Abstract：
:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these genes. This repetitive element, named CR1, has been characterized via sequencing, genetic mapping and hybridization to related solanaceous species. Results indicate that there are as many as 30 copies of this element in the tomato genome and that most, if not all, are found at independent loci. Sites corresponding to 12 of the repeats have been located on different regions of chromosomes 2, 4, 5, 7, 10 and 11. A 1.6 kb PstI-EcoRI fragment from the Cab-1 locus containing the element was sequenced and found to be 75% AT-rich. No open reading frames larger than 150 bp were detected. Several imperfect inverted repeats flanked by direct repeats could be found at the ends of the element. This arrangement is reminiscent of known transposons. Southern hybridization analysis indicates that multiple copies of CR1 exist in all species of the genus Lycopersicon as well as in Solanum lycopersicoides and S. tuberosum (potato), but not in eggplant, pepper, petunia, Datura or tobacco. Melt-off experiments indicate that members of the CR1 family in the tomato genome are more closely related to one another than to homologous members in the genomes of S. lycopersicoides or S. tuberosum, suggesting some type of concerted evolution.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Bernatzky R,Pichersky E,Malik VS,Tanksley SDdoi
10.1007/BF00014948subject
Has Abstractpub_date
1988-09-01 00:00:00pages
423-33issue
5eissn
0167-4412issn
1573-5028journal_volume
10pub_type
杂志文章abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9585-8
更新日期：2010-03-01 00:00:00
abstract：:Synthetic oligonucleotides corresponding to conserved regions of cysteine proteinases were used as primers in the RT-PCR amplification of a fragment of cDNA corresponding to a region of a cysteine proteinase gene expressed during germination of chickpea (cac for Cicer arietinum cysteine proteinase). The identity of th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023238
更新日期：1994-05-01 00:00:00
abstract：:To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040693
更新日期：1994-11-01 00:00:00
abstract：:The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040578
更新日期：1994-01-01 00:00:00
abstract：:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0195-8
更新日期：2014-07-01 00:00:00
abstract：:Because symbionts are experiencing major morphological changes during ectomycorrhiza development, the expression of genes encoding cytoskeletal proteins is likely altered. To test this contention, we have cloned and characterized in a alpha-tubulin cDNA (EgTubA1) from Eucalyptus globulus. A poorly-aggressive isolate (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019477
更新日期：1996-07-01 00:00:00
abstract：:cDNAs for senescence-inducible genes were isolated by differential hybridization from a cDNA library derived from mRNAs from the petals of rose flowers. The amino acid sequence deduced from these cDNAs exhibited significant homology to those of delta 9 acyl-lipid desaturases of cyanobacteria and of delta 9 acyl-CoA de...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00041154
更新日期：1995-11-01 00:00:00
abstract：:Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9229-9
更新日期：2007-11-01 00:00:00
abstract：:RNA editing of T-urf13, a gene specific to the mitochondria of cytoplasmic male-sterile, type-T (cms-T) maize, and an adjacent, cotranscribed gene orf221, have been studied by cDNA sequencing. No editing was detected in 22 cDNA clones. This is the only report of a polypeptide-encoding gene in higher-plant mitochondria...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00037148
更新日期：1991-11-01 00:00:00
abstract：:The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023564
更新日期：1994-02-01 00:00:00
abstract：:Lysine residue 9 of histone H3 can either be acetylated or mono-, di-, or tri-methylated. These epigenetic states have a diverse impact on regulating gene transcriptional activity and chromatin organization. H3K9ac is invariably correlated with transcriptional activation, whereas H3K9me2 has been reported to be mainly...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9594-7
更新日期：2010-04-01 00:00:00
abstract：:Plant isoprenoids are derived from two independent pathways, the cytosolic mevalonate pathway and the plastid methylerythritol 4-phosphate (MEP) pathway. We used green fluorescent fusion protein assays to demonstrate that the Arabidopsis MEP pathway enzymes are localized to the chloroplast. We have also characterized ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9297-5
更新日期：2008-04-01 00:00:00
