Chloroplast and cytosolic triosephosphate isomerases from spinach: purification, microsequencing and cDNA cloning of the chloroplast enzyme.

abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...

journal_title：Plant molecular biology

authors： Hsieh MH,Chang CY,Hsu SJ,Chen JJ

更新日期：2008-04-01 00:00:00

abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...

journal_title：Plant molecular biology

authors： Lee K,Eggenberger AL,Banakar R,McCaw ME,Zhu H,Main M,Kang M,Gelvin SB,Wang K

更新日期：2019-03-01 00:00:00

abstract：:A cDNA library from ethephon-treated cucumber cotyledons (Cucumis sativus L. cv. Poinsett 76) was constructed. Two cDNA clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kDa cationic peroxidase that had been previously shown to be induced by...

journal_title：Plant molecular biology

authors： Morgens PH,Callahan AM,Dunn LJ,Abeles FB

更新日期：1990-05-01 00:00:00

abstract：:The monoterpene indole alkaloids (MIAs) from Madagascar periwinkle (Catharanthus roseus) are secondary metabolites of high interest due to their therapeutical values. Secologanin, the monoterpenoid moiety incorporated into MIAs, is derived from the plastidial methyl-D: -erythritol 4-phosphate (MEP) pathway. Here, we h...

journal_title：Plant molecular biology

authors： Oudin A,Mahroug S,Courdavault V,Hervouet N,Zelwer C,Rodríguez-Concepción M,St-Pierre B,Burlat V

更新日期：2007-09-01 00:00:00

abstract：:Unicellular algae grow well under limiting CO2 conditions, aided by a carbon concentrating mechanism (CCM). In C. reinhardtii, this mechanism is inducible and is present only in cells grown under low CO2 conditions. We constructed a cDNA library from cells adapting to low CO2, and screened the library for cDNAs specif...

journal_title：Plant molecular biology

authors： Burow MD,Chen ZY,Mouton TM,Moroney JV

更新日期：1996-05-01 00:00:00

abstract：:In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...

journal_title：Plant molecular biology

authors： Foley RC,Liang ZM,Singh KB

更新日期：1997-03-01 00:00:00

abstract：:The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...

journal_title：Plant molecular biology

authors： Paiva NL,Edwards R,Sun YJ,Hrazdina G,Dixon RA

更新日期：1991-10-01 00:00:00

abstract：:Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...

journal_title：Plant molecular biology

authors： Lawton K,Ward E,Payne G,Moyer M,Ryals J

更新日期：1992-08-01 00:00:00

abstract：:With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional ...

journal_title：Plant molecular biology

authors： Hirochika H,Guiderdoni E,An G,Hsing YI,Eun MY,Han CD,Upadhyaya N,Ramachandran S,Zhang Q,Pereira A,Sundaresan V,Leung H

更新日期：2004-02-01 00:00:00

abstract：:An 8 bp sequence repeated 6 times is present to the right of the mannopine type pRi8196 T-DNA right-border sequence. Experiments were designed to test whether these repeats have a role in T-DNA transfer. Several constructs in which different lengths of pRi8196 right-border region were linked to the cucumopine synthesi...

journal_title：Plant molecular biology

authors： Hansen G,Tempé J,Brevet J

更新日期：1992-10-01 00:00:00

abstract：:The immunoscreening method was used to isolate cDNAs of 1323 bp (ClOCT1) and 1433 bp (ClOCT2) encoding two ornithine carbamoyltransferases (OCT, EC 2.1.3.3) from the cDNA expression library of Canavalia lineata leaves constructed in a lambdaZAP Express vector. ClOCT1 and ClOCT2 encode 359 and 369 amino acids, respecti...

journal_title：Plant molecular biology

authors： Lee Y,Choi YA,Hwang ID,Kim SG,Kwon YM

更新日期：2001-08-01 00:00:00

abstract：:Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...

journal_title：Plant molecular biology

authors： Hellens RP,Edwards EA,Leyland NR,Bean S,Mullineaux PM

更新日期：2000-04-01 00:00:00

abstract：:When subjected to salt stress or drought, some vascular plants such as barley respond with an increased accumulation of the osmoprotectant glycine betaine (betaine), being the last step of betaine synthesis catalyzed by betaine aldehyde dehydrogenase (BADH). We report here cloning and characterization of BADH cDNA fro...

journal_title：Plant molecular biology

authors： Ishitani M,Nakamura T,Han SY,Takabe T

更新日期：1995-01-01 00:00:00

abstract：:Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on waln...

journal_title：Plant molecular biology

authors： Brasileiro AC,Leplé JC,Muzzin J,Ounnoughi D,Michel MF,Jouanin L

更新日期：1991-09-01 00:00:00

abstract：:We have isolated and characterized the genomic clone of maize casein kinase 2 (CK2) alpha subunit using the previously described alphaCK2-1 cDNA clone as a probe. The genomic clone is 7.5 kb long and contains 10 exons, separated by 9 introns of different size, two larger than 1.5 kb and the others around 100-150 bp. T...

journal_title：Plant molecular biology

authors： Peracchia G,Jensen AB,Culiáñez-Macià FA,Grosset J,Goday A,Issinger OG,Pagès M

更新日期：1999-05-01 00:00:00

abstract：:We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...

journal_title：Plant molecular biology

authors： Hildebrand M,Hasegawa P,Ord RW,Thorpe VS,Glass CA,Volcani BE

更新日期：1992-08-01 00:00:00

abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...

