Potato granule-bound starch synthase promoter-controlled GUS expression: regulation of expression after transient and stable transformation.

abstract：:A number of A/T-rich sequences and a CATGCAT/A sequence are contained in the 5'-upstream regions of the genes encoding concanavalin A (Con A) and canavalin, two major seed storage proteins of Canavalia gladiata D.C. To study the role of these sequences in the seed-specific gene expression, we constructed 5'-deletion m...

journal_title：Plant molecular biology

authors： Yamamoto S,Nishihara M,Morikawa H,Yamauchi D,Minamikawa T

更新日期：1995-02-01 00:00:00

abstract：:Plant R2R3-MYB transcription factors are encoded by more than 100 copies of genes. In this study, we attempted to isolate some members of the R2R3-MYB superfamily involved in regulation of nitrogen fixation in legumes. A library of 300 recombinant plasmid clones containing the R2R3-MYB fragments of the superfamily was...

journal_title：Plant molecular biology

authors： Miyake K,Ito T,Senda M,Ishikawa R,Harada T,Niizeki M,Akada S

更新日期：2003-09-01 00:00:00

abstract：:Wood is the most abundant biological resource on earth and it is also an important raw material for a major global industry with rapidly increasing demand. The genus Eucalyptus includes the most widely used tree species for industrial plantation, mainly for making pulp and paper. With the aim of identifying major gene...

journal_title：Plant molecular biology

authors： Paux E,Tamasloukht M,Ladouce N,Sivadon P,Grima-Pettenati J

更新日期：2004-05-01 00:00:00

abstract：:Using a fruit-specific cDNA as a probe we isolated and sequenced the two corresponding homologous genes (Sn-1 and Sn-2) of the bell pepper (Capsicum annuum) genome. Both genes have a single intron and numerous unusual long inverted repeat sequences. The introns share 87% homology and Sn-2 contains one 450 bp additiona...

journal_title：Plant molecular biology

authors： Pozueta-Romero J,Klein M,Houlné G,Schantz ML,Meyer B,Schantz R

更新日期：1995-09-01 00:00:00

abstract：:Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...

journal_title：Plant molecular biology

authors： Sunter G,Gardiner WE,Rushing AE,Rogers SG,Bisaro DM

更新日期：1987-11-01 00:00:00

abstract：KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

journal_title：Plant molecular biology

authors： Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

更新日期：2020-08-01 00:00:00

abstract：:Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...

journal_title：Plant molecular biology

authors： Li X,Yang Y,Yao J,Chen G,Li X,Zhang Q,Wu C

更新日期：2009-04-01 00:00:00

abstract：:Telomeres, the complex nucleoprotein structures at the ends of linear eukaryotic chromosomes, along with telomerase, the enzyme that synthesizes telomeric DNA, are required to maintain a stable genome. Together, the enzyme and substrate perform this essential service by protecting chromosomes from exonucleolytic degra...

journal_title：Plant molecular biology

authors： McKnight TD,Riha K,Shippen DE

更新日期：2002-03-01 00:00:00

abstract：:The development of an efficient transformation system is a prerequisite for the molecular analysis of gene expression in plants. In crop plants, this development has been hindered by difficulties encountered both in whole plant regeneration from protoplasts and in the general insusceptibility of monocots to Agrobacter...

journal_title：Plant molecular biology

authors： Wang YC,Klein TM,Fromm M,Cao J,Sanford JC,Wu R

更新日期：1988-07-01 00:00:00

abstract：:In bacterial and animal coenzyme A (CoA) biosynthesis, pantothenate kinase (PANK) activity is critical in regulating intracellular CoA levels. Less is known about the role of PANK in plants, although a single plant isozyme from Arabidopsis, AtPANK1, was previously cloned and analyzed in vitro. We report here the chara...

journal_title：Plant molecular biology

authors： Tilton GB,Wedemeyer WJ,Browse J,Ohlrogge J

更新日期：2006-07-01 00:00:00

abstract：:Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...

journal_title：Plant molecular biology

authors： Tsabary G,Shani Z,Roiz L,Levy I,Riov J,Shoseyov O

更新日期：2003-01-01 00:00:00

abstract：:Mammalian E2F transcription factors are composed of E2F and DP subunits, and with their negative regulators, Rb-related proteins, govern expression of cell-division-related genes. Six E2Fs and two DPs are present in mammals, but only single E2F genes are known from wheat, tobacco and carrot. Here we show that E2Fs are...

journal_title：Plant molecular biology

authors： de Jager SM,Menges M,Bauer UM,Murra JA

更新日期：2001-11-01 00:00:00

abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...

journal_title：Plant molecular biology

authors： Bernatzky R,Pichersky E,Malik VS,Tanksley SD

更新日期：1988-09-01 00:00:00

abstract：KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...

journal_title：Plant molecular biology

authors： Liu JJ,Williams H,Li XR,Schoettle AW,Sniezko RA,Murray M,Zamany A,Roke G,Chen H

更新日期：2017-11-01 00:00:00

abstract：:During maize seed development, endosperm cells synthesize large amounts of storage proteins, alpha-, beta-, and gamma-zeins, which accumulate within endoplasmic reticulum (ER)-derived protein bodies. The absence of lysine in all zein polypeptides results in an imbalance in the amino acid composition of maize seeds. We...

