Abstract:
:Three polypeptides of 33 kD, 23 kD and 16 kD are released from maize photosystem II particles by alkaline Tris solution treatment and shown to cross-react with antisera to purified spinach oxygen-evolving (OE) polypeptides of 34 kD, 23 kD and 17 kD, respectively. They are not located exclusively in