Over-expressing a yeast ornithine decarboxylase gene in transgenic roots of Nicotiana rustica can lead to enhanced nicotine accumulation.

abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...

journal_title：Plant molecular biology

authors： Merkelbach S,Gehlen J,Denecke M,Hirsch HJ,Kreuzaler F

更新日期：1993-11-01 00:00:00

abstract：:Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...

journal_title：Plant molecular biology

authors： Nakagawa A,Takahashi H,Kojima S,Sato N,Ohga K,Cha BY,Woo JT,Nagai K,Horiguchi G,Tsukaya H,Machida Y,Machida C

更新日期：2012-08-01 00:00:00

abstract：:We investigated the spatial and temporal expression patterns of two rice calcium-dependent protein kinases (CDPKs), OsCDPK2 and OSCDPK11, using isoform-specific antisera. Bands of the expected molecular sizes for OsCDPK2 (59 kDa) and OsCDPK11 (61 kDa) were detected on western blots. OsCDPK2 and OsCDPK11 mRNA and prote...

journal_title：Plant molecular biology

authors： Frattini M,Morello L,Breviario D

更新日期：1999-12-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:Chimaeric PCaMV35Scry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cry IA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indic...

journal_title：Plant molecular biology

authors： van Aarssen R,Soetaert P,Stam M,Dockx J,Gosselé V,Seurinck J,Reynaerts A,Cornelissen M

更新日期：1995-06-01 00:00:00

abstract：:The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...

journal_title：Plant molecular biology

authors： Huss B,Tinland B,Paulus F,Walter B,Otten L

更新日期：1990-02-01 00:00:00

abstract：:Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...

journal_title：Plant molecular biology

authors： Kinkema M,Geijskes RJ,Shand K,Coleman HD,De Lucca PC,Palupe A,Harrison MD,Jepson I,Dale JL,Sainz MB

更新日期：2014-03-01 00:00:00

abstract：:The amounts of a 1.9 kb mitochondrial plasmid relative to sequences in another mitochondrial DNA replicon and also to nuclear ribosomal DNA sequences have been compared in maize leaves and anthers. Similar comparisons have been made between plants with the same nuclear genotype but containing normal, S, or T cytoplasm...

journal_title：Plant molecular biology

authors： Abbott AG,O'Dell M,Flavell RB

更新日期：1985-07-01 00:00:00

abstract：:RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in g...

journal_title：Plant molecular biology

authors： Tseng CC,Lee CJ,Chung YT,Sung TY,Hsieh MH

更新日期：2013-07-01 00:00:00

abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

journal_title：Plant molecular biology

authors： Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

更新日期：2000-12-01 00:00:00

abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

journal_title：Plant molecular biology

authors： Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

更新日期：2020-01-01 00:00:00

abstract：:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...

journal_title：Plant molecular biology

authors： Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

更新日期：2006-05-01 00:00:00

abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...

journal_title：Plant molecular biology

authors： Latvala-Kilby SM,Kilby NJ

更新日期：2006-05-01 00:00:00

abstract：:We report the isolation of the second member, kin2, of a family of two cold-inducible genes of Arabidopsis thaliana. The proteins corresponding to the two genes have similarities to the small antifreeze proteins from Winter flounder. Kin1 and kin2 are organized in a close tandem array in the genome of A. thaliana. Bot...

journal_title：Plant molecular biology

authors： Kurkela S,Borg-Franck M

更新日期：1992-07-01 00:00:00

abstract：:We investigated the copy number of the gene for alternative oxidase (AOX) of Arabidopsis thaliana by amplification by PCR and Southern hybridization. These studies indicated that there are at least four copies of the AOX gene in Arabidopsis. We isolated genomic clones containing individual copies (designated as AOX1a,...

journal_title：Plant molecular biology

authors： Saisho D,Nambara E,Naito S,Tsutsumi N,Hirai A,Nakazono M

更新日期：1997-11-01 00:00:00

abstract：:It has been known for more than a decade that increasing the gene copy number does not necessarily lead to increased gene activity. Plants have developed efficient mechanisms such as post-transcriptional gene silencing (PTGS) to regulate abnormal gene expression in a sequence-specific fashion. PTGS of (trans)genes can...

journal_title：Plant molecular biology

authors： Morel JB,Vaucheret H

更新日期：2000-06-01 00:00:00

abstract：:The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...

journal_title：Plant molecular biology

authors： Gepstein S,Glick BR

更新日期：2013-08-01 00:00:00

abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

journal_title：Plant molecular biology

authors： Hsieh MH,Chang CY,Hsu SJ,Chen JJ

更新日期：2008-04-01 00:00:00

abstract：:Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue...

journal_title：Plant molecular biology

authors： van der Steege G,Nieboer M,Swaving J,Tempelaar MJ

更新日期：1992-10-01 00:00:00

abstract：KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron...

journal_title：Plant molecular biology

authors： Rose AB,Carter A,Korf I,Kojima N

更新日期：2016-10-01 00:00:00

abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...

journal_title：Plant molecular biology

authors： Majira A,Domin M,Grandjean O,Gofron K,Houba-Hérin N

更新日期：2002-10-01 00:00:00

abstract：:Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...

journal_title：Plant molecular biology

authors： Gray J,Picton S,Shabbeer J,Schuch W,Grierson D

更新日期：1992-05-01 00:00:00

abstract：:In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...

journal_title：Plant molecular biology

authors： Steinkellner H,Fluch S,Turetschek E,Lexer C,Streiff R,Kremer A,Burg K,Glössl J

更新日期：1997-04-01 00:00:00

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00

abstract：:The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...

journal_title：Plant molecular biology

authors： Quigley F,Grienenberger JM,Weil JH

更新日期：1985-09-01 00:00:00

abstract：:Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...

journal_title：Plant molecular biology

authors： Newton SS,Duman JG

更新日期：2000-11-01 00:00:00

abstract：:The multiple copies of the chloroplast genome (plastome) are condensed and organized into nucleoids by a set of proteins. One of these, the DNA-binding protein DCP68 from soybean, has previously been shown to compact DNA and to inhibit DNA synthesis in vitro. N-terminal amino acid analysis and the absorption spectrum ...

journal_title：Plant molecular biology

authors： Chi-Ham CL,Keaton MA,Cannon GC,Heinhorst S

更新日期：2002-08-01 00:00:00

abstract：:Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...

journal_title：Plant molecular biology

authors： Mahfouz MM,Li L,Piatek M,Fang X,Mansour H,Bangarusamy DK,Zhu JK

更新日期：2012-02-01 00:00:00