Post-transcriptional gene silencing mutants.

abstract：:In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line o...

journal_title：Plant molecular biology

authors： Gaude T,Rougier M,Heizmann P,Ockendon DJ,Dumas C

更新日期：1995-03-01 00:00:00

abstract：:The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

journal_title：Plant molecular biology

authors： Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

更新日期：1987-01-01 00:00:00

abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...

journal_title：Plant molecular biology

authors： Kohno-Murase J,Murase M,Ichikawa H,Imamura J

更新日期：1994-11-01 00:00:00

abstract：:We have characterized a gene specifically expressed in the floral nectaries of Brassica campestris L. ssp. pekinensis. Differential screening led to the isolation of a floral nectary-specific cDNA clone. Northern hybridization indicated that its mRNA transcript is 1450 nucleotides long and specific to the flower base....

journal_title：Plant molecular biology

authors： Song JT,Seo HS,Song SI,Lee JS,Choi YD

更新日期：2000-03-01 00:00:00

abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...

journal_title：Plant molecular biology

authors： Blanco F,Salinas P,Cecchini NM,Jordana X,Van Hummelen P,Alvarez ME,Holuigue L

更新日期：2009-05-01 00:00:00

abstract：:Cistus creticus subsp. creticus is a plant of intrinsic scientific interest due to the distinctive pharmaceutical properties of its resin. Labdane-type diterpenes, the main constituents of the resin, exhibit considerable antibacterial and cytotoxic activities. In this study chemical analysis of isolated trichomes from...

journal_title：Plant molecular biology

authors： Falara V,Fotopoulos V,Margaritis T,Anastasaki T,Pateraki I,Bosabalidis AM,Kafetzopoulos D,Demetzos C,Pichersky E,Kanellis AK

更新日期：2008-12-01 00:00:00

abstract：:GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...

journal_title：Plant molecular biology

authors： Gu L,Zhang Y,Zhang M,Li T,Dirk LM,Downie B,Zhao T

更新日期：2016-01-01 00:00:00

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：KEY MESSAGE:We identified three dormant stages of Polygonatum kingianum and changes that occurred during dormancy transition in the following aspects including cell wall and hormones, as well as interaction among them. Polygonatum kingianum Coll.et Hemsl (P. kingianum) is an important traditional Chinese medicine, but ...

journal_title：Plant molecular biology

authors： Wang Y,Liu X,Su H,Yin S,Han C,Hao D,Dong X

更新日期：2019-02-01 00:00:00

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...

journal_title：Plant molecular biology

authors： Hamill JD,Robins RJ,Parr AJ,Evans DM,Furze JM,Rhodes MJ

更新日期：1990-07-01 00:00:00

abstract：:The often rapid and synchronous programmed death of petal cells provides a model system to study molecular aspects of organ senescence. The death of petal cells is preceded by a loss of membrane permeability, due in part to increases in reactive oxygen species that are in turn related to up-regulation of oxidative enz...

journal_title：Plant molecular biology

authors： Rubinstein B

更新日期：2000-10-01 00:00:00

abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...

journal_title：Plant molecular biology

authors： Dalla Via V,Traubenik S,Rivero C,Aguilar OM,Zanetti ME,Blanco FA

更新日期：2017-04-01 00:00:00

abstract：:To elucidate the evolutionary relationship between the Spermatophyta, Pteridophyta and Bryophyta, we cloned a fragment of chloroplast DNA from the fern Angiopteris lygodiifolia (Pteridophyta) and determined its nucleotide sequence. The fragment contained the atpB, rbcL, trnR-CCG, dedB and psaI genes. Comparisons of th...

journal_title：Plant molecular biology

authors： Yoshinaga K,Kubota Y,Ishii T,Wada K

更新日期：1992-01-01 00:00:00

abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...

journal_title：Plant molecular biology

authors： Kao CW,Bakshi M,Sherameti I,Dong S,Reichelt M,Oelmüller R,Yeh KW

更新日期：2016-12-01 00:00:00

abstract：:A CDNA encoding a 47 kDa nucleoside triphosphatase (NTPase) that is associated with the chromatin of pea nuclei has been cloned and sequenced. The translated sequence of the cDNA includes several domains predicted by known biochemical properties of the enzyme, including five motifs characteristic of the ATP-binding do...

journal_title：Plant molecular biology

authors： Hsieh HL,Tong CG,Thomas C,Roux SJ

更新日期：1996-01-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：KEY MESSAGE:This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional mem...

journal_title：Plant molecular biology

authors： Pachamuthu K,Swetha C,Basu D,Das S,Singh I,Sundar VH,Sujith TN,Shivaprasad PV

更新日期：2021-01-01 00:00:00

abstract：:A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...

journal_title：Plant molecular biology

authors： Kaplan CP,Tugal HB,Baker A

更新日期：1997-06-01 00:00:00

abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

journal_title：Plant molecular biology

authors： Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

更新日期：1999-02-01 00:00:00

abstract：:APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...

journal_title：Plant molecular biology

authors： Krishnaswamy S,Verma S,Rahman MH,Kav NN

更新日期：2011-01-01 00:00:00

abstract：:Rhodanese-domain proteins (RDPs) are widespread in plants and other organisms, but their biological roles are mostly unknown. Here we report on a novel RDP from Chlamydomonas that has a single rhodanese domain, and a predicted chloroplast transit peptide. The protein was produced in Escherichia coli with a His-tag, bu...

journal_title：Plant molecular biology

authors： Luo L,Herrin DL

更新日期：2012-07-01 00:00:00

abstract：:The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...

journal_title：Plant molecular biology

authors： Schmidt K,Heberle B,Kurrasch J,Nehls R,Stahl DJ

更新日期：2004-08-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...

journal_title：Plant molecular biology

authors： Sharma R,Tan F,Jung KH,Sharma MK,Peng Z,Ronald PC

更新日期：2011-11-01 00:00:00

abstract：:Exposure of seedlings of a chilling-sensitive variety of rice (Oryza sativa L. cv. Wasetoittu) to water stress (0.5 M mannitol, 30 min) at room temperature induced a degree of chilling resistance. No such resistance was induced by exogenous abscisic acid (ABA) application (10 microM, 60 min). Upon short-term water str...

journal_title：Plant molecular biology

authors： Takahashi R,Joshee N,Kitagawa Y

更新日期：1994-10-01 00:00:00

abstract：:Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...

journal_title：Plant molecular biology

authors： Dunlevy JD,Soole KL,Perkins MV,Dennis EG,Keyzers RA,Kalua CM,Boss PK

更新日期：2010-09-01 00:00:00

abstract：:Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...

journal_title：Plant molecular biology

authors： Nugroho ABD,Han N,Pervitasari AN,Kim DH,Kim J

更新日期：2020-03-01 00:00:00

abstract：KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...

journal_title：Plant molecular biology

authors： Wang K,Zhao X,Pang C,Zhou S,Qian X,Tang N,Yang N,Xu P,Xu X,Gao J

更新日期：2021-01-22 00:00:00

abstract：:cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...

journal_title：Plant molecular biology

authors： Salter AH,Newman BJ,Napier JA,Gray JC

更新日期：1992-11-01 00:00:00