Expression of a rice glutelin promoter in transgenic tobacco.

Abstract:

:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days after flowering and obtained a maximum level at 16 days after flowering, the onset of overall protein accumulation. Significant expression of CAT activity in non-seed tissues occurred in some, but not all plants, suggesting possible chromosome position effects on non-seed tissue expression. A positive correlation was observed between expression levels in seeds and gene copy numbers.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Leisy DJ,Hnilo J,Zhao Y,Okita TW

doi

10.1007/BF00015653

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

41-50

issue

1

eissn

0167-4412

issn

1573-5028

journal_volume

14

pub_type

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