journal_title：Plant molecular biology

authors： Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HE

更新日期：2014-07-01 00:00:00

abstract：KEY MESSAGE:This manuscript reports the identification and characterization of five transcription factors binding to the promoter of OsNHX1 in a salt stress tolerant rice genotype (Hasawi). Although NHX1 encoding genes are known to be highly regulated at the transcription level by different abiotic stresses, namely sal...

journal_title：Plant molecular biology

authors： Almeida DM,Gregorio GB,Oliveira MM,Saibo NJ

更新日期：2017-01-01 00:00:00

abstract：:Rice provides a staple source of energy, protein and other nutrients to half of the world population. Over 90 of the rice seeds consists of starch and protein by dry weight. The quantity and property of starch and protein thus play a dominant role in the yield and quality of rice. The amylase content of starch is a de...

journal_title：Plant molecular biology

authors： Duan M,Sun SS

更新日期：2005-09-01 00:00:00

abstract：:Cosmid binary vectors for the introduction of DNA into plant cells have been constructed. These vectors are derived from the replicon of the broad host range plasmid pRK2 and contain the T-DNA border regions between which have been placed a chimaeric gene conferring resistance to kanamycin in plant cells. Appropriate ...

journal_title：Plant molecular biology

authors： van den Elzen P,Lee KY,Townsend J,Bedbrook J

更新日期：1985-05-01 00:00:00

abstract：:Herbaspirillum seropedicae is a diazotrophic and endophytic bacterium that associates with economically important grasses promoting plant growth and increasing productivity. To identify genes related to bacterial ability to colonize plants, wheat seedlings growing hydroponically in Hoagland's medium were inoculated wi...

journal_title：Plant molecular biology

authors： Pankievicz VC,Camilios-Neto D,Bonato P,Balsanelli E,Tadra-Sfeir MZ,Faoro H,Chubatsu LS,Donatti L,Wajnberg G,Passetti F,Monteiro RA,Pedrosa FO,Souza EM

更新日期：2016-04-01 00:00:00

abstract：:There was no discernible effect after incubating recombinant Anabaena Rubisco and carboxyarabinitol 1-phosphate with the product of the Anabaena rca gene. Since the unactivated cyanobacterial Rubisco is not readily inhibited by ribulose 1,5-bisphosphate and fallover is not observed, a genetic basis for the function of...

journal_title：Plant molecular biology

authors： Li LA,Zianni MR,Tabita FR

更新日期：1999-06-01 00:00:00

abstract：:Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...

journal_title：Plant molecular biology

authors： Altschuler M,Mascarenhas JP

更新日期：1985-09-01 00:00:00

abstract：:Using RNA from developing cotyledons and leaves of bean two products were synthesizedin vitro, with Mr 31000 and 31500 which are antigenically related to the subunit of phytoferritin (Mr 26500). The relative abundance of the two products is dependent on the type of tissue from which the RNA was derived. Evidence is pr...

journal_title：Plant molecular biology

authors： van der Mark F,Klerk H,Bienfait F

更新日期：1983-11-01 00:00:00

abstract：:Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...

journal_title：Plant molecular biology

authors： Pontier D,Gan S,Amasino RM,Roby D,Lam E

更新日期：1999-04-01 00:00:00

abstract：:The treatment of barley aleurone layers with gibberellic acid (GA3) results in the synthesis of two groups of α-amylase isozymes. Addition of abscisic acid (ABA) at the same time as GA3 inhibited the synthesis of both groups of isozymes. However, midcourse ABA addition (12 h or later after GA3) had a more inhibitory e...

journal_title：Plant molecular biology

authors： Nolan RC,Lin LS,Ho TH

更新日期：1987-01-01 00:00:00

abstract：KEY MESSAGE:Overexpression of BoMYB29 gene up-regulates the aliphatic glucosinolate pathway in Brassica oleracea plants increasing the production of the anti-cancer metabolite glucoraphanin, and the toxic and pungent sinigrin. Isothiocyanates, the bio-active hydrolysis products of glucosinolates, naturally produced by ...

journal_title：Plant molecular biology

authors： Zuluaga DL,Graham NS,Klinder A,van Ommen Kloeke AEE,Marcotrigiano AR,Wagstaff C,Verkerk R,Sonnante G,Aarts MGM

更新日期：2019-09-01 00:00:00

abstract：:Golgins are a family of coiled-coil proteins that are associated with the Golgi apparatus. They are necessary for tethering events in membrane fusion and may act as structural support for Golgi cisternae. Here we report on the identification of an Arabidopsis golgin which is a homologue of CASP, a known transmembrane ...

journal_title：Plant molecular biology

authors： Renna L,Hanton SL,Stefano G,Bortolotti L,Misra V,Brandizzi F

更新日期：2005-05-01 00:00:00

abstract：:Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...

journal_title：Plant molecular biology

authors： van der Salm T,Bosch D,Honée G,Feng L,Munsterman E,Bakker P,Stiekema WJ,Visser B

更新日期：1994-10-01 00:00:00

abstract：:Wood is the most abundant biological resource on earth and it is also an important raw material for a major global industry with rapidly increasing demand. The genus Eucalyptus includes the most widely used tree species for industrial plantation, mainly for making pulp and paper. With the aim of identifying major gene...

journal_title：Plant molecular biology

authors： Paux E,Tamasloukht M,Ladouce N,Sivadon P,Grima-Pettenati J

更新日期：2004-05-01 00:00:00