Abstract：
:A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion mutants and examined the transient expression of beta-glucuronidase reporter gene by particle bombardment and the stable expression by Agrobacterium-mediated transformation of tobacco plants. Positive regulatory elements were located in the -894/-602 and -602/-74 regions of the Con A gene, and in the -428/-376, -281/-155 and -155/-50 regions of the canavalin gene. In addition, the results suggested that the A/T-rich sequences in the 5'-upstream region of the Con A gene play a role in transcriptional activation, but that those of the canavalin gene have little effect on the gene expression. The CATGCAT/A sequence was not sufficient by itself for high levels of expression of both the Con A and canavalin genes. The canavalin polypeptide amounted to about 1% of the total extractable protein in the transgenic tobacco seeds, but the Con A polypeptide was not detected in the extractable protein.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Yamamoto S,Nishihara M,Morikawa H,Yamauchi D,Minamikawa Tdoi
10.1007/BF00020226subject
Has Abstractpub_date
1995-02-01 00:00:00pages
729-41issue
4eissn
0167-4412issn
1573-5028journal_volume
27pub_type
杂志文章abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005889314967
更新日期：1997-05-01 00:00:00
abstract：:The expression of a 25 kDa protein, encoded by the fused mitochondrial pcf gene, is associated with cytoplasmic male sterility (CMS) in petunia. To investigate the role of the 25 kDa protein in CMS we have transformed petunia and tobacco plants with constructs expressing a portion of the urfS sequence of the pcf cDNA ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042040
更新日期：1995-04-01 00:00:00
abstract：KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-018-0771-4
更新日期：2018-09-01 00:00:00
abstract：:A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016515
更新日期：1990-05-01 00:00:00
abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1013644026132
更新日期：2001-12-01 00:00:00
abstract：:During flower development, pluripotent stem cells within the floral meristem give rise to proliferative precursor cells whose progeny eventually acquire specialized functions within each floral organ. The regulatory mechanisms by which plant cells transition from a proliferating state to a differentiated state are not...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9844-3
更新日期：2012-02-01 00:00:00
abstract：:The PsbS gene product (PSII-S) which is an integral subunit of photosystem II has recently been reported to be a new type of pigment-binding protein [11]. The chlorophylls of the PSII-S protein exhibit weak excitonic coupling and this protein is stable also in the absence of pigments. Here we investigated the expressi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019467
更新日期：1996-07-01 00:00:00
abstract：:Wood is the most abundant biological resource on earth and it is also an important raw material for a major global industry with rapidly increasing demand. The genus Eucalyptus includes the most widely used tree species for industrial plantation, mainly for making pulp and paper. With the aim of identifying major gene...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-0621-4
更新日期：2004-05-01 00:00:00
abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019182
更新日期：1995-01-01 00:00:00
abstract：:The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019103
更新日期：1996-11-01 00:00:00
abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006148815106
更新日期：1999-02-01 00:00:00
abstract：:Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029145
更新日期：1992-10-01 00:00:00
abstract：:SAUR-AC1 is a small-auxin-up-RNA (SAUR) gene of Arabidopsis. Here we demonstrate that the SAUR-AC1 promoter region induces accumulation of a reporter transcript upon treatment with auxin and is preferentially active in elongating hypocotyls and certain other organs and tissues of transgenic Arabidopsis and tobacco pla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005875300606
更新日期：1997-07-01 00:00:00
abstract：:The barley BARE-1 is a transcribed, copia-like retroelement with well-conserved functional domains, an active promoter, and a copy number of at least 3 x 10(4). We examined its chromosomal localization by in situ hybridization. The long terminal repeat (LTR) probe displayed a uniform hybridization pattern over the who...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019563
更新日期：1996-03-01 00:00:00
abstract：:We have analysed the expression of the 8-10 members of the gene family encoding the flavonoid biosynthetic enzyme chalcone synthase (CHS) from Petunia hybrida. During normal plant development only two members of the gene family (CHS-A and CHS-J) are expressed. Their expression is restricted to floral tissues mainly. A...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020506
更新日期：1989-02-01 00:00:00
abstract：:Screening of a lambda gt11 cDNA expression library of Euglena gracilis with antibodies directed against histones H2 from maize resulted in the isolation of a full-length cDNA for a histone H2A. The open-reading frame of 408 bp corresponded to a protein of 136 amino acid residues (14 kDa). Despite the presence of a pol...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014447
更新日期：1994-03-01 00:00:00
abstract：:Chimeric genes composed of the beta-phaseolin promoter, an alpha-zein coding sequence and its modified versions containing lysine codons, and a beta-zein polyadenylation signal were inserted into the genome of tobacco by Agrobacterium-mediated transformation. alpha-Zein mRNA levels in the transgenic tobacco seeds 20 d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017922
更新日期：1991-01-01 00:00:00
abstract：:Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019323
更新日期：1995-02-01 00:00:00
abstract：:A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich protein...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00023430
更新日期：1991-04-01 00:00:00
abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1026494813936
更新日期：1999-05-01 00:00:00
abstract：:A combined chemical, biochemical and molecular study was conducted to understand the differential accumulation of volatile sesquiterpenes in melon fruits. Sesquiterpenes were present mainly in the rinds of climacteric varieties, and a great diversity in their composition was found among varieties. Sesquiterpenes were ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9296-6
更新日期：2008-04-01 00:00:00
abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0555-7
更新日期：2017-02-01 00:00:00
abstract：:The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010665910095
更新日期：2001-03-01 00:00:00
abstract：:Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006178710443
更新日期：1999-01-01 00:00:00
abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006282507095
更新日期：1999-07-01 00:00:00
abstract：:In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019952
更新日期：1993-01-01 00:00:00
abstract：:The phytohormone abscisic acid is probably present in all higher plants. This hormone is necessary for regulation of several events during seed development and for the response to environmental stresses such as desiccation, salt and cold. An important part of the physiological response to abscisic acid is achieved thr...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1023/a:1006058700720
更新日期：1998-06-01 00:00:00
abstract：:Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040527
更新日期：1992-09-01 00:00:00
abstract：:Glyoxalase II is part of the glutathione-dependent glyoxalase detoxification system. In addition to its role in the detoxification of cytotoxic 2-oxo-aldehydes, specifically methylglyoxal, it has been suggested that the glyoxalase system may also play a role in controlling cell differentiation and proliferation. Durin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005891123344
更新日期：1997-11-01 00:00:00
abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00039383
更新日期：1996-10-01 00:00:00
