Cloning and characterization of a class II DNA photolyase from Chlamydomonas.

abstract：:A cDNA clone, CGS1, encoding cystathionine gamma-synthase (CGS) from Arabidopsis thaliana was selected by complementation of CGS mutant strain of Escherichia coli (metB). Cells expressing CGS1 can grow on medium lacking Met and contain CGS enzyme activity. Genomic DNA blot analysis of A. thaliana revealed that there i...

journal_title：Plant molecular biology

authors： Kim J,Leustek T

更新日期：1996-12-01 00:00:00

abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...

journal_title：Plant molecular biology

authors： Torelli A,Soragni E,Bolchi A,Petrucco S,Ottonello S,Branca C

更新日期：1996-12-01 00:00:00

abstract：:The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 T...

journal_title：Plant molecular biology

authors： Geffers R,Cerff R,Hehl R

更新日期：2000-05-01 00:00:00

abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...

journal_title：Plant molecular biology

authors： Holmes-Davis R,Li G,Jamieson AC,Rebar EJ,Liu Q,Kong Y,Case CC,Gregory PD

更新日期：2005-02-01 00:00:00

abstract：KEY MESSAGE:This research is the first to demonstrate that OsSAUR45 is involved in plant growth though affecting auxin synthesis and transport by repressing OsYUCCA and OsPIN gene expression in rice. Small auxin-up RNAs (SAURs) comprise a large multigene family and are rapidly activated as part of the primary auxin res...

journal_title：Plant molecular biology

authors： Xu YX,Xiao MZ,Liu Y,Fu JL,He Y,Jiang DA

更新日期：2017-05-01 00:00:00

abstract：:Sucrose synthase, which cleaves sucrose in the presence of uridine diphosphate (UDP) into UDP-glucose and fructose, is thought to be a key determinant of sink strength of heterotrophic plant organs. To determine the roles of the enzyme in carrot, we characterized carrot sucrose synthase at the molecular level. Two gen...

journal_title：Plant molecular biology

authors： Sturm A,Lienhard S,Schatt S,Hardegger M

更新日期：1999-01-01 00:00:00

abstract：:Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to...

journal_title：Plant molecular biology

authors： Nakazawa Y,Hiraguri A,Moriyama H,Fukuhara T

更新日期：2007-04-01 00:00:00

abstract：:Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...

journal_title：Plant molecular biology

authors： Bennetzen JL

更新日期：2000-01-01 00:00:00

abstract：:In higher eukaryotes, the proliferating cell nuclear antigen (PCNA) can be found associated to Cyclin D and Cdk4/6, the kinase complex responsible for cell cycle commitment in response to growth and mitogenic signals. During maize germination, PCNA can be found in protein complexes between 131 and 163 kDa. The sizes o...

journal_title：Plant molecular biology

authors： de la Paz Sánchez M,Torres A,Boniotti MB,Gutierrez C,Vázquez-Ramo JM

更新日期：2002-09-01 00:00:00

abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...

journal_title：Plant molecular biology

authors： Chye ML

更新日期：1998-11-01 00:00:00

abstract：:The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...

journal_title：Plant molecular biology

authors： Dormann-Przybyl D,Strittmatter G,Kössel H

更新日期：1986-11-01 00:00:00

abstract：:The characterization of a non-photosynthetic isoform of NADP-malic enzyme (NADP-ME) from maize roots, which represents nearly 7% of the total soluble protein of this tissue, was performed. The molecular properties of the purified protein, as well as the kinetic parameters determined, indicate that the NADP-ME isoform ...

journal_title：Plant molecular biology

authors： Maurino VG,Saigo M,Andreo CS,Drincovich MF

更新日期：2001-03-01 00:00:00

abstract：:To gain insight into the regulatory mechanisms and the signals responsible for the adaptation of higher plants to conditions of varying sulfate availability, we have isolated from a sulfate-deprived root library maize cDNAs encoding sulfate permease (ZmST1) and ATP sulfurylase (ZmAS1), the two earliest components of t...

journal_title：Plant molecular biology

authors： Bolchi A,Petrucco S,Tenca PL,Foroni C,Ottonello S

更新日期：1999-02-01 00:00:00

abstract：KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...

journal_title：Plant molecular biology

authors： Okada M,Yoshida K,Takumi S

更新日期：2017-12-01 00:00:00

abstract：:A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...

journal_title：Plant molecular biology

authors： Karim S,Alezzawi M,Garcia-Petit C,Solymosi K,Khan NZ,Lindquist E,Dahl P,Hohmann S,Aronsson H

更新日期：2014-04-01 00:00:00

abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...

journal_title：Plant molecular biology

authors： Kozarewa I,Ibáñez C,Johansson M,Ogren E,Mozley D,Nylander E,Chono M,Moritz T,Eriksson ME

更新日期：2010-05-01 00:00:00

abstract：:Most plant cells retain the capacity to differentiate into all the other cell and organ types that constitute a plant. However, genome-wide transcriptional activities underlying the process of cell differentiation are poorly understood, especially in monocot plants. Here we used a rice (Oryza sativa) cell culture syst...