abstract：:Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9866-x
更新日期：2012-02-01 00:00:00
abstract：:The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0252-3
更新日期：2014-12-01 00:00:00
abstract：:Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9441-2
更新日期：2009-03-01 00:00:00
abstract：:Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0445-z
更新日期：2016-05-01 00:00:00
abstract：:Geminiviruses encode a few proteins and depend on cellular factors to complete their replicative cycle. As a way to understand geminivirus-host interactions, we have searched for cellular proteins which interact with viral proteins. By using the yeast two-hybrid technology and the wheat dwarf geminivirus (WDV) RepA pr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006138221874
更新日期：1999-03-01 00:00:00
abstract：:The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0317-y
更新日期：2015-06-01 00:00:00
abstract：:A clone encoding a proline-rich protein (ZmPRP) has been obtained from maize root by differential screening of a maturing elongation root cDNA library. The amino acid sequence deduced from the full-length cDNA contains a putative signal peptide and a highly repetitive sequence containing the PEPK motif, indicating tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006129703262
更新日期：1999-03-01 00:00:00
abstract：:Co-suppression of host genes and 35S transgenes encoding nitrate reductase was previously reported in transgenic tobacco plants (Nicotiana tabacum cv. Paraguay or Burley) using either a full-length cDNA or fragments devoid of the 3' and/or 5' UTR. Co-suppression was previously shown to affect a limited fraction of the...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019126
更新日期：1995-10-01 00:00:00
abstract：:The High Mobility Group (HMG) proteins of vertebrate animals have been the subject of intensive study because of evidence that they may be structural proteins of transcriptionally active chromatin. Organisms other than vertebrate animals have chromatin proteins which meet the operational criteria of salt extractabilit...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015875
更新日期：1987-09-01 00:00:00
abstract：KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0808-8
更新日期：2019-01-01 00:00:00
abstract：:The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006058700720
更新日期：1998-06-01 00:00:00
abstract：:Seed dormancy is an important developmental process that prevents pre-harvest sprouting in many grains and other seeds. Abscisic acid (ABA), a plant hormone, plays a crucial role in regulating dormancy but the underlying molecular regulatory mechanisms are not fully understood. An Arabidopsis zinc-finger gene, MEDIATO...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000028730.10834.e3
更新日期：2004-01-01 00:00:00
abstract：:Tandemly arrayed genes (TAGs) account for about one-third of the duplicated genes in eukaryotic genomes. They provide raw genetic material for biological evolution, and play important roles in genome evolution. The 22-kDa prolamin genes in cereal genomes represent typical TAG organization, and provide the good materia...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9705-5
更新日期：2010-12-01 00:00:00
abstract：:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027495
更新日期：1990-04-01 00:00:00
abstract：:A cDNA coding for phosphoenolpyruvate carboxylase (PEPC) was isolated from a cDNA library from Solanum tuberosum and the sequence of the cDNA was determined. It was inserted into a bacterial expression vector and a PEPC- Escherichia coli mutant could be complemented by the cDNA construct. A functional fusion protein c...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00021542
更新日期：1993-11-01 00:00:00
abstract：:A cDNA clone encoding Arabidopsis thaliana galactokinase was fortuitously isolated during the course of a screen for plant homologues of a Saccharomyces cerevisiae peroxisome assembly gene, PAS9. Clones were sought which restored the ability of pas9 cells to grow on oleate as a sole carbon source, as oleate metabolism...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005816104743
更新日期：1997-06-01 00:00:00
abstract：:Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9849-y
更新日期：2012-01-01 00:00:00
abstract：KEY MESSAGE:The crop yield losses induced by phytoviruses are mainly associated with the symptoms of the disease. DNA modifications as methylation can modulate the information coded by the sequence, process named epigenetics. Viral infection can change the expression patterns of different genes linked to defenses and s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-01051-6
更新日期：2020-11-01 00:00:00
