Cloning and analysis of the gene for cystathionine gamma-synthase from Arabidopsis thaliana.

abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

journal_title：Plant molecular biology

authors： Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

更新日期：2003-01-01 00:00:00

abstract：:Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...

journal_title：Plant molecular biology

authors： Lertpiriyapong K,Sung ZR

更新日期：2003-11-01 00:00:00

abstract：:During the course of characterizing polymerase chain reaction products corresponding to protein kinases of a higher plant, Arabidopsis thaliana, we found a DNA fragment that potentially codes for a polypeptide with mosaic sequences of two classes of protein kinases, a tyrosine-specific and a serine/threonine-specific ...

journal_title：Plant molecular biology

authors： Hirayama T,Oka A

更新日期：1992-11-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：:cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...

journal_title：Plant molecular biology

authors： Bischoff M,Rösler J,Raesecke HR,Görlach J,Amrhein N,Schmid J

更新日期：1996-04-01 00:00:00

abstract：:We have used the bar gene in combination with the herbicide Basta to select transformed rice (Oryza sativa L. cv. Radon) protoplasts for the production of herbicide-resistant rice plants. Protoplasts, obtained from regenerable suspension cultures established from immature embryo callus, were transformed using PEG-medi...

journal_title：Plant molecular biology

authors： Rathore KS,Chowdhury VK,Hodges TK

更新日期：1993-03-01 00:00:00

abstract：:Approximately two-thirds of the 55 to 60 plastid ribosomal proteins are encoded in the nucleus. Since the protein products of each of these genes are needed in equal amounts for ribosome assembly, their expression may be coordinately regulated by common mechanisms. To begin to understand how the expression of these ge...

journal_title：Plant molecular biology

authors： Thompson MD,Jacks CM,Lenvik TR,Gantt JS

更新日期：1992-03-01 00:00:00

abstract：:Two different calmodulin (CaM) cDNAs (MBCaM-1 and MBCaM-2) were isolated from a vigna radiata lambda gt11 library by screening with a heterologous Arabidopsis cDNA probe (TCH-1). Both cDNAs are 85% homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by MBCaM-1 and...

journal_title：Plant molecular biology

authors： Botella JR,Arteca RN

更新日期：1994-03-01 00:00:00

abstract：:We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...

journal_title：Plant molecular biology

authors： Peracchia G,Jensen AB,Culiáñez-Macià FA,Grosset J,Goday A,Issinger OG,Pagès M

更新日期：1999-05-01 00:00:00

abstract：:Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...

journal_title：Plant molecular biology

authors： Zerjal T,Joets J,Alix K,Grandbastien MA,Tenaillon MI

更新日期：2009-09-01 00:00:00

abstract：:A seedling lethal mutant of Nicotiana plumbaginifolia (sdl-1) was isolated by transposon tagging using a maize Dissociation (Ds) element. The insertion mutation was produced by direct co-transformation of protoplasts with two plasmids: one containing Ds and a second with an Ac transposase gene. sdl-1 seedlings exhibit...

journal_title：Plant molecular biology

authors： Majira A,Domin M,Grandjean O,Gofron K,Houba-Hérin N

更新日期：2002-10-01 00:00:00

abstract：:RCT1 is a TIR-NBS-LRR-type resistance (R) gene in Medicago truncatula that confers resistance to multiple races of Colletotrichum trifolii, a hemi-biotrophic fungal pathogen that causes anthracnose disease in Medicago and other closely related legumes. RCT1 undergoes alternative splicing at both coding and 3'-untransl...

journal_title：Plant molecular biology

authors： Tang F,Yang S,Gao M,Zhu H

更新日期：2013-07-01 00:00:00

abstract：:The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...

journal_title：Plant molecular biology

authors： Pimpl P,Denecke J

更新日期：2002-12-01 00:00:00

abstract：:The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...

journal_title：Plant molecular biology

authors： Oudin A,Mahroug S,Courdavault V,Hervouet N,Zelwer C,Rodríguez-Concepción M,St-Pierre B,Burlat V

更新日期：2007-09-01 00:00:00

abstract：:Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...

journal_title：Plant molecular biology

authors： Li ZK,Fu BY,Gao YM,Xu JL,Ali J,Lafitte HR,Jiang YZ,Rey JD,Vijayakumar CH,Maghirang R,Zheng TQ,Zhu LH

更新日期：2005-09-01 00:00:00

abstract：:Agrobacterium-mediated gene transfer is the method of choice for many plant biotechnology laboratories; however, large-scale use of this organism in conifer transformation has been limited by difficult propagation of explant material, selection efficiencies and low transformation frequency. We have analyzed co-cultiva...

