Abstract:
:The formation of auxin glucose conjugate is proposed to be one of the molecular modifications controlling auxin homeostasis. However, the involved mechanisms and relevant physiological significances are largely unknown or poorly understood. In this study, Arabidopsis UGT75D1 was at the first time identified to be an indole-3-butyric acid (IBA) preferring glycosyltransferase. Assessment of enzyme activity and IBA conjugates in transgenic plants ectopically expressing UGT75D1 indicated that the UGT75D1 catalytic specificity was maintained in planta. It was found that the expression pattern of UGT75D1 was specific in germinating seeds. Consistently, we found that transgenic seedlings with over-produced UGT75D1 exhibited smaller cotyledons and cotyledon epidermal cells than the wild type. In addition, UGT75D1 was found to be up-regulated under mannitol, salt and ABA treatments and the over-expression lines were tolerant to osmotic and salt stresses during germination, resulting in an increased germination rate. Quantitative RT-PCR analysis revealed that the mRNA levels of ABA INSENSITIVE 3 (ABI3) and ABI5 gene in ABA signaling were substantially down-regulated in the transgenic lines under stress treatments. Interestingly, AUXIN RESPONSE FACTOR 16 (ARF16) gene of transgenic lines was also dramatically down-regulated under the same stress conditions. Since ARF16 functions as an activator of ABI3 transcription, we supposed that UGT75D1 might play a role in stress tolerance during germination through modulating ARF16-ABI3 signaling. Taken together, our work indicated that, serving as the IBA preferring glycosyltransferase but distinct from other auxin glycosyltransferases identified so far, UGT75D1 might be a very important player mediating a crosstalk between cotyledon development and stress tolerance of germination at the early stage of plant growth.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Zhang GZ,Jin SH,Jiang XY,Dong RR,Li P,Li YJ,Hou BKdoi
10.1007/s11103-015-0395-xsubject
Has Abstractpub_date
2016-01-01 00:00:00pages
77-93issue
1-2eissn
0167-4412issn
1573-5028pii
10.1007/s11103-015-0395-xjournal_volume
90pub_type
杂志文章abstract::Transposable elements were first discovered in plants because they can have tremendous effects on genome structure and gene function. Although only a few or no elements may be active within a genome at any time in any individual, the genomic alterations they cause can have major outcomes for a species. All major eleme...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract:KEY MESSAGE:We reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthases that control the partitioning of precursors for lavender terpenoids. Lavender essential oil is composed of regular and irregular monoterpenes, which are derived from linear precursors geranyl diphosphat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00962-8
更新日期:2020-03-01 00:00:00
abstract::We have isolated cDNA clones encoding the pentose phosphate pathway enzymes 6-phosphogluconate dehydrogenase (6PGDH, EC 1.1.1.44) and glucose 6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) from alfalfa (Medicago sativa L.). These exhibit extensive nucleotide and amino acid sequence similarity to the corresponding gene...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042073
更新日期:1995-08-01 00:00:00
abstract::Gene expression for nuclear- and mitochondrial-encoded subunits of respiratory chain components was measured in developing soybean cotyledons and compared to the abundance of the relevant proteins. Overall respiratory gene expression peaked at day 16, close to the peak in cytochrome chain and TCA cycle activities from...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1022502501373
更新日期:2003-03-01 00:00:00
abstract::Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for C(4) photosynthesis, providing the primary acceptor for fixation of bicarbonate in mesophyll cells. Although first isolated in C(4) plants, it is also present in C(3) species. We report that the single gene encoding PPDK in Arabidopsis thaliana possesses...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9023-0
更新日期:2006-10-01 00:00:00
abstract::Tremendous efforts have been taken worldwide to develop genome-wide genetic stocks for rice functional genomic (FG) research since the rice genome was completely sequenced. To facilitate FG research of complex polygenic phenotypes in rice, we report the development of over 20,000 introgression lines (ILs) in three eli...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-8519-3
更新日期:2005-09-01 00:00:00
abstract::The full-length nucleotide sequence of a barley (Hordeum vulgare L.) leaf mRNA, found to increase rapidly in amount during infection attempts by the powdery mildew fungus (Erysiphe graminis DC. ex Mérat), is reported. The mRNA encodes a polypeptide of 809 amino acid residues which, by sequence comparison, was identifi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023606
更新日期:1993-03-01 00:00:00
abstract::TGA1a is a well-characterized transcription factor that may mediate the root-specific and auxin-responsive expression of some plant genes. In tobacco, Southern blot and genomic cloning analyses have shown that TGA1a consists of at least four closely related genes. Since TGA1a belongs to the bZIP class of transcription...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024193
