Mutational analysis of pea lectin. Substitution of Asn125 for Asp in the monosaccharide-binding site eliminates mannose/glucose-binding activity.

abstract：:The grp1.8 gene of French bean (Phaseolus vulgaris) is specifically expressed in vascular tissue and encodes a glycine-rich structural protein (GRP1.8) of the cell wall. Earlier promoter analysis had shown that a 28 bp fragment of the grp1.8 promoter (vs-1) confers vascular expression to heterologous minimal promoters...

journal_title：Plant molecular biology

authors： Ringli C,Keller B

更新日期：1998-08-01 00:00:00

abstract：:R2R3-MYB genes play a pivotal role in regulating anthocyanin accumulation. Here, we report two tandemly duplicated R2R3-MYB genes in peach, PpMYB10.1 and PpMYB10.2, with the latter showing lower ability to induce anthocyanin accumulation than the former. Site-directed mutation assay revealed two amino acid changes in ...

journal_title：Plant molecular biology

authors： Zhou H,Liao L,Xu S,Ren F,Zhao J,Ogutu C,Wang L,Jiang Q,Han Y

更新日期：2018-09-01 00:00:00

abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...

journal_title：Plant molecular biology

authors： Turgut K,Barsby T,Craze M,Freeman J,Hodge R,Paul W,Scott R

更新日期：1994-01-01 00:00:00

abstract：:Abscisic acid (ABA) is one of the central plant hormones, responsible for controlling both maturation and germination in seeds, as well as mediating adaptive responses to desiccation, injury, and pathogen infection in vegetative tissues. Thorough analyses of two barley genes, HVA1 and HVA22, indicate that their respon...

journal_title：Plant molecular biology

authors： Ross C,Shen QJ

更新日期：2006-09-01 00:00:00

abstract：:Considerable progress in tomato molecular biology has been made over the past five years. At least 19 different mRNAs which increase in amount during tomato fruit ripening have been cloned and genes for enzymes involved in cell wall degradation (polygalacturonase and pectinesterase) and ethylene synthesis (ACC synthas...

journal_title：Plant molecular biology

authors： Gray J,Picton S,Shabbeer J,Schuch W,Grierson D

更新日期：1992-05-01 00:00:00

abstract：:During early pea fruit growth, the physiological roles of 4-chloroindole-3-acetic acid (4-Cl-IAA) and IAA, natural pea auxins, in regulating gibberellin (GA) 20-oxidase gene expression (PsGA20ox1) were tested with 4-position, ring-substituted auxins that have a range of biological activities (fruit growth). The effect...

journal_title：Plant molecular biology

authors： Ngo P,Ozga JA,Reinecke DM

更新日期：2002-07-01 00:00:00

abstract：:We isolated a rice cDNA encoding nucleoside diphosphate kinase (NDK, EC 2.7.4.6). The deduced amino acid sequence of the rice NDK shows highest homology to spinach NDK-I. The rice NDK gene exhibits a strong codon bias (73.8% GC) in the third position of the codon. DNA blot analysis indicated that at least single NDK g...

journal_title：Plant molecular biology

authors： Yano A,Shimazaki T,Kato A,Umeda M,Uchimiya H

更新日期：1993-12-01 00:00:00

abstract：:In a search for plant genes expressed during early symbiotic interactions between Medicago sativa and Rhizobium meliloti, we have isolated and characterized two alfalfa genes which have strong sequence similarity to members of the Enod12 gene family of Pisum sativum. The M. sativa genes, MsEnod12A and B, encode putati...

journal_title：Plant molecular biology

authors： Allison LA,Kiss GB,Bauer P,Poiret M,Pierre M,Savouré A,Kondorosi E,Kondorosi A

更新日期：1993-01-01 00:00:00

abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...

journal_title：Plant molecular biology

authors： Schuster J,Binder S

更新日期：2005-01-01 00:00:00

abstract：:The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an i...

journal_title：Plant molecular biology

authors： Zhang GZ,Jin SH,Jiang XY,Dong RR,Li P,Li YJ,Hou BK

更新日期：2016-01-01 00:00:00

abstract：:Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...

journal_title：Plant molecular biology

authors： Vogel H,Fischer S,Valentin K

更新日期：1996-11-01 00:00:00

abstract：:A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplas...

journal_title：Plant molecular biology

authors： Paszkowski J,Pisan B,Shillito RD,Hohn T,Hohn B,Potrykus I

更新日期：1986-09-01 00:00:00

abstract：:An 8 Kilobase-pair (Kbp) HindIII fragment containing the coding sequence forSpirulina platensis glutamine synthetase [EC 6.3.1.1.] has been identified utilizing a probe derived fromAnabaena 7120 and cloned in the vector pAT153. ...

journal_title：Plant molecular biology

authors： Riccardi G,De Rossi E,Della Valle G,Ciferri O

更新日期：1985-03-01 00:00:00

abstract：:We report the sequences of full-length cDNAs for the nuclear genes encoding the chloroplastic and cytosolic fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) from spinach. A comparison of the deduced amino-acid sequences with one another and with published cytosolic aldolase sequences of other plants revealed that the ...

journal_title：Plant molecular biology

authors： Pelzer-Reith B,Penger A,Schnarrenberger C

更新日期：1993-01-01 00:00:00

abstract：:In a recent publication (Plant Molecular Biology 16: 547-565 (1991)), Showalter et al. described the isolation and initial characterization of fifteen extensin and extensin-like tomato cDNAs. These cDNAs were determined to fall into five distinct classes; class I and II clones encoded extensins, class III and V clones...

