The b-32 protein from maize endosperm: characterization of genomic sequences encoding two alternative central domains.

abstract：:Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...

journal_title：Plant molecular biology

authors： Joshi CP,Nguyen HT

更新日期：1996-06-01 00:00:00

abstract：:The intrinsic chlorophyll-protein CP 47 is a component of photosystem II which functions in both light-harvesting and oxygen evolution. The large extrinsic loop E of this protein has been shown to interact with the oxygen-evolving site. Previously, Vermaas and coworkers have produced a number of deletions within loop ...

journal_title：Plant molecular biology

authors： Putnam-Evans C,Wu J,Bricker TM

更新日期：1996-12-01 00:00:00

abstract：:Transformed root cultures of Nicotiana rustica have been generated in which the gene from the yeast Saccharomyces cerevisiae coding for ornithine decarboxylase has been integrated. The gene, driven by the powerful CaMV35S promoter with an upstream duplicated enhancer sequence, shows constitutive expression throughout ...

journal_title：Plant molecular biology

authors： Hamill JD,Robins RJ,Parr AJ,Evans DM,Furze JM,Rhodes MJ

更新日期：1990-07-01 00:00:00

abstract：:Gibberellin (GA) 20-oxidase (GA20ox) is a key enzyme that normally catalyzes the penultimate steps in GA biosynthesis. One of the GA20ox genes in rice (Oryza sativa L.), OsGA20ox2 ( SD1 ), is well known as the "Green Revolution gene", and loss-of function mutation in this locus causes semi-dwarfism. Another GA20ox gen...

journal_title：Plant molecular biology

authors： Oikawa T,Koshioka M,Kojima K,Yoshida H,Kawata M

更新日期：2004-07-01 00:00:00

abstract：:Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

journal_title：Plant molecular biology

authors： Ni Z,Hu Z,Jiang Q,Zhang H

更新日期：2013-05-01 00:00:00

abstract：:A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

journal_title：Plant molecular biology

authors： Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

更新日期：1997-10-01 00:00:00

abstract：:A method (termed co-cultivation) for transforming plant cells in vitro with A. tumefaciens strains, which was originally developed by Marton et al. (1978) Nature 277: 129-131, has been modified by the incorporation of a novel feeder plate culture system and been extended to use with petunia protoplasts. Using efficien...

journal_title：Plant molecular biology

authors： Fraley RT,Horsch RB,Matzke A,Chilton MD,Chilton WS,Sanders PR

更新日期：1984-11-01 00:00:00

abstract：KEY MESSAGE:We reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthases that control the partitioning of precursors for lavender terpenoids. Lavender essential oil is composed of regular and irregular monoterpenes, which are derived from linear precursors geranyl diphosphat...

journal_title：Plant molecular biology

authors： Adal AM,Mahmoud SS

更新日期：2020-03-01 00:00:00

abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...

journal_title：Plant molecular biology

authors： Wu TM,Lin KC,Liau WS,Chao YY,Yang LH,Chen SY,Lu CA,Hong CY

更新日期：2016-01-01 00:00:00

abstract：:Cassava mosaic disease (CMD) is a viral disease of the important tropical staple crop cassava (Manihot esculenta) and preferred management involves use of host-plant resistance. The best available resistance is controlled by a single dominant gene. Serial analysis of gene expression (SAGE) was used to analyze the gene...

journal_title：Plant molecular biology

authors： Fregene M,Matsumura H,Akano A,Dixon A,Terauchi R

更新日期：2004-11-01 00:00:00

abstract：:The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the who...

journal_title：Plant molecular biology

authors： Suoniemi A,Anamthawat-Jónsson K,Arna T,Schulman AH

更新日期：1996-03-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...

journal_title：Plant molecular biology

authors： Tetreault HM,Gries T,Palmer NA,Funnell-Harris DL,Sato S,Ge Z,Sarath G,Sattler SE

更新日期：2020-06-01 00:00:00

abstract：:Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...

journal_title：Plant molecular biology

authors： Schwartzbach SD,Osafune T,Löffelhardt W

更新日期：1998-09-01 00:00:00

abstract：:In an attempt to identify domains directly involved in the signal transduction of phytochrome B (phyB), we over-expressed the achromophoric C-terminal half of phyB under control of the CaMV-35S promoter in transgenic Arabidopsis. In three independent transgenic lines, we detected accumulation of the introduced protein...

journal_title：Plant molecular biology

authors： Sakamoto K,Nagatani A

更新日期：1996-08-01 00:00:00

abstract：:RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

journal_title：Plant molecular biology

authors： Tang F,Yang S,Gao M,Zhu H

更新日期：2013-07-01 00:00:00

abstract：:Chemically inducible gene switches can provide precise control over gene expression, enabling more specific analyses of gene function and expanding the plant biotechnology toolkit beyond traditional constitutive expression systems. The alc gene expression system is one of the most promising chemically inducible gene s...

journal_title：Plant molecular biology

authors： Kinkema M,Geijskes RJ,Shand K,Coleman HD,De Lucca PC,Palupe A,Harrison MD,Jepson I,Dale JL,Sainz MB

