Abstract:
:There was no discernible effect after incubating recombinant Anabaena Rubisco and carboxyarabinitol 1-phosphate with the product of the Anabaena rca gene. Since the unactivated cyanobacterial Rubisco is not readily inhibited by ribulose 1,5-bisphosphate and fallover is not observed, a genetic basis for the function of the Rubisco activase-like gene (rca) was sought. The monocistronic rca gene was inactivated in vivo and resulting mutant strains of A. variabilis were found to be incapable of synthesizing immunologically detected RCA protein. The requirement for the product of the rca gene in the light was further examined by measuring Rubisco activity in permeabilized whole cells of wild-type and rca mutant strains at different light intensities. In a 1% CO2-air atmosphere, inactivation of rca reduced the ability of A. variabilis to elevate Rubisco activity under high light (73 micromol quanta m(-2) s(-1)), but had little effect under low light (8 micromol m(-2) s(-1)). For air-grown cultures, differences in the rates exhibited by the wild-type and rca mutant to fully activate Rubisco during a whole-cell assay were enhanced by increases in light intensity. The significance of the rca mutation was underlined by effects on growth as, unlike the wild-type, growth rates did not increase after cells transferred from low to high light intensities. Higher exogenous CO2 concentrations (1%) were required to sustain a normal growth rate for the A. variabilis rca mutant. When grown in air levels of CO2, the rca mutant not only needed longer times to double in cell density but also exhibited greatly diminished Rubisco activity compared with the wild-type strain. Despite the unusual properties of cyanobacterial Rubisco, these results suggest a physiological role for the product of the rca gene in maximizing the activity of Rubisco in heterocystous cyanobacteria.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Li LA,Zianni MR,Tabita FRdoi
10.1023/a:1006251808625subject
Has Abstractpub_date
1999-06-01 00:00:00pages
467-78issue
3eissn
0167-4412issn
1573-5028journal_volume
40pub_type
杂志文章abstract::The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9138-y
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abstract::The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...
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doi:10.1007/s11103-014-0252-3
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005728411921
更新日期:1997-02-01 00:00:00
abstract::The small GTPases of the Rho family play a key role in actin cytoskeletal organization. In plants, a novel Rho subfamily, called ROP (Rho of plants), has been found. In Arabidopsis, 12 ROP GTPases have been identified which differ mainly at their C-termini. To test the localization of two members of this subfamily (At...
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journal_title:Plant molecular biology
pub_type: 杂志文章
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journal_title:Plant molecular biology
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journal_title:Plant molecular biology
pub_type: 杂志文章,评审
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1986-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000036368.74758.66
更新日期:2004-02-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00026794
更新日期:1992-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0069-5
更新日期:2013-07-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006355510883
更新日期:2000-03-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016069
更新日期:1991-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039532
更新日期:1994-10-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027070
更新日期:1992-08-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1993-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015983
更新日期:1993-06-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2020-07-01 00:00:00
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journal_title:Plant molecular biology
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更新日期:2010-09-01 00:00:00
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journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040578
更新日期:1994-01-01 00:00:00
abstract::In animals and fungi, small cysteine-rich proteins called metallothioneins (MTs) play a role in heavy metal tolerance. MT genes have been isolated in plants, but their function remains to be elucidated. We have isolated two distinct Vicia faba MT genes that belong to the type 1 group of plant MT genes in contrast to a...
journal_title:Plant molecular biology
pub_type: 杂志文章
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