Abstract:
:The conserved protein CBF5, initially regarded as a centromere binding protein in yeast and higher plants, was later found within nucleoli and in Cajal bodies of yeast and metazoa. There, it is assumed to be involved in posttranscriptional pseudouridinylation of various RNA species that might be important for RNA processing. We found EYFP-labeled CBF5 of A. thaliana to be located within nucleoli and Cajal bodies, but neither at centromeres nor somewhere else on chromosomes. Arabidopsis mutants carrying a homozygous T-DNA insertion at the CBF5 locus were lethal. Yeast two-hybrid and mRNA expression analyses demonstrated that AtCBF5 is co-expressed and interacts with a previously uncharacterized protein containing a conserved NAF1 domain, presumably involved in H/ACA box snoRNP biogenesis. The homologous yeast protein has been shown to contribute to RNA pseudouridinylation. Thus, AtCBF5 might have an essential function in RNA processing rather than being a kinetochore protein.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Lermontova I,Schubert V,Börnke F,Macas J,Schubert Idoi
10.1007/s11103-007-9226-zsubject
Has Abstractpub_date
2007-11-01 00:00:00pages
615-26issue
5eissn
0167-4412issn
1573-5028journal_volume
65pub_type
杂志文章abstract::In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016029001537
更新日期:2002-09-01 00:00:00
abstract::The characterization of the operon encoding the alpha and beta subunits of rhodophytan (R)-phycoerythrin (PE) from the macrophytic red alga Polysiphonia boldii is reported. This plastid-encoded operon was cloned, its nucleotide sequence determined, and its expression characterized by northern and primer extension anal...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039617
更新日期:1993-01-01 00:00:00
abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015227
更新日期:1986-07-01 00:00:00
abstract::A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9768-y
更新日期:2011-05-01 00:00:00
abstract::Soybean is an important crop not only for human consumption but also for its addition of nitrogen to the soil during crop rotation. China has the largest collection of cultivated soybeans (Glycine max) and wild soybeans (Glycine soja) all over the world. The platform of soybean core, mini core and integrated applied c...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0076-6
更新日期:2013-09-01 00:00:00
abstract::The isolation of Brassica napus leaf protoplasts induces reactive oxygen species generation and accumulation in the chloroplasts. An activated isoform of NADPH oxidase-like protein was detected in the protoplasts and the protoplast chloroplasts. The purpose of this study is to define the NADH oxidase-like activities i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0252-3
更新日期:2014-12-01 00:00:00
abstract::A gene for chlorophyll a/b-binding protein (cab) of pine (Pinus thumbergii) was isolated and sequenced. The gene (cab-6) contains an intron at a position equivalent to the type II cab genes of angiosperms. Transcript mapping analyses show that the amount of the mRNA in the dark is about half of that in the light. The ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023388
更新日期:1992-06-01 00:00:00
abstract::RNA silencing is a collective term that encompasses the sequence of events that leads to the targeted degradation of cellular mRNA and thus to the silencing of corresponding gene expression. RNA silencing is initiated after introduction into the host genome of a gene that is homologous to an endogenous gene. Transcrip...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/B:PLAN.0000028797.63168.a7
更新日期:2004-01-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9322-8
更新日期:2008-07-01 00:00:00
abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0040-5
更新日期:2013-05-01 00:00:00
abstract::We investigated changes in gene expression in Iris hollandica flowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and D...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000023670.61059.1d
更新日期:2003-12-01 00:00:00
abstract::Rice seedlings accumulate stainable amounts of the 104 and 90 kDa polypeptides in response to high temperature stress. We have purified and raised highly specific polyclonal antisera against both of these polypeptides. In western blotting experiments, we find that these proteins are accumulated to different extents in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043653
更新日期:1995-10-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1021162321527
更新日期:2003-01-01 00:00:00
abstract:KEY MESSAGE:Two OsDGAT1 genes showed the ability to restore TAG and LB synthesis in yeast H1246. Alterations in the N-terminal region of OsDGAT1-1 gene revealed its regulatory role in gene function. Accumulation of triacylglycerol (TAG) or oil in vegetative tissues has emerged as a promising approach to meet the global...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01085-w
更新日期:2020-10-21 00:00:00
abstract::The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017921
更新日期:1991-01-01 00:00:00
abstract::The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006419232075
更新日期:2000-05-01 00:00:00
abstract::The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0459-6
更新日期:2016-06-01 00:00:00
abstract::Cyclins are involved in the regulation of cell cycle progression in eukaryotes. We have isolated a cyclin cDNA clone, cycZm2w, from maize root tip cells, which fits best into group A2 of current plant cyclin gene classification schemes. The cDNA encodes a protein with a domain homologous to the cyclin box of mitotic c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005968901298
更新日期:1998-05-01 00:00:00
abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9448-8
更新日期:2009-04-01 00:00:00
abstract::The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021266320877
更新日期:2002-12-01 00:00:00
abstract::Left- and right-border repeats, which surround the T-region, contain two conserved regions separated by 5 bp that are not conserved. At the onset of T-DNA processing virD-encoded proteins introduce a nick in the largest of these conserved regions (12 bp) at a specific position in the bottom strand between a guanine an...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027312
更新日期:1989-11-01 00:00:00
abstract::Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0075-7
更新日期:2013-07-01 00:00:00
abstract::Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014948
更新日期:1988-09-01 00:00:00
abstract::The major phytoalexin in alfalfa is the isoflavonoid (-)-medicarpin (or 6aR, 11aR)-medicarpin. Isoflavone reductase (IFR), the penultimate enzyme in medicarpin biosynthesis, is responsible for introducing one of two chiral centers in (-)-medicarpin. We have isolated a 1.18 kb alfalfa cDNA (pIFRalf1) which, when expres...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037051
更新日期:1991-10-01 00:00:00
abstract::Tobacco and tomato plants were generated exhibiting insect resistance due to the introduction of modified cryIA(b) and cryIC genes of Bacillus thuringiensis. Limited modifications at selected regions of the coding sequences of both genes are sufficient to obtain resistance against Spodoptera exigua, Heliothis virescen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00039519
更新日期:1994-10-01 00:00:00
abstract:KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-019-00854-6
更新日期:2019-05-01 00:00:00
abstract::A cDNA library was made from poly(A)+ RNA isolated from developing Douglas fir (Pseudotsuga menziesii) embryo and megagametophytic tissue, and the cDNA clones were identified by immunoscreening with polyclonal antiserum against the crystalloid storage protein complex of Douglas fir. The nucleotide sequence of the long...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014555
更新日期:1993-02-01 00:00:00
abstract::Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0195-8
更新日期:2014-07-01 00:00:00
abstract:KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0595-7
更新日期:2017-05-01 00:00:00
abstract::Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029004
更新日期:1993-10-01 00:00:00