GRAB proteins, novel members of the NAC domain family, isolated by their interaction with a geminivirus protein.

abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...

journal_title：Plant molecular biology

authors： Panpetch P,Field RA,Limpaseni T

更新日期：2018-03-01 00:00:00

abstract：:A 47 kDa glycoprotein, termed EP4, was purified from carrot cell suspension culture medium. An antiserum raised against EP4 also recognized a protein of 45 kDa that was ionically bound to the cell wall. EP4 was detected in culture media from both embryogenic and non-embryogenic cell lines and was found to be secreted ...

journal_title：Plant molecular biology

authors： van Engelen FA,de Jong AJ,Meijer EA,Kuil CW,Meyboom JK,Dirkse WG,Booij H,Hartog MV,Vandekerckhove J,de Vries SC

更新日期：1995-03-01 00:00:00

abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...

journal_title：Plant molecular biology

authors： Boston RS,Viitanen PV,Vierling E

更新日期：1996-10-01 00:00:00

abstract：:H(+)-ATPase cDNAs were identified in a potato leaf library using an Arabidopsis gene as a probe. Based on their sequences, the clones could be grouped into at least two classes. A similar classification was obtained from the analysis of sequence data from four tobacco genes. Both potato genes are expressed in all tiss...

journal_title：Plant molecular biology

authors： Harms K,Wöhner RV,Schulz B,Frommer WB

更新日期：1994-11-01 00:00:00

abstract：:More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group va...

journal_title：Plant molecular biology

authors： Perlick AM,Pühler A

更新日期：1993-09-01 00:00:00

abstract：:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...

journal_title：Plant molecular biology

authors： Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

更新日期：1990-04-01 00:00:00

abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...

journal_title：Plant molecular biology

authors： Leisy DJ,Hnilo J,Zhao Y,Okita TW

更新日期：1990-01-01 00:00:00

abstract：:We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical ...

journal_title：Plant molecular biology

authors： Köhler U,Cerff R,Brinkmann H

更新日期：1996-01-01 00:00:00

abstract：:Brachypodium distachyon (Brachypodium) has been recently recognized as an emerging model system for both comparative and functional genomics in grass species. In this study, 55,221 repeat masked Brachypodium BAC end sequences (BES) were used for comparative analysis against the 12 rice pseudomolecules. The analysis re...

journal_title：Plant molecular biology

authors： Huo N,Vogel JP,Lazo GR,You FM,Ma Y,McMahon S,Dvorak J,Anderson OD,Luo MC,Gu YQ

更新日期：2009-05-01 00:00:00

abstract：:Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...

journal_title：Plant molecular biology

authors： Hsieh WL,Wolniak SM

更新日期：1998-05-01 00:00:00

abstract：:Members of the plant-specific GRAS family of putative transcription factors are involved in various aspects of plant development. SCARECROW (SCR) is a member of this protein family and plays a significant role in the radial patterning of both roots and shoots. However, little is known about the regulation of SCR expre...

journal_title：Plant molecular biology

authors： Gao MJ,Parkin I,Lydiate D,Hannoufa A

更新日期：2004-05-01 00:00:00

abstract：:HSP100 protein is an important component of the heat-shock response in diverse organisms. Using specific primers based on cDNA sequence, rice hsp101 gene was PCR-amplified and sequenced. Southern analysis revealed that there appears to be a single gene per haploid genome coding for HSP101 protein in rice. Northern ana...

journal_title：Plant molecular biology

authors： Agarwal M,Sahi C,Katiyar-Agarwal S,Agarwal S,Young T,Gallie DR,Sharma VM,Ganesan K,Grover A

更新日期：2003-03-01 00:00:00

abstract：:Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...

journal_title：Plant molecular biology

authors： Mahfouz MM,Li L,Piatek M,Fang X,Mansour H,Bangarusamy DK,Zhu JK

更新日期：2012-02-01 00:00:00

abstract：:Efficient chloroplast transformation systems now available allow the manipulation of the evolutionarily highly conserved psbA gene in the eucaryotic organism Chlamydomonas reinhardtii. Two copies of this gene in the inverted repeat region of the chloroplast genome contain four large group I introns. To analyse possibl...

