Abstract:
:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-dehydroquinate synthase-deficient Escherichia coli strain with a tomato cDNA library. The deduced amino acid sequence contains a putative N-terminal plastid-specific transit peptide, and the sequence of the mature enzyme resembles those of the corresponding bacterial enzymes more than of the fungal enzymes. Sequence identity was even higher between the tomato and E. coli sequences than between the E. coli and other known bacterial sequences. The abundance of 3-dehydroquinate synthase transcripts differ in the organs of tomato plants analyzed. In cultured tomato cells, the abundance of 3-dehydroquinate synthase transcripts increased 9-fold within 4 to 5 h of elicitor treatment.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid Jdoi
10.1007/BF00020607subject
Has Abstractpub_date
1996-04-01 00:00:00pages
69-76issue
1eissn
0167-4412issn
1573-5028journal_volume
31pub_type
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