cry IA(b) transcript formation in tobacco is inefficient.

Abstract:

:Chimaeric PCaMV35Scry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cry IA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indicate that the frequency of transcription initiation is not limiting. However, the cry precursor mRNA carries at least three regions that are recognized as introns. The absence of high cytoplasmic levels of spliced cry mRNAs suggests that these mRNAs are unstable and/or not efficiently made. Point mutations in the 5' splice site of the most distal intron allows high accumulation levels of the full-length mRNA. This implies that the inefficient formation of full-size mRNA is a major cause of the low expression level of chimaeric cry IA(b) genes in tobacco.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

van Aarssen R,Soetaert P,Stam M,Dockx J,Gosselé V,Seurinck J,Reynaerts A,Cornelissen M

doi

10.1007/BF00020398

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

513-24

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

28

pub_type

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