Abstract：
:Plant microRNAs (miRNAs) act as negative regulators of gene expression by slicing target transcripts or inhibiting translation. A number of miRNAs play important roles in development. In order to investigate the potential function of miRNAs during male gametogenesis in rice, we obtained both gene and small RNA expression profiles by combining microarray and high-throughput sequencing technologies. From the microarray datasets, 2,925 male gametophyte-specific genes were identified, including 107 transcription factors and three significant Argonaute genes (AGO12, AGO13, and AGO17). From the sRNA-Seq datasets, 104 unique miRNAs (miRus) were identified, including 47 known miRus and 57 novel miRus; interestingly, most of the new miRus are pollen-specific and not conserved among species. Furthermore, an interactive network of miRNA-target was constructed based on the two datasets. By employing enrichment analysis, the miRNA-regulated targets were found to be involved in both the up and down pathways, but predominantly in the down pathways, including 37 GO biological processes and 32 KEGG pathways. These findings indicate that miRNAs play a broad regulatory role during male gametophyte development in rice.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Peng H,Chun J,Ai TB,Tong YA,Zhang R,Zhao MM,Chen F,Wang SHdoi
10.1007/s11103-012-9898-xsubject
Has Abstractpub_date
2012-09-01 00:00:00pages
85-102issue
1eissn
0167-4412issn
1573-5028journal_volume
80pub_type
杂志文章abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0954-7
更新日期：2005-03-01 00:00:00
abstract：:As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00028892
更新日期：1992-12-01 00:00:00
abstract：:A novel Rab GTPase protein in Arabidopsis thaliana, CPRabA5e (CP = chloroplast localized) is located in chloroplasts and has a role in transport. Transient expression of CPRabA5e:EGFP fusion protein in tobacco (Nicotiana tabacum) leaves, and immunoblotting using Arabidopsis showed localization of CPRabA5e in chloropla...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0161-x
更新日期：2014-04-01 00:00:00
abstract：:Gibberellins (GAs) are important plant growth regulators, regulating many plant developmental processes, including seed germination, root and stem elongation, rosette expansion, floral induction and anther development. The diverse effects of GAs on plant development make it critical to maintain an appropriate endogeno...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-006-9099-6
更新日期：2007-02-01 00:00:00
abstract：:Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9816-7
更新日期：2011-11-01 00:00:00
abstract：:In the post-genomic era, many useful tools have been developed to accelerate the investigation of gene functions. Fluorescent proteins have been widely used as protein tags for studying the subcellular localization of proteins in plants. Several fluorescent organelle marker lines have been generated in dicot plants; h...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-015-0397-8
更新日期：2016-01-01 00:00:00
abstract：:TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1020719420867
更新日期：2003-01-01 00:00:00
abstract：:Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0451-z
更新日期：2005-11-01 00:00:00
abstract：:Two full-length cDNA clones (faEG1 and faEG3, respectively) have been isolated by screening a cDNA library representing transcripts from red strawberry fruits. Southern blot analysis of genomic DNA suggests that the strawberry endo-beta-1,4-glucanases (EGases) are encoded by a multigene family. The cognate genes are p...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006299821980
更新日期：1999-05-01 00:00:00
abstract：:The cytokinin gene, isopentenyl transferase (ipt), was placed under the control of a heat-inducible promoter from the Drosophila melanogaster hsp70 gene and introduced into Nicotiana plumbaginifolia by cocultivation with Agrobacterium tumefaciens. Transformants were analyzed for organ-specific expression, cytokinin le...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017921
更新日期：1991-01-01 00:00:00
abstract：:Knowledge of the genes in gymnosperms encoding the apoproteins of the plant photoreceptor phytochrome is currently scanty as for gymnosperm nuclear protein coding sequences in general. Here we report two complete cDNA-derived sequences which code for two different types of gymnosperm phytochrome. One sequence stems fr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006204318499
更新日期：1999-07-01 00:00:00
abstract：:Differentiation of the Arabidopsis thaliana (Arabidopsis) seed coat epidermal cells involves pronounced changes highlighted by the synthesis and secretion of copious amounts of dispensable, pectinaceous mucilage followed by a thick cellulosic secondary cell wall. This cell type, therefore, represents an excellent mole...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-012-9984-0
更新日期：2013-01-01 00:00:00
abstract：KEY MESSAGE:Progression of the infection canal that conducts rhizobia to the nodule primordium requires a functional Rab GTPase located in Golgi/trans-Golgi that also participate in root hair polar growth. Common bean (Phaseolus vulgaris) symbiotically associates with its partner Rhizobium etli, resulting in the format...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0581-5
更新日期：2017-04-01 00:00:00
abstract：:The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-6235-z
更新日期：2004-12-01 00:00:00
abstract：:Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00016064
更新日期：1984-05-01 00:00:00
