Abstract:
:In this report we characterized the Arabidopsis ABI1 gene orthologue and Brassica napus gene paralogues encoding protein phosphatase 2C (PP2C, group A), which is known to be a negative regulator of the ABA signaling pathway. Six homologous B. napus sequences were identified and characterized as putative PP2C group A members. To gain insight into the conservation of ABI1 function in Brassicaceae, and understand better its regulatory effects in the drought stress response, we generated transgenic B. napus plants overexpressing A. thaliana ABI1. Transgenic plants subjected to drought showed a decrease in relative water content, photosynthetic pigments content and expression level of RAB18- and RD19A-drought-responsive marker genes relative to WT plants. We present the characterization of the drought response of B. napus with the participation of ABI1-like paralogues. The expression pattern of two evolutionarily distant paralogues, BnaA01.ABI1.a and BnaC07.ABI1.b in B. napus and their promoter activity in A. thaliana showed differences in the induction of the paralogues under dehydration stress. Comparative sequence analysis of both BnaABI1 promoters showed variation in positions of cis-acting elements that are especially important for ABA- and stress-inducible expression. Together, these data reveal that subfunctionalization following gene duplication may be important in the maintenance and functional divergence of the BnaABI1 paralogues. Our results provide a framework for a better understanding of (1) the role of ABI1 as a hub protein regulator of the drought response, and (2) the differential involvement of the duplicated BnaABI1 genes in the response of B. napus to dehydration-related stresses.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Babula-Skowrońska D,Ludwików A,Cieśla A,Olejnik A,Cegielska-Taras T,Bartkowiak-Broda I,Sadowski Jdoi
10.1007/s11103-015-0334-xsubject
Has Abstractpub_date
2015-07-01 00:00:00pages
445-57issue
4-5eissn
0167-4412issn
1573-5028journal_volume
88pub_type
杂志文章abstract:KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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更新日期:2019-07-01 00:00:00
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journal_title:Plant molecular biology
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