KEY MESSAGE:TaPSK5 is a less conserved target of miR164 in wheat encoding a positive regulator of root growth and yield traits that could be used for crop improvement. MicroRNAs (miRNAs) play key roles in regulating plant growth and development by targeting the mRNAs of conserved genes. However, little is known about t...

journal_title：Plant molecular biology

authors： Geng Y,Jian C,Xu W,Liu H,Hao C,Hou J,Liu H,Zhang X,Li T

更新日期：2020-12-01 00:00:00

abstract：:In the present study, we utilized TALEN- and CRISPR/Cas9-induced mutations to analyze the promoter of the barley phytase gene HvPAPhy_a. The purpose of the study was dual, validation of the PAPhy_a enzyme as the main contributor of the mature grain phytase activity (MGPA), as well as validating the importance of a spe...

journal_title：Plant molecular biology

authors： Holme IB,Wendt T,Gil-Humanes J,Deleuran LC,Starker CG,Voytas DF,Brinch-Pedersen H

更新日期：2017-09-01 00:00:00

abstract：:Free Air [CO(2)] Enrichment (FACE) allows for plant growth under fully open-air conditions of elevated [CO(2)] at concentrations expected to be reached by mid-century. We used Arabidopsis thaliana ecotypes Col-0, Cvi-0, and WS to analyze changes in gene expression and metabolite profiles of plants grown in "SoyFACE" (...

journal_title：Plant molecular biology

authors： Li P,Sioson A,Mane SP,Ulanov A,Grothaus G,Heath LS,Murali TM,Bohnert HJ,Grene R

更新日期：2006-11-01 00:00:00

abstract：:The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...

journal_title：Plant molecular biology

authors： Görz A,Schäfer W,Hirasawa E,Kahl G

更新日期：1988-09-01 00:00:00

abstract：:A chimeric gene consisting of a bean (Phaseolus vulgaris L.) chalcone synthase (CHS) promoter fused to a bacterial chloramphenicol acetyltransferase (CAT) reporter gene was strongly expressed, and further induced by fungal elicitor, when electroporated into alfalfa (Medicago sativa L.) suspension cell protoplasts. Fun...

journal_title：Plant molecular biology

authors： Harrison MJ,Choudhary AD,Dubery I,Lamb CJ,Dixon RA

更新日期：1991-05-01 00:00:00

abstract：:Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...

journal_title：Plant molecular biology

authors： Xie Q,Sanz-Burgos AP,Guo H,García JA,Gutiérrez C

更新日期：1999-03-01 00:00:00

abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...

journal_title：Plant molecular biology

authors： Gil P,Green PJ

更新日期：1997-07-01 00:00:00

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...

journal_title：Plant molecular biology

authors： Naing AH,Kim CK

更新日期：2018-09-01 00:00:00

abstract：:Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...

journal_title：Plant molecular biology

authors： Yuan F,Lyu MJ,Leng BY,Zhu XG,Wang BS

更新日期：2016-06-01 00:00:00

abstract：:When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...

journal_title：Plant molecular biology

authors： Ishitani M,Nakamura T,Han SY,Takabe T

更新日期：1995-01-01 00:00:00