journal_title：Plant molecular biology

authors： Su N,He K,Jiao Y,Chen C,Zhou J,Li L,Bai S,Li X,Deng XW

更新日期：2007-02-01 00:00:00

abstract：:Plant microRNAs (miRNAs) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation. A number of miRNAs play important roles in development. In order to investigate the potential function of miRNAs during male gametogenesis in rice, we obtained both gene and small RNA express...

journal_title：Plant molecular biology

authors： Peng H,Chun J,Ai TB,Tong YA,Zhang R,Zhao MM,Chen F,Wang SH

更新日期：2012-09-01 00:00:00

abstract：:Plants, algae, cyanobacteria and many other bacteria synthesize the tetrapyrrole precursor, delta-aminolevulinic acid (ALA), from glutamate by means of a tRNAGlu-mediated pathway. The enzyme glutamyl-tRNA reductase (GTR) catalyzes the first committed step in this pathway, which is the reduction of tRNA-bound glutamate...

journal_title：Plant molecular biology

authors： Srivastava A,Lake V,Nogaj LA,Mayer SM,Willows RD,Beale SI

更新日期：2005-07-01 00:00:00

abstract：:Vascular tissue in plants is unique due to its diverse and dynamic cellular patterns. Signals controlling vascular development have only recently started to emerge through biochemical, genetic, and genomic approaches in several organisms, such as Arabidopsis, Populus, and Zinnia. These signals include hormones (auxin,...

journal_title：Plant molecular biology

authors： Dettmer J,Elo A,Helariutta Y

更新日期：2009-03-01 00:00:00

abstract：:The barley cysteine proteinase B (EPB) is the main protease responsible for the degradation of endosperm storage proteins providing nitrogenous nutrients to support the growth of young seedlings. The expression of this enzyme is induced in the germinating seeds by the phytohormone, gibberellin, and suppressed by anoth...

journal_title：Plant molecular biology

authors： Mikkonen A,Porali I,Cercos M,Ho TH

更新日期：1996-05-01 00:00:00

abstract：:Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...

journal_title：Plant molecular biology

authors： de Silva J,Robinson SJ,Safford R

更新日期：1992-04-01 00:00:00

abstract：:We have analyzed the nucleotide sequence and accumulation of an mRNA which is prevalent in seeds of Brassica napus L. During normal development, the mRNA begins to accumulate during late embryogeny, is stored in dry seeds, and becomes undetectable in seedlings within 24 hours after imbibition. Moreover, abscisic acid ...

journal_title：Plant molecular biology

authors： Harada JJ,Delisle AJ,Baden CS,Crouch ML

更新日期：1989-04-01 00:00:00

abstract：:Acyl-ACP thioesterases are involved in regulating chain termination of fatty acid biosynthesis in plant systems. Previously, acyl-ACP thioesterase purified from Brassica napus seed tissue has been shown to have a high preference for hydrolysing oleoyl-ACP. Here, oligonucleotides derived from B. napus oleoyl-ACP thioes...

journal_title：Plant molecular biology

authors： Loader NM,Woolner EM,Hellyer A,Slabas AR,Safford R

更新日期：1993-11-01 00:00:00

abstract：:Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

journal_title：Plant molecular biology

authors： Coupe SA,Taylor JE,Isaac PG,Roberts JA

更新日期：1994-01-01 00:00:00

abstract：:CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...

journal_title：Plant molecular biology

authors： Courdavault V,Thiersault M,Courtois M,Gantet P,Oudin A,Doireau P,St-Pierre B,Giglioli-Guivarc'h N

更新日期：2005-04-01 00:00:00

abstract：:The entire mitochondrial genome (mt genome) of the unicellular green alga Platymonas subcordiformis (synonym Tetraselmis subcordiformis; Prasinophyceae) was cloned and a physical map for the four restriction enzymes Hind III, Eco RI, Bg/II and Xba I was constructed. The mt genome of P. subcordiformis is a 42.8 kb circ...

journal_title：Plant molecular biology

authors： Kessler U,Zetsche K

更新日期：1995-12-01 00:00:00

abstract：:Plant S-adenosyl-L-methionine-dependent methyltransferases (SAM-Mtases) are the key enzymes in phenylpropanoid, flavonoid and many other metabolic pathways of biotechnological importance. Here we compiled the amino acid sequences of 56 SAM-Mtases from different plants and performed a computer analysis for the conserve...

journal_title：Plant molecular biology

authors： Joshi CP,Chiang VL

更新日期：1998-07-01 00:00:00

abstract：:Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...

journal_title：Plant molecular biology

authors： Zhao ZY,Cai T,Tagliani L,Miller M,Wang N,Pang H,Rudert M,Schroeder S,Hondred D,Seltzer J,Pierce D

更新日期：2000-12-01 00:00:00

abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...

journal_title：Plant molecular biology

authors： Shen Q,Pu Q,Liang J,Mao H,Liu J,Wang Q

更新日期：2019-08-01 00:00:00