Abstract:
:We report the isolation and characterisation of a wound-induced cDNA designated AoPR1 from a suspension of mesophyll cells that had been mechanically isolated from cladodes of light-grown Asparagus officinalis seedlings by grinding in a mortar and pestle. The transcript abundance is up-regulated following cell separation and in chopped mesocotyl tissue from dark-grown seedlings. The expression of AoPR1 was shown by northern analysis to be located around the site of damage. Sequence analysis revealed similarity between the predicted AoPR1 polypeptide and bean PvPR1 and PvPR2 proteins, the potato pSTH2 protein, the pea PI49 protein, the parsley PcPR1-1 protein and a major pollen allergen from birch (BetvI). These transcripts have been shown to be induced in response to microbial attack or fungal elicitation. To our knowledge, this is the first example of a monocot cDNA belonging to this class of intracellular pathogenesis-related proteins (IPRs).
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Warner SA,Scott R,Draper Jdoi
10.1007/BF00026782subject
Has Abstractpub_date
1992-07-01 00:00:00pages
555-61issue
4eissn
0167-4412issn
1573-5028journal_volume
19pub_type
杂志文章abstract::Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006496308160
更新日期:2000-04-01 00:00:00
abstract::We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028815
更新日期:1993-02-01 00:00:00
abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006487326533
更新日期:2001-01-01 00:00:00
abstract::The suppression of plant defence reactions plays a crucial role in causing plant diseases. In this report, we show that inducible plant defences are repressed during the development of Cercospora leaf spot disease. In the early phase of infection of sugar beet (Beta vulgaris L.) leaves with the phytopathogenic fungus ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-2141-7
更新日期:2004-08-01 00:00:00
abstract::A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00034937
更新日期:1986-09-01 00:00:00
abstract::Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are, however, still largely unknown. We applied a transcriptomic approach to a common member of the genus, T. gelatinosa, to investigate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0468-5
更新日期:2016-06-01 00:00:00
abstract:KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00848-4
更新日期:2019-05-01 00:00:00
abstract::RNA b is the most abundant member of a family of autonomously replicating single- and double-stranded RNA plasmids found in maize mitochondria. The extent to which this molecule is associated with proteins was investigated by rate zonal and CsCl equilibrium density gradient centrifugation of clarified lysates of S cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005818631401
更新日期:1997-06-01 00:00:00
abstract::Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction protein, the chloroplast co-chaperonin 20 (CPN20) in ABA signaling in Arabidopsis thaliana. We showed that down-e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0082-8
更新日期:2013-10-01 00:00:00
abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
abstract::A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023388
更新日期:1992-06-01 00:00:00
abstract::Plants respond to phosphate (Pi) deficiency through adaptive mechanisms involving several morphological, biochemical and molecular changes. In this study, we have characterized the structure and expression of a tomato (Lycopersicon esculentum L.) phosphate starvation-induced gene (TPSI1). A 3.5 kb genomic fragment con...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005729309569
更新日期:1997-03-01 00:00:00
abstract::A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020881
更新日期:1995-03-01 00:00:00
abstract::A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00033384
更新日期:1984-11-01 00:00:00
abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005790927581
更新日期:1997-03-01 00:00:00
abstract::In the wake of recent progress of high throughput transcriptome profiling technologies, extensive housekeeping gene mining has been conducted in humans. However, very few studies have been reported in maize (Zea mays L.), an important crop plant, and none were conducted on a genome -wide level. In this study, we surve...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0246-1
更新日期:2014-11-01 00:00:00
abstract::The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016430
更新日期:1987-01-01 00:00:00
abstract::Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7507-3
更新日期:2005-09-01 00:00:00
abstract:KEY MESSAGE:During abiotic stress low abundant amino acids are not synthesized but they accumulate due to increased protein turnover under conditions inducing carbohydrate starvation (dehydration, salt stress, darkness) and are degraded. Metabolic adaptation is crucial for abiotic stress resistance in plants, and accum...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0767-0
更新日期:2018-09-01 00:00:00
abstract::Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-0351-2
更新日期:2005-10-01 00:00:00
abstract::A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4208-5
更新日期:2006-02-01 00:00:00
abstract::Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9866-x
更新日期:2012-02-01 00:00:00
abstract:KEY MESSAGE:OsWRKY36 represses plant height and grain size by inhibiting gibberellin signaling. Plant height and grain size are important agronomic traits affecting yield in cereals, including rice. Gibberellins (GAs) are plant hormones that promote plant growth and developmental processions such as stem elongation and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01049-0
更新日期:2020-11-01 00:00:00
abstract::An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9178-3
更新日期:2007-07-01 00:00:00
abstract:KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0543-y
更新日期:2017-01-01 00:00:00
abstract::Silicon (Si) confers several benefits to many plant species when absorbed as silicic acid through nodulin 26-like intrinsic proteins (NIPs). The NIPs belong to major intrinsic protein (MIP) family, members of which form channels with high selectivity to control transport of water and different solutes. Here, comparati...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0087-3
更新日期:2013-11-01 00:00:00
abstract::Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042047
更新日期:1995-04-01 00:00:00
abstract::A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9768-y
更新日期:2011-05-01 00:00:00