journal_title：Plant molecular biology

authors： Wenck AR,Quinn M,Whetten RW,Pullman G,Sederoff R

更新日期：1999-02-01 00:00:00

abstract：:The histidine auxotroph mutant his 1(-) isolated from Nicotiana plumbaginifolia haploid protoplasts was first characterized to be deficient for the enzyme histidinol phosphate aminotransferase that is responsible for one of the last steps of histidine biosynthesis. Expression of the mutated gene at the RNA level was a...

journal_title：Plant molecular biology

authors： El Malki F,Jacobs M

更新日期：2001-01-01 00:00:00

abstract：:A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...

journal_title：Plant molecular biology

authors： Chye ML,Liu CM,Tan CT

更新日期：1995-01-01 00:00:00

abstract：:We studied the early events of de novo formation of adventitious shoot meristems in stem segments of Brassica oleracea. A regeneration system was used that is efficient, rapid, highly responsive to cytokinins and does not involve callus formation, thus allowing studies on a direct developmental switch of cells in the ...

journal_title：Plant molecular biology

authors： Teo WL,Kumar P,Goh CJ,Swarup S

更新日期：2001-07-01 00:00:00

abstract：:The embryo essential gene EMB506 plays a crucial role in the transition of the Arabidopsis embryo from radial symmetry to bilateral symmetry just prior to the early heart stage of development. In addition to influencing embryo development EMB506 also affects chloroplast biogenesis. To further investigate the role of E...

journal_title：Plant molecular biology

authors： Latvala-Kilby SM,Kilby NJ

更新日期：2006-05-01 00:00:00

abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...

journal_title：Plant molecular biology

authors： Henze K,Schnarrenberger C,Kellermann J,Martin W

更新日期：1994-12-01 00:00:00

abstract：:The complete nucleotide sequence of the Chlamydomonas eugametos (Chlamydomonadales, Chlorophyceae, sensu Mattox and Stewart) mitochondrial genome has been determined (22,897 bp, 34.6% G + C). The genes identified in this circular-mapping genome include those for apocytochrome b, subunit 1 of the cytochrome oxidase com...

journal_title：Plant molecular biology

authors： Denovan-Wright EM,Nedelcu AM,Lee RW

更新日期：1998-01-01 00:00:00

abstract：:The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

journal_title：Plant molecular biology

authors： Geffers R,Cerff R,Hehl R

更新日期：2000-05-01 00:00:00

abstract：:As an important osmoprotectant, glycine betaine (GB) plays an essential role in resistance to abiotic stress in a variety of organisms, including rice (Oryza sativa L.). However, GB content is too low to be detectable in rice, although rice genome possesses several orthologs coding for betaine aldehyde dehydrogenase (...

journal_title：Plant molecular biology

authors： Tang W,Sun J,Liu J,Liu F,Yan J,Gou X,Lu BR,Liu Y

更新日期：2014-11-01 00:00:00

abstract：:Genomic variation in S1 and S2 homologous sequences, defined as the S regions, were examined in mitochondrial DNAs of 12 normal cytoplasm maize lines collected in the United States. Three genomic variants were detected among the 12 cytoplasms, eight of which were identical to the Wf9 model structure. Hybridization dat...

journal_title：Plant molecular biology

authors： McNay JW,Pring DR,Lonsdale DM

更新日期：1983-07-01 00:00:00

abstract：:Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...

journal_title：Plant molecular biology

authors： Sharma R,Tan F,Jung KH,Sharma MK,Peng Z,Ronald PC

更新日期：2011-11-01 00:00:00

abstract：:A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...

journal_title：Plant molecular biology

authors： Hu W,Feng B,Ma H

更新日期：2011-05-01 00:00:00

abstract：:The expression of beta-amylase genes in rice (Oryza sativa) and its regulation by phytohormones gibberellic acid (GA) and abscisic acid (ABA) were examined. Upon germination beta-amylase is synthesized de novo in aleurone cells and (GA) is not required. Exogenous addition of GA does not enhance the beta-amylase activi...

journal_title：Plant molecular biology

authors： Wang SM,Lue WL,Eimert K,Chen J

更新日期：1996-08-01 00:00:00

abstract：:Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...

journal_title：Plant molecular biology

authors： He Y,Gan S

更新日期：2004-01-01 00:00:00

abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...

journal_title：Plant molecular biology

authors： Wu TM,Lin KC,Liau WS,Chao YY,Yang LH,Chen SY,Lu CA,Hong CY

更新日期：2016-01-01 00:00:00