更新日期：2014-03-01 00:00:00

abstract：:A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...

journal_title：Plant molecular biology

authors： Kojima K,Yamamoto N,Sasaki S

更新日期：1992-06-01 00:00:00

abstract：:A cDNA encoding the multifunctional cytochrome P450, CYP71E1, involved in the biosynthesis of the cyanogenic glucoside dhurrin from Sorghum bicolor (L.) Moench was isolated. A PCR approach based on three consensus sequences of A-type cytochromes P450- (V/I)KEX(L/F)R, FXPERF, and PFGXGRRXCXG-was applied. Three novel cy...

journal_title：Plant molecular biology

authors： Bak S,Kahn RA,Nielsen HL,Moller BL,Halkier BA

更新日期：1998-02-01 00:00:00

abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

journal_title：Plant molecular biology

authors： Atanassova R,Flénet M,Gigot C,Chaubet N

更新日期：1998-05-01 00:00:00

abstract：KEY MESSAGE:The OsPLS2 locus was isolated and cloned by map-based cloning that encodes a Upf1-like helicase. Disruption of OsPLS2 accelerated light-dependent leaf senescence in the rice mutant of ospls2. Leaf senescence is a very complex physiological process controlled by both genetic and environmental factors, howeve...

journal_title：Plant molecular biology

authors： Gong P,Luo Y,Huang F,Chen Y,Zhao C,Wu X,Li K,Yang X,Cheng F,Xiang X,Wu C,Pan G

更新日期：2019-05-01 00:00:00

abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...

journal_title：Plant molecular biology

authors： Hsieh WL,Wolniak SM

更新日期：1998-05-01 00:00:00

abstract：:14-3-3 proteins function as regulators of a wide range of target proteins in all eukaryotes by effecting direct protein-protein interactions. Primarily, interactions between 14-3-3 proteins and their targets are mediated by phosphorylation at specific sites on the target protein. Hence, interactions with 14-3-3s are s...

journal_title：Plant molecular biology

authors： Roberts MR,Salinas J,Collinge DB

更新日期：2002-12-01 00:00:00

abstract：KEY MESSAGE:Leveraging the heightened levels of polymorphism in NB-ARC-related protein encoding genes in higher plants, a bioinformatic pipeline was created to identify regions in this gene family from sequenced plant genomes that exhibit fragment length or single nucleotide differences in different accessions of the s...

journal_title：Plant molecular biology

authors： Chu P,Wilson GM,Michael TP,Vaiciunas J,Honig J,Lam E

更新日期：2018-10-01 00:00:00

abstract：:We have previously shown that mRNA and protein encoded by the Pvlea-18 gene from Phaseolus vulgaris L., a member of a new family of late embryogenesis-abundant (LEA) proteins, accumulate in dark-grown bean seedlings not only in response to water deficit but also during optimal irrigation. In this work, we studied Pvle...

journal_title：Plant molecular biology

authors： Moreno-Fonseca LP,Covarrubias AA

更新日期：2001-03-01 00:00:00

abstract：:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

journal_title：Plant molecular biology

authors： van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

更新日期：1994-10-01 00:00:00

abstract：:Paramutation is a heritable change in gene expression induced by allele interactions. This review summarizes key experiments on three maize loci, which undergo paramutation. Similarities and differences between the phenomenology at the three loci are described. In spite of many differences with respect to the stabilit...

journal_title：Plant molecular biology

authors： Chandler VL,Eggleston WB,Dorweiler JE

更新日期：2000-06-01 00:00:00

abstract：KEY MESSAGE:To explore the function of Dof transcription factors during kernel development in maize, we first identified Dof genes in the maize genome. We found that ZmDof3 was exclusively expressed in the endosperm of maize kernel and had the features of a Dof transcription factor. Suppression of ZmDof3 resulted in a ...

journal_title：Plant molecular biology

authors： Qi X,Li S,Zhu Y,Zhao Q,Zhu D,Yu J

更新日期：2017-01-01 00:00:00

abstract：:In mammalian cells the product of the human retinoblastoma tumour suppressor gene (pRb) can recruit Rpd3-like histone deacetylases to repress transcription. In this study, we investigated whether this mechanism might also be relevant in plants and found both conserved and distinct features. The expression profiles of ...

journal_title：Plant molecular biology

authors： Rossi V,Locatelli S,Lanzanova C,Boniotti MB,Varotto S,Pipal A,Goralik-Schramel M,Lusser A,Gatz C,Gutierrez C,Motto M

更新日期：2003-02-01 00:00:00

abstract：:Rice genetic resources, comprising landrace varieties, modern and obsolete varieties, genetic stocks, breeding lines, and the wild rices, are the basis of world food security. The International Rice Genebank at the International Rice Research Institute in the Philippines conserves the largest and most diverse collecti...

journal_title：Plant molecular biology

authors： Jackson MT

更新日期：1997-09-01 00:00:00