journal_title：Plant molecular biology

authors： Showalter AM,Butt AD,Kim S

更新日期：1992-05-01 00:00:00

abstract：:Chimaeric PCaMV35Scry genes direct in tobacco mesophyll protoplasts mRNA levels of less than one transcript per cell. We provide evidence that this low cytoplasmic cry IA(b) mRNA level is not due to a rapid turnover but rather results from a marginal import flow of cry messenger into the cytoplasm. Run-on assays indic...

journal_title：Plant molecular biology

authors： van Aarssen R,Soetaert P,Stam M,Dockx J,Gosselé V,Seurinck J,Reynaerts A,Cornelissen M

更新日期：1995-06-01 00:00:00

abstract：:Cytosine methylation provides an attractive epigenetic modification for the global maintenance of phases in plant development; however, there are few known examples of specific genes whose methylation status changes in a developmentally regulated manner. Pl-Blotched, an allele of purple plant1 (pl1), which encodes a m...

journal_title：Plant molecular biology

authors： Irish EE,McMurray D

更新日期：2006-03-01 00:00:00

abstract：:We have identified two single-copy genes from the model legume. Medicago truncatula (MtENOD16 and 20) whose expression can be correlated with early stages of root nodulation and whose predicted coding sequences are partially homologous to both pea/vetch ENOD5 and soybean N315/ENOD55. Database searching and sequence al...

journal_title：Plant molecular biology

authors： Greene EA,Erard M,Dedieu A,Barker DG

更新日期：1998-03-01 00:00:00

abstract：KEY MESSAGE:Lack of structural components results in inflorescence stem bending. Differentially expressed genes involved in lignin and hemicellulose biosynthesis are vital; genes involved in cellulose and glycan biosynthesis are also relevant. An erect inflorescence stem is essential for high-quality cut herbaceous peo...

journal_title：Plant molecular biology

authors： Wan Y,Zhang M,Hong A,Lan X,Yang H,Liu Y

更新日期：2020-02-01 00:00:00

abstract：:In previous work, a 33 kDa cysteine proteinase was found in callus initiated from maize (Zea mays L.) resistant to fall armyworm feeding. A callus cDNA library from the maize inbred Mp708 was screened with oligonucleotides derived from the N-terminal amino acid sequence of the 33 kDa proteinase and several cDNA clones...

journal_title：Plant molecular biology

authors： Pechan T,Jiang B,Steckler D,Ye L,Lin L,Luthe DS,Williams WP

更新日期：1999-05-01 00:00:00

abstract：:Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...

journal_title：Plant molecular biology

authors： Takaiwa F,Oono K,Wing D,Kato A

更新日期：1991-10-01 00:00:00

abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...

journal_title：Plant molecular biology

authors： Rawat R,Xu ZF,Yao KM,Chye ML

更新日期：2005-03-01 00:00:00

abstract：:The beneficial root-colonizing fungus Piriformospora indica stimulates root development of Chinese cabbage (Brassica campestris subsp. Chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (GSH)-S-transferase gene (BcGSTU) (Lee et al. 2011) in the roots. BcGSTU expression is further promoted...

journal_title：Plant molecular biology

authors： Kao CW,Bakshi M,Sherameti I,Dong S,Reichelt M,Oelmüller R,Yeh KW

更新日期：2016-12-01 00:00:00

abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...

journal_title：Plant molecular biology

authors： Wu H,Echt CS,Popp MP,Davis JM

更新日期：1997-04-01 00:00:00

abstract：:Pathogenesis related-10 (PR-10) proteins are present as multigene family in most of the higher plants. The role of PR-10 proteins in plant is poorly understood. A sequence analysis revealed that a large number of PR-10 proteins possess conserved motifs found in aldo/keto reductases (AKRs) of yeast and fungi. We took t...

journal_title：Plant molecular biology

authors： Jain D,Khandal H,Khurana JP,Chattopadhyay D

更新日期：2016-01-01 00:00:00

abstract：:We report the successful combination of mRNA differential-display reverse-transcription PCR (DDRT-PCR) and 5'-rapid amplification of cDNA ends (5'-RACE) in order to isolate full-length cDNAs corresponding to genes activated in tobacco cells treated with cryptogein within 60 min. Cloning and sequencing of two cDNAs, ca...

journal_title：Plant molecular biology

authors： Petitot AS,Blein JP,Pugin A,Suty L

更新日期：1997-10-01 00:00:00

abstract：:During flower development, pluripotent stem cells within the floral meristem give rise to proliferative precursor cells whose progeny eventually acquire specialized functions within each floral organ. The regulatory mechanisms by which plant cells transition from a proliferating state to a differentiated state are not...

journal_title：Plant molecular biology

authors： Krizek BA,Eaddy M

更新日期：2012-02-01 00:00:00

abstract：:As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...

journal_title：Plant molecular biology

authors： Eckardt NA,McHenry L,Guiltinan MJ

更新日期：1998-11-01 00:00:00

abstract：:Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...

journal_title：Plant molecular biology

authors： Guo D,Gao X,Li H,Zhang T,Chen G,Huang P,An L,Li N

更新日期：2008-03-01 00:00:00

abstract：:We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (...

journal_title：Plant molecular biology

authors： Li Z,Wurtzel ET

更新日期：1998-07-01 00:00:00