journal_title：Plant molecular biology

authors： Torrent M,Alvarez I,Geli MI,Dalcol I,Ludevid D

更新日期：1997-05-01 00:00:00

abstract：:Nodules of tropical legumes generally export symbiotically fixed nitrogen in the form of ureides that are produced by oxidation of de novo synthesized purines. To investigate the regulation of de novo purine biosynthesis in these nodules, we have isolated cDNA clones encoding 5-aminoimidazole ribonucleotide (AIR) carb...

journal_title：Plant molecular biology

authors： Chapman KA,Delauney AJ,Kim JH,Verma DP

更新日期：1994-01-01 00:00:00

abstract：:Rice has developed several morphological and physiological strategies to adapt to phosphate starvation in the soil. In order to elucidate the molecular basis of response to phosphate starvation, we performed mRNA sequencing of 4 rice cultivars with variation in growth response to Pi starvation as indicated by the shoo...

journal_title：Plant molecular biology

authors： Oono Y,Kawahara Y,Yazawa T,Kanamori H,Kuramata M,Yamagata H,Hosokawa S,Minami H,Ishikawa S,Wu J,Antonio B,Handa H,Itoh T,Matsumoto T

更新日期：2013-12-01 00:00:00

abstract：:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...

journal_title：Plant molecular biology

authors： Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

更新日期：1992-02-01 00:00:00

abstract：:We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...

journal_title：Plant molecular biology

authors： Vorst O,van Dam F,Oosterhoff-Teertstra R,Smeekens S,Weisbeek P

更新日期：1990-04-01 00:00:00

abstract：:The complex capitulum of Chrysanthemum morifolium is often comprised of bilaterally symmetrical ray florets and radially symmetrical disc florets. The TCP transcription factor clade CYCLOIDEA2 (CYC2) appears to play a vital role in determining floral symmetry and in regulating floral organ development in Asteraceae. O...

journal_title：Plant molecular biology

authors： Yuan C,Huang D,Yang Y,Sun M,Cheng T,Wang J,Pan H,Zhang Q

更新日期：2020-05-01 00:00:00

abstract：:Homospermidine synthase is the first specific enzyme in the biosynthesis of pyrrolizidine alkaloids. Whereas the substrates putrescine and spermidine are part of the highly dynamic polyamine pool of plants, the product homospermidine is incorporated exclusively into the necine base moiety of pyrrolizidine alkaloids. R...

journal_title：Plant molecular biology

authors： Abdelhady MI,Beuerle T,Ober D

更新日期：2009-09-01 00:00:00

abstract：:One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations. The library contains 2.3 x 10(5) recombinants and two different calmodulin cDNAs were cloned using a heterologous probe from petunia. ...

journal_title：Plant molecular biology

authors： Breton C,Chaboud A,Matthys-Rochon E,Bates EE,Cock JM,Fromm H,Dumas C

更新日期：1995-01-01 00:00:00

abstract：:In this paper we present the structural analysis of the 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family from Petunia hybrida. Southern blot analysis and restriction endonuclease mapping showed that two cloned regions of the petunia genome contained sequences highly homologous to a previously isolated ACC o...

journal_title：Plant molecular biology

authors： Tang X,Wang H,Brandt AS,Woodson WR

更新日期：1993-12-01 00:00:00

abstract：:The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA proc...

journal_title：Plant molecular biology

authors： Lermontova I,Schubert V,Börnke F,Macas J,Schubert I

更新日期：2007-11-01 00:00:00

abstract：:A near full-length cDNA clone (pZRP2) was isolated from a cDNA library constructed from maize root mRNAs. The predicted polypeptide has a calculated molecular mass of 66,975 Da, is largely hydrophilic, and contains 26 repeats of a motif the consensus sequence of which is RKATTSYG[S][D/E][D/E][D/E][D/E][P]. The functio...

journal_title：Plant molecular biology

authors： Held BM,John I,Wang H,Moragoda L,Tirimanne TS,Wurtele ES,Colbert JT

更新日期：1997-10-01 00:00:00

abstract：:Transcription of plastid genes in higher plants is driven by two RNA polymerases. One is encoded in the chloroplast, the other is encoded in the nucleus. RNA synthesis in ribosome-deficient plastids is performed exclusively by the nuclear-encoded enzyme. In vitro capping was used to identify the transcriptional start ...

journal_title：Plant molecular biology

authors： Hübschmann T,Börner T

更新日期：1998-02-01 00:00:00

abstract：:Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...

journal_title：Plant molecular biology

authors： Tang W,Charles TM,Newton RJ

更新日期：2005-11-01 00:00:00

abstract：KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

journal_title：Plant molecular biology

authors： Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

更新日期：2020-09-01 00:00:00

abstract：:Starch-branching enzymes (SBE) alter starch structure by breaking an alpha-1,4 linkage and attaching the reducing end of the new chain to a glucan chain by an alpha 1,6 bond. In maize, three isoforms of SBE have been identified. In order to examine the function of the SBEI isoform, a reverse-genetics PCR-based screen ...

journal_title：Plant molecular biology

authors： Blauth SL,Kim KN,Klucinec J,Shannon JC,Thompson D,Guiltinan M

更新日期：2002-02-01 00:00:00

abstract：:Damage to DNA induced by ultraviolet light can be reversed by a blue light-dependent reaction catalyzed by enzymes called DNA photolyases. Chlamydomonas has been shown to have DNA photolyase activity in both the nucleus and the chloroplast. Here we report the cloning and sequencing of a gene, PHR2, from Chlamydomonas ...

journal_title：Plant molecular biology

authors： Petersen JL,Lang DW,Small GD

更新日期：1999-08-01 00:00:00