Altered expression of an ankyrin-repeat protein results in leaf abnormalities, necrotic lesions, and the elaboration of a systemic signal.

abstract：:Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...

journal_title：Plant molecular biology

authors： Wu K,Malik K,Tian L,Brown D,Miki B

更新日期：2000-09-01 00:00:00

abstract：:Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...

journal_title：Plant molecular biology

authors： Gong Z,Yamazaki M,Sugiyama M,Tanaka Y,Saito K

更新日期：1997-12-01 00:00:00

abstract：:Despite the presence in nature of many functional gene families that contain several to many highly similar sequences, the presence of identical DNA sequence repeats is widely thought to predispose transgene inserts to homology dependent gene silencing (HDGS). The induction of transcriptional gene silencing (TGS) by R...

journal_title：Plant molecular biology

authors： Yang G,Lee YH,Jiang Y,Kumpatla SP,Hall TC

更新日期：2005-06-01 00:00:00

abstract：:Tree crops have a long juvenile period which is a serious constraint for genetic improvement and experimental research. For example, apple remains in a juvenile phase for more than five years after seed germination. Here, we report about induction of rapid flowering in apple seedlings using the Apple latent spherical ...

journal_title：Plant molecular biology

authors： Yamagishi N,Sasaki S,Yamagata K,Komori S,Nagase M,Wada M,Yamamoto T,Yoshikawa N

更新日期：2011-01-01 00:00:00

abstract：KEY MESSAGE:HbMBF1a was isolated and characterized in H. brevisubulatum, and overexpressed HbMBF1a could enhance the salt tolerance and ABA insensitivity in Arabidopsis thaliana. The transcript levels of stress-responsive genes were significantly increased in the transgenic lines under salt and ABA conditions. Salinity...

journal_title：Plant molecular biology

authors： Zhang L,Wang Y,Zhang Q,Jiang Y,Zhang H,Li R

更新日期：2020-01-01 00:00:00

abstract：:A highly purified RNA polymerase preparation from pea chloroplasts has been shown to specifically transcribe the 16S rRNA gene in vitro using the recombinant pCB2-8 DNA as a template. The RNA polymerase has been found to show maximum activity and specificity with pea supercoiled rDNA as a template. At low concentratio...

journal_title：Plant molecular biology

authors： Sun E,Shapiro DR,Wu BW,Tewari KK

更新日期：1986-11-01 00:00:00

abstract：:In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A m...

journal_title：Plant molecular biology

authors： Babula-Skowrońska D,Ludwików A,Cieśla A,Olejnik A,Cegielska-Taras T,Bartkowiak-Broda I,Sadowski J

更新日期：2015-07-01 00:00:00

abstract：:Two distinct cDNAs for 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) were obtained from a glyphosate-tolerant tobacco cell line. The cDNAs were 89% identical and the predicted sequences of the mature proteins were greater than 83% identical with EPSPS proteins from other plants. Tobacco EPSPS proteins were more ...

journal_title：Plant molecular biology

authors： Wang YX,Jones JD,Weller SC,Goldsbrough PB

更新日期：1991-12-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants constitutively expressing Agrobacterium tumefaciens tryptophan monooxygenase (iaaM) were obtained and characterized. Arabidopsis plants expressing iaaM have up to 4-fold higher levels of free indole-3-acetic acid (IAA) and display increased hypocotyl elongation in the light. This...

journal_title：Plant molecular biology

authors： Romano CP,Robson PR,Smith H,Estelle M,Klee H

更新日期：1995-03-01 00:00:00

abstract：:Plants, algae, cyanobacteria and many other bacteria synthesize the tetrapyrrole precursor, delta-aminolevulinic acid (ALA), from glutamate by means of a tRNAGlu-mediated pathway. The enzyme glutamyl-tRNA reductase (GTR) catalyzes the first committed step in this pathway, which is the reduction of tRNA-bound glutamate...

journal_title：Plant molecular biology

authors： Srivastava A,Lake V,Nogaj LA,Mayer SM,Willows RD,Beale SI

更新日期：2005-07-01 00:00:00

abstract：:A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...

journal_title：Plant molecular biology

authors： Man AL,Purcell PC,Hannappel U,Halford NG

更新日期：1997-05-01 00:00:00

abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...

journal_title：Plant molecular biology

authors： Hsieh WL,Wolniak SM

更新日期：1998-05-01 00:00:00

abstract：:The chloroplast psbB operon includes five genes encoding photosystem II and cytochrome b 6 /f complex components. The psbN gene is located on the opposite strand. PsbN is localized in the thylakoid and is present even in the dark, although its level increases upon illumination and then decreases. However, the translat...