abstract：KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-0707-z
更新日期：2018-03-01 00:00:00
abstract：:Two wheat histone H2B genes (TH123 and TH153) were isolated. Nucleotide sequence analysis revealed that some characteristic sequence motifs were conserved in both the 5'- and 3'-flanking regions. A canonical TATA box and several CCAAT sequences were present in the presumed promoter regions. Motifs similar or identical...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042047
更新日期：1995-04-01 00:00:00
abstract：KEY MESSAGE:RNA-seq analysis on whitebark pine needles demonstrated that methyl jasmonate (MeJA)-triggered transcriptome re-programming substantially overlapped with defense responses against insects and fungal pathogens in Pinus species, increasing current knowledge regarding induced systemic resistance (ISR) to patho...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0655-z
更新日期：2017-11-01 00:00:00
abstract：:Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1022502501373
更新日期：2003-03-01 00:00:00
abstract：KEY MESSAGE:Combining with a CRISPR/Cas9 system, Agrobacterium-mediated transformation can lead to precise targeted T-DNA integration in the rice genome. Agrobacterium-mediated T-DNA integration into the plant genomes is random, which often causes variable transgene expression and insertional mutagenesis. Because T-DNA...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-018-00819-1
更新日期：2019-03-01 00:00:00
abstract：:Due to an unfortunate turn of events, the funding note for Open Access publication was not properly provided in the original publication. Hence, the original article has been corrected. The opening line of the Acknowledgement section should read. ...
journal_title：Plant molecular biology
pub_type： 杂志文章,已发布勘误
doi：10.1007/s11103-017-0674-9
更新日期：2017-12-01 00:00:00
abstract：:Using AFLP technology and a recombinant inbred line population derived from the sorghum cross of BTx623 x IS3620C, a high-density genetic map of the sorghum genome was constructed. The 1713 cM map encompassed 2926 loci distributed on ten linkage groups; 2454 of those loci are AFLP products generated from either the Ec...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1014831302392
更新日期：2002-03-01 00:00:00
abstract：:In yeast, Rad21/Scc1 and its meiotic variant Rec8 are key players in the establishment and subsequent dissolution of sister chromatid cohesion for mitosis and meiosis, respectively, which are essential for chromosome segregation. Unlike yeast, our identification revealed that the rice genome has 4 RAD21-like genes tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-4922-z
更新日期：2006-03-01 00:00:00
abstract：:The synthesis, modification, and breakdown of carbohydrates is one of the most fundamentally important reactions in nature. The structural and functional diversity of glycosides is mirrored by a vast array of enzymes involved in their synthesis (glycosyltransferases), modification (carbohydrate esterases) and breakdow...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Acyl-CoA binding proteins (ACBPs) are small (ca. 10 kDa) highly-conserved cytosolic proteins that bind long-chain acyl-CoAs. A novel cDNA encoding ACBP1, a predicted membrane protein of 24.1 kDa with an acyl-CoA binding protein domain at its carboxy terminus, was cloned from Arabidopsis thaliana. At this domain, ACBP1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006052108468
更新日期：1998-11-01 00:00:00
abstract：:The funiculus provides the sole channel of communication between the seed and the parent plant; however, little is known about its role in nutrient supply during seed maturation. Here, we investigated the dynamic metabolite profiles of the funiculus during seed maturation in Brassica napus. The funiculus was fully dev...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0530-3
更新日期：2016-11-01 00:00:00
abstract：:Tree crops have a long juvenile period which is a serious constraint for genetic improvement and experimental research. For example, apple remains in a juvenile phase for more than five years after seed germination. Here, we report about induction of rapid flowering in apple seedlings using the Apple latent spherical ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9718-0
更新日期：2011-01-01 00:00:00
abstract：KEY MESSAGE:KEG is involved in mediating the proteasome-dependent degradation of FDH, a stress-responsive enzyme. The UPS may function to suppress FDH mediated stress responses under favorable growth conditions. Formate dehydrogenase (FDH) has been studied in bacteria and yeasts for the purpose of industrial applicatio...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-017-0691-8
更新日期：2018-02-01 00:00:00
abstract：:A cDNA library was constructed from the root tissues of cassava variety Huanan 124 at the root bulking stage. A total of 9,600 cDNA clones from the library were sequenced with single-pass from the 5'-terminus to establish a catalogue of expressed sequence tags (ESTs). Assembly of the resulting EST sequences resulted i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9698-0
更新日期：2010-12-01 00:00:00
abstract：:Soil contamination by hexavalent chromium [Cr(VI) or chromate] due to anthropogenic activities has become an increasingly important environmental problem. To date few studies have been performed to elucidate the signaling networks involved on adaptive responses to (CrVI) toxicity in plants. In this work, we report tha...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-014-0210-0
更新日期：2014-09-01 00:00:00
