Abstract:
:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent molecular-genetic model to study the biosynthesis and modification of cell wall components, particularly pectin. To support such research, we sought to identify a promoter that drives expression specifically in the Arabidopsis seed coat epidermis. Arabidopsis seed coat microarray data was analysed for genes expressed in the wild type seed coat but not the seed coat of the apetala2 mutant where the epidermal cells fail to differentiate. Of 14 candidate genes, 9 showed a seed-specific expression pattern by reverse transcriptase-PCR. Transcriptional regulatory region-β-glucuronidase (GUS) reporter gene fusions introduced into Arabidopsis identified one promoter, that of the DIRIGENT PROTEIN1 (DP1) gene, as seed coat specific. The specificity of the expression was confirmed using a second reporter gene, Citrine YFP. Expression of both reporter genes was limited to the epidermal and palisade cell layers of the seed coat. Quantitative PCR data using wild type seed coat RNA suggested that the promoter is particularly active at 7 days post anthesis. The DP1 promoter was able to direct transcription of GUS in a similar pattern in the Brassica napus seed coat. Thus, in addition to its application in studying the plant cell wall, this promoter will provide an experimental tool for expressing high-valued recombinant proteins as well as modifying seed coat traits in economically important crops.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Esfandiari E,Jin Z,Abdeen A,Griffiths JS,Western TL,Haughn GWdoi
10.1007/s11103-012-9984-0subject
Has Abstractpub_date
2013-01-01 00:00:00pages
93-104issue
1-2eissn
0167-4412issn
1573-5028journal_volume
81pub_type
杂志文章abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020803
更新日期:1996-03-01 00:00:00
abstract:KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0563-7
更新日期:2017-02-01 00:00:00
abstract::To understand the transcriptional responses to flooding stress in roots including hypocotyl of soybean seedlings, genome-wide changes in gene expression were analyzed using a soybean microarray chip containing 42,034 60-mer oligonucleotide probes. More than 6,000 of flooding-responsive genes in the roots including hyp...
journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
abstract::Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0460-0
更新日期:2016-06-01 00:00:00
abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040831
更新日期:1996-09-01 00:00:00
abstract::In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1006438321386
更新日期:2000-08-01 00:00:00
abstract::The expression of Brazil nut storage albumin genes is highly regulated during seed development. Several sequences in the promoter of one of these genes show homologies with the target sites of the maize O2 bZIP regulatory protein. We therefore asked whether the O2 protein would recognize these promoter sequences. We s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005874404706
更新日期:1997-08-01 00:00:00
abstract::An approximately 247-kb genomic region from FF genome of wild rice Oryza brachyantha, possessing the smallest Oryza genome, was compared to the orthologous approximately 450-kb region from AA genome, O. sativa L. ssp. japonica. 37 of 38 genes in the orthologous regions are shared between japonica and O. brachyantha. A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9178-3
更新日期:2007-07-01 00:00:00
abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0526-z
更新日期:2016-11-01 00:00:00
abstract::A cDNA containing the entire coding region for the iron storage protein ferritin has been isolated from the French bean plant, Phaseolus vulgaris L. cv. Tendergreen. Ferritin protein was purified from young leaves and shoot meristem tissue and used to raise antisera in mice. A lambda gt11 cDNA library was constructed ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040644
更新日期:1991-09-01 00:00:00
abstract::We present evidence for a unique covalent modification of a nuclear-encoded precursor protein targeted to plant mitochondria. We investigated the early events of in vitro import for the mitochondrial precursor of the ATP synthase F1beta subunit from Nicotiana plumbaginifolia (pF1beta) into plant mitochondria. When pF1...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006375123496
更新日期:1999-11-01 00:00:00
abstract::We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027072
更新日期:1992-08-01 00:00:00
abstract::Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019126
更新日期:1995-10-01 00:00:00
abstract::Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9866-x
更新日期:2012-02-01 00:00:00
abstract::HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022324920316
更新日期:2003-03-01 00:00:00
abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015227
更新日期:1986-07-01 00:00:00
abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0075-7
更新日期:2013-07-01 00:00:00
abstract:KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-019-00945-4
更新日期:2020-02-01 00:00:00
abstract::To play an essential role in C4 photosynthesis, the maize C4 phosphoenolpyruvate carboxylase gene (PPCZm1) acquired many new expression features, such as leaf specificity, mesophyll specificity, light inducibility and high activity, that distinguish the unique C4 PPC from numerous non-C4 PPC genes in maize. We present...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006487326533
更新日期:2001-01-01 00:00:00
abstract::The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0395-x
更新日期:2016-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
abstract::Alignment of transcripts and genome sequences yielded a set of alternatively spliced transcripts in four angiosperm genomes: three dicotyledon species Medicago truncatula (Medicago), Populus trichocarpa (poplar) and Arabidopsis thaliana (Arabidopsis), and the monocotyledon Oryzae sativa (rice). Intron retention was th...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9334-4
更新日期:2008-07-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract::R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0773-2
更新日期:2018-09-01 00:00:00
abstract::The ubiquitous grapevine-associated octopine/cucumopine Ti plasmids of biotype III Agrobacterium tumefaciens strains carry two T regions, TA and TB, with a complex oncogene arrangement. Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL re...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00018558
更新日期:1990-02-01 00:00:00
abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020254
更新日期:1995-05-01 00:00:00
abstract::A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9698-0
更新日期:2010-12-01 00:00:00
abstract::Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-5817-8
更新日期:2006-05-01 00:00:00
abstract::A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016504
更新日期:1990-05-01 00:00:00
abstract::We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000023670.61059.1d
更新日期:2003-12-01 00:00:00