A census of carbohydrate-active enzymes in the genome of Arabidopsis thaliana.

Abstract:

:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdown (glycoside hydrolases and polysaccharide lyases). The importance of these processes is reflected in the dedication of 1-2% of an organism's genes to glycoside hydrolases and glycosyltransferases alone. In plants, these processes are of particular importance for cell-wall synthesis and expansion. starch metabolism, defence against pathogens, symbiosis and signalling. Here we present an analysis of over 730 open reading frames representing the two main classes of carbohydrate-active enzymes, glycoside hydrolases and glycosyltransferases, in the genome of Arabidopsis thaliana. The vast importance of these enzymes in cell-wall formation and degradation is revealed along with the unexpected dominance of pectin degradation in Arabidopsis, with at least 170 open-reading frames dedicated solely to this task.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Henrissat B,Coutinho PM,Davies GJ

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

55-72

issue

1-2

eissn

0167-4412

issn

1573-5028

journal_volume

47

pub_type

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