journal_title：Plant molecular biology

authors： Kuroda H,Sugiura M

更新日期：2014-12-01 00:00:00

abstract：:Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...

journal_title：Plant molecular biology

authors： Mohamed A,Jansson C

更新日期：1991-05-01 00:00:00

abstract：KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...

journal_title：Plant molecular biology

authors： Xu H,Wang N,Liu J,Qu C,Wang Y,Jiang S,Lu N,Wang D,Zhang Z,Chen X

更新日期：2017-05-01 00:00:00

abstract：:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

journal_title：Plant molecular biology

authors： Lawton K,Ward E,Payne G,Moyer M,Ryals J

更新日期：1992-08-01 00:00:00

abstract：:Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...

journal_title：Plant molecular biology

authors： He Y,Gan S

更新日期：2004-01-01 00:00:00

abstract：:A certain nucleotide sequence in the promoter region of Vicia faba rRNA genes that specifically binds to a nuclear protein fraction has been identified by using a gel retardation assay and DNase I footprinting technique. The binding site of this protein fraction is located about 60 bp upstream from the initiation site...

journal_title：Plant molecular biology

authors： Nakajima T,Suzůki A,Tanifuji S,Kato A

更新日期：1992-12-01 00:00:00

abstract：:We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...

journal_title：Plant molecular biology

authors： Hildebrand M,Hasegawa P,Ord RW,Thorpe VS,Glass CA,Volcani BE

更新日期：1992-08-01 00:00:00

abstract：:Several clones corresponding to the gene encoding NADH-dependent hydroxypyruvate reductase have been isolated from a cucumber genomic library. Restriction mapping indicates the presence of two HPR genes, hpr-A and hpr-B, in the cucumber genome. Examination of the DNAs of individual plants suggests that hpr-A and hpr-B...

journal_title：Plant molecular biology

authors： Schwartz BW,Sloan JS,Becker WM

更新日期：1991-10-01 00:00:00

abstract：:Anaerobiosis rapidly induces alcohol dehydrogenase (ADH), an enzyme of the fermentation pathway, in different parts of rice seedlings. After initiation of anaerobiosis, the activity of the enzyme increases linearly for 3 days or more. The ADH activity is anaerobically inducible even in mature rice leaves in contrast t...

journal_title：Plant molecular biology

authors： Xie Y,Wu R

更新日期：1989-07-01 00:00:00

abstract：:The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...

journal_title：Plant molecular biology

authors： Urquhart W,Gunawardena AH,Moeder W,Ali R,Berkowitz GA,Yoshioka K

更新日期：2007-12-01 00:00:00

abstract：:Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...

journal_title：Plant molecular biology

authors： Burrell MM,Temple S,Ooms G

更新日期：1986-07-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Clones encoding beta-1,3-glucanase have been isolated from a Hevea cDNA library prepared from the latex of Hevea brasiliensis using a probe Nicotiana plumbaginifolia cDNA encoding beta-1,3-glucanase, gnl. Nucleotide sequence analysis showed that a 1.2 kb Hevea cDNA encoding a basic beta-1,3-glucanase showed 68% nucleo...

journal_title：Plant molecular biology

authors： Chye ML,Cheung KY

更新日期：1995-10-01 00:00:00

abstract：:We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...

journal_title：Plant molecular biology

authors： Rathore KS,Chowdhury VK,Hodges TK

更新日期：1993-03-01 00:00:00

abstract：:The ENOD40 gene is induced early during Rhizobium-legume symbiosis and has probably a primary role in the nodule organogenesis. In this paper we show that the 1.7 kb 5'-flanking region of the GmENOD40(2) is able to drive the expression of a gusA-int marker in transgenic Arabidopsis thaliana. The promoter activity is d...

journal_title：Plant molecular biology

authors： Mirabella R,Martirani L,Lamberti A,Iaccarino M,Chiurazzi M

更新日期：1999-01-01 00:00:00

abstract：:cDNA clones were isolated and sequenced that encode two related but distinct rice cyclophilins, Cyp1 and Cyp2. The predicted amino acid sequences of each are 72% identical to human T-cell cyclophilin. Genomic DNA gel blot analysis suggests cyclophilins in rice are encoded by a small, 6-10-member gene family. Both Cyp1...

journal_title：Plant molecular biology

authors： Buchholz WG,Harris-Haller L,DeRose RT,Hall TC

更新日期：1994-08-01 00:00:00

abstract：:Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

journal_title：Plant molecular biology

authors： Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

更新日期：2003-09-01 00:00:00

abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

journal_title：Plant molecular biology

authors： Sakamoto K,Nagatani A

更新日期：1996-08-01 00:00:00