journal_title：Plant molecular biology

authors： Johanningmeier U,Heiss S

更新日期：1993-04-01 00:00:00

abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

journal_title：Plant molecular biology

authors： Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

更新日期：2002-02-01 00:00:00

abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...

journal_title：Plant molecular biology

authors： de la Paz Sánchez M,Torres A,Boniotti MB,Gutierrez C,Vázquez-Ramo JM

更新日期：2002-09-01 00:00:00

abstract：:The large number of mitogen-activated protein (MAP) kinase genes identified to date in plants suggests that their encoded proteins have a wide array of functions in development and physiological responses, as has been indicated by studies on the factors which lead to the activation of these kinases. Signalling pathway...

journal_title：Plant molecular biology

authors： Voronin V,Touraev A,Kieft H,van Lammeren AA,Heberle-Bors E,Wilson C

更新日期：2001-04-01 00:00:00

abstract：:Plants produce ethylene in response to many biotic and abiotic stresses. In response to ozone the foliage of potato plants sequentially expressed two ACC synthase genes (ST-ACS4, ST-ACS5). The same expression pattern of the two genes also occurred in response to Cu2+ and infection with Alternaria solani. ST-ACS5 expre...

journal_title：Plant molecular biology

authors： Schlagnhaufer CD,Arteca RN,Pell EJ

更新日期：1997-12-01 00:00:00

abstract：:The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...

journal_title：Plant molecular biology

authors： Luque I,Flores E,Herrero A

更新日期：1993-03-01 00:00:00

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

journal_title：Plant molecular biology

authors： Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

更新日期：1999-02-01 00:00:00

abstract：:A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

journal_title：Plant molecular biology

authors： Atanassova R,Flénet M,Gigot C,Chaubet N

更新日期：1998-05-01 00:00:00

abstract：:A novel rice genomic sequence encoding coding segments homologous to other metallothionein-like genes was isolated from Oryza sativa genomic library. This sequence, hereby designated as rgMT (rice genomic metallothionein-like gene), consists of two exons and one intron. From the coding sequence, it is predicted that r...

journal_title：Plant molecular biology

authors： Hsieh HM,Liu WK,Huang PC

更新日期：1995-06-01 00:00:00

abstract：:The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...

journal_title：Plant molecular biology

authors： Montoliu L,Rigau J,Puigdomènech P

更新日期：1990-01-01 00:00:00

abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

journal_title：Plant molecular biology

authors： Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

更新日期：2020-08-01 00:00:00

abstract：:We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...

journal_title：Plant molecular biology

authors： Fujimoto S,Matsunaga S,Yonemura M,Uchiyama S,Azuma T,Fukui K

更新日期：2004-09-01 00:00:00

abstract：:Two subtractive cDNA libraries were developed to study genes associated with bud dormancy (reverse library) and initiation of shoot growth (forward library) in leafy spurge. To identify unique sequences represented in each library, 15744 clones were screened to reduce the level of redundancy within both libraries. A t...

journal_title：Plant molecular biology

authors： Jia Y,Anderson JV,Horvath DP,Gu YQ,Lym RG,Chao WS

更新日期：2006-05-01 00:00:00

abstract：:As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...

journal_title：Plant molecular biology

authors： Eckardt NA,McHenry L,Guiltinan MJ

更新日期：1998-11-01 00:00:00

abstract：:T-DNA tagging is a high throughput strategy for identifying and cloning functional genes in plants. In this study, we screened 4416 lab-created T(1) rice T-DNA tagged lines and identified a mutant, designated dh1 (degenerated hull1), with phenotype of degenerated hull and naked pistils and stamens. Approximately 60% f...

journal_title：Plant molecular biology

authors： Li A,Zhang Y,Wu X,Tang W,Wu R,Dai Z,Liu G,Zhang H,Wu C,Chen G,Pan X

更新日期：2008-03-01 00:00:00

abstract：:Plants cope with pathogens with distinct mechanisms. One example is a gene-for-gene system, in which plants recognize the pathogen molecule by specified protein(s), this being called the R factor. However, mechanisms of interaction between proteins from the host and the pathogen are not completely understood. Here, we...

journal_title：Plant molecular biology

authors： Ueda H,Yamaguchi Y,Sano H

更新日期：2006-05-01 00:00:00