更新日期:1994-04-01 00:00:00
abstract:KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01037-4
更新日期:2020-09-01 00:00:00
abstract::The GSK3/Shaggy family of serine/threonine protein kinases is involved in a series of biological processes in animals, plants and yeast [Charrier et al. (2002) Plant Physiol 130:577-590; Jope and Johnson (2004) Trends Biochem Sci 29:95-102; Li and Nam (2002) Science 295:1299-1301; Piao et al. (2001) Plant J 27:305-314...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9138-y
更新日期:2007-05-01 00:00:00
abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043873
更新日期:1994-06-01 00:00:00
abstract::The nucleotide sequences of the class I phycoerythrin (PE) alpha- and beta-subunit genes (cpeA and cpeB) from the marine cyanobacterium Synechococcus sp. WH7803 are reported. The cpeB gene is located upstream of cpeA with a separation of 56 nucleotides and the two genes are co-transcribed as a transcript of 1.3 kb, wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023564
更新日期:1994-02-01 00:00:00
abstract::Persian walnut (Juglans regia L.) is an economically important tree for its nut crop and timber. To gain insight into the structure and evolution of the walnut genome, we constructed two bacterial artificial chromosome (BAC) libraries, containing a total of 129,024 clones, from in vitro-grown shoots of J. regia cv. Ch...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9849-y
更新日期:2012-01-01 00:00:00
abstract::Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020209
更新日期:1996-11-01 00:00:00
abstract::RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037063
更新日期:1991-10-01 00:00:00
abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019520
更新日期:1988-11-01 00:00:00
abstract::Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015816
更新日期:1987-09-01 00:00:00
abstract::Arabidopsis is currently the reference genome for higher plants. A new, more detailed statistical analysis of Arabidopsis gene structure is presented including intron and exon lengths, intergenic distances, features of promoters, and variant 5'-ends of mRNAs transcribed from the same transcription unit. We also provid...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2564-9
更新日期:2006-01-01 00:00:00
abstract::Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00166458
更新日期:1987-05-01 00:00:00
abstract::The identification of a cDNA (MR19) corresponding to a maize alpha-tubulin and homologous genomic clones (MG19/6 and MG19/14) is described. The cDNA has been isolated by differential screening of a cDNA maize root library. We have found two alpha-tubulin genes in a tandem arrangement in the genomic clones, separated b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015650
更新日期:1990-01-01 00:00:00
abstract::The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418259
更新日期:1985-11-01 00:00:00
abstract::Immunofluorescence flow cytometry was used to study the distribution of viral antigen in protoplast populations. Protoplasts were isolated from healthy and alfalfa mosaic virus (AMV) infected tobacco leaves (designated in vivo infected). Furthermore isolated tobacco and cowpea protoplasts were infected in vitro with A...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00187572
更新日期:1983-01-01 00:00:00
abstract::MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0156-7
更新日期:2014-03-01 00:00:00
abstract::Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9229-9
更新日期:2007-11-01 00:00:00
abstract::Microtubules are thought to be major determinants of plant morphogenesis, through effects on planes of cell division and on directions of differential cell expansion. In differentiation and redifferentiation processes, tubulin expression may prove a useful early indicator of cell activity. We examined the expression a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006338401808
更新日期:1999-11-01 00:00:00
abstract::Binary Ti vectors are the plasmid vectors of choice in Agrobacterium-mediated plant transformation protocols. The pGreen series of binary Ti vectors are configured for ease-of-use and to meet the demands of a wide range of transformation procedures for many plant species. This plasmid system allows any arrangement of ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006496308160
更新日期:2000-04-01 00:00:00
abstract::Developmentally regulated GTP-binding proteins (DRGs) from animals and fungi are highly conserved but have no known function. Here we characterize DRGs from pea (PsDRG) and Arabidopsis (AtDRG). Amino acid sequences of AtDRG and PsDRG were 90% identical to each other and about 65% identical to human DRG. Genomic Southe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006178710443
更新日期:1999-01-01 00:00:00
abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017466
更新日期:1988-09-01 00:00:00
abstract:KEY MESSAGE:Hybrid abnormalities, severe growth abortion and grass-clump dwarfism, were found in the tetraploid wheat/Aegilops umbellulata hybrids, and the gene expression changes were conserved in the hybrids with those in other wheat synthetic hexaploids. Aegilops umbellulata Zhuk., a diploid goatgrass species with a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0677-6
更新日期:2017-12-01 00:00:00