Abstract：
KEY MESSAGE:Related motifs strongly increase gene expression when added to an intron located in coding sequences. Many introns greatly increase gene expression through a mechanism that remains elusive. An obstacle to understanding intron-mediated enhancement (IME) has been the difficulty of locating the specific intron sequences responsible for boosting expression because they are redundant, dispersed, and degenerate. Previously we used the IMEter algorithm in two independent ways to identify two motifs (CGATT and TTNGATYTG) that are candidates for involvement in IME in Arabidopsis. Here we show that both motifs are sufficient to increase expression. An intron that has little influence on expression was converted into one that increased mRNA accumulation 24-fold and reporter enzyme activity 40-fold relative to the intronless control by introducing 11 copies of the more active TTNGATYTG motif. This degree of stimulation is twice as large as that of the strongest of 15 natural introns previously tested in the same reporter gene. Even though the CGATT and TTNGATYTG motifs each increased expression, and CGATT matches the NGATY core of the longer motif, combining the motifs to make TTCGATTTG reduced the stimulating ability of the TTNGATYTG motif. Additional substitutions were used to test the contribution to IME of other residues in the TTNGATYTG motif. The verification that these motifs are active in IME will improve our ability to predict the stimulating ability of introns, to engineer any intron to increase expression to a desired level, and to explore the mechanism of IME by seeking factors that might interact with these sequences.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Rose AB,Carter A,Korf I,Kojima Ndoi
10.1007/s11103-016-0516-1subject
Has Abstractpub_date
2016-10-01 00:00:00pages
337-46issue
3eissn
0167-4412issn
1573-5028pii
10.1007/s11103-016-0516-1journal_volume
92pub_type
杂志文章abstract：:The PR-like proteins, class I beta-1,3-glucanase (GLU I) and chitinase (CHN I), are induced as part of a stereotypic response that can provide protection against viral, bacterial, and fungal pathogens. We have identified two Nicotiana plumbaginifolia ankyrin-repeat proteins, designated Glucanohydrolase Binding Protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-4679-9
更新日期：2004-11-01 00:00:00
abstract：:We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006375123496
更新日期：1999-11-01 00:00:00
abstract：:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019948
更新日期：1993-01-01 00:00:00
abstract：:The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040137
更新日期：1986-03-01 00:00:00
abstract：:Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9849-y
更新日期：2012-01-01 00:00:00
abstract：:The aminoglycoside antibiotic hygromycin B (Hyg) inhibits prokaryotic, chloroplast and mitochondrial protein synthesis. Because of the toxic effect of Hyg on plant cells, the HPT gene, encoding hygromycin phosphotransferase, has become one of the most widely used selectable markers in plant transformation. Yet the mec...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0380-4
更新日期：2015-12-01 00:00:00
abstract：:Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9334-4
更新日期：2008-07-01 00:00:00
abstract：:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0459-6
更新日期：2016-06-01 00:00:00
abstract：:Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027335
更新日期：1989-07-01 00:00:00
abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0531-2
更新日期：2016-12-01 00:00:00
abstract：:In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016071
更新日期：1991-06-01 00:00:00
abstract：:Plants count on a wide variety of metabolic, physiological, and developmental responses to adapt their growth to variations in mineral nutrient availability. To react to such variations plants have evolved complex sensing and signaling mechanisms that allow them to monitor the external and internal concentration of ea...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-008-9380-y
更新日期：2009-03-01 00:00:00
abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9441-2
更新日期：2009-03-01 00:00:00
abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014447
更新日期：1994-03-01 00:00:00
abstract：:GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9345-1
更新日期：2008-08-01 00:00:00
abstract：:Proline-rich protein (PRP), a cell wall protein of plant, has been studied in many plant species. Yet, none of the PRPs has been functionally elucidated. Here we report a novel flower-specific PRP designated OsPRP3 from rice. Expression analysis showed that the OsPRP3 transcript was mainly present in rice flower and a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9557-z
更新日期：2010-01-01 00:00:00
abstract：:Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (T...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-2619-y
更新日期：2005-12-01 00:00:00
abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005790927581
更新日期：1997-03-01 00:00:00
abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00033384
更新日期：1984-11-01 00:00:00
abstract：:We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separat...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00026782
更新日期：1992-07-01 00:00:00
abstract：:Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028803
更新日期：1993-02-01 00:00:00
abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006232700835
更新日期：1999-09-01 00:00:00
abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019477
更新日期：1996-07-01 00:00:00
abstract：:Osmium tetroxide (OsO4) reacts with the thymine residues of double-stranded DNA, but thymines that are unpaired or under torsional stress are hyperreactive. Although OsO4 hyperreactivity has been primarily utilized to identify Z-DNA structures in supercoiled plasmids, OsO4 will also identify other torsional perturbati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028983
更新日期：1993-09-01 00:00:00
abstract：:TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00024193
更新日期：1994-04-01 00:00:00
abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9984-0
更新日期：2013-01-01 00:00:00
abstract：:The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0240-7
更新日期：2014-11-01 00:00:00
abstract：:Higher plant microspores, when subjected to various stress treatments in vitro, are able to reprogram their regular gametophytic development towards the sporophytic pathway to form haploid embryos and plants. Suppression subtractive hybridization (SSH) and metabolic profiling were used to characterize this development...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9078-y
更新日期：2007-01-01 00:00:00
abstract：KEY MESSAGE:Plant bioregulators play an important role in managing oxidative stress tolerance in plants. Utilizing their ability in stress sensitive crops through genetic engineering will be a meaningful approach to manage food production under the threat of climate change. Exploitation of the plant defense system agai...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-020-01077-w
更新日期：2021-01-01 00:00:00
abstract：:A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016515
更新日期：1990-05-01 00:00:00
