Abstract:
:Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three species. The level of glycinebetaine in A. marina increased under high salinity. Betaine-aldehyde dehydrogenase (BADH) was detected in all four species, but choline monooxygenase could not be detected. A cDNA library was constructed from the leaves of A. marina. Two kinds of BADH cDNAs were isolated, one homologous to the spinach chloroplast BADH, and the other with unique residues SKL at the end of C-terminus. The BADH transcription levels of the former were higher than those of the latter. The levels of the former BADH increased at high salinity whereas those of the latter were independent of salinity. BADHs were expressed in Escherichia coli and purified. Two kinds of A. marina BADHs exhibited similar kinetic and stability properties, but were significantly different from those of spinach BADH. A. marina BADHs efficiently catalyzed the oxidation of betainealdehyde, but not the oxidation of omega-aminoaldehydes and were more stable at high temperature than the spinach BADH.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Hibino T,Meng YL,Kawamitsu Y,Uehara N,Matsuda N,Tanaka Y,Ishikawa H,Baba S,Takabe T,Wada K,Ishii T,Takabe Tdoi
10.1023/a:1006497113323subject
Has Abstractpub_date
2001-02-01 00:00:00pages
353-63issue
3eissn
0167-4412issn
1573-5028journal_volume
45pub_type
杂志文章abstract::The presence of specific glycine-rich proteins (GRP) related to petunia GRP1 (ptGRP1) was examined in three species of monocots (wheat, barley and maize) and five species of dicots (rape, turnip, soybean, crabapple and tomato). Protein blot analysis showed that anti-ptGRP1 antibody cross-reacted with a single differen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020616
更新日期:1996-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000040900.61345.a6
更新日期:2004-03-01 00:00:00
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039553
更新日期:1994-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2020-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005839832096
更新日期:1997-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028983
更新日期:1993-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0246-1
更新日期:2014-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0334-x
更新日期:2015-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9125-8
更新日期:2007-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027312
更新日期:1989-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019399
更新日期:1990-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027495
更新日期:1990-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-4208-5
更新日期:2006-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1024822312038
更新日期:2003-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1995-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-05-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028878
更新日期:1994-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019093
更新日期:1996-11-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014544
更新日期:1993-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023564
更新日期:1994-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2011-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/BF00016474
更新日期:1994-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9345-1
更新日期:2008-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01018-7
更新日期:2020-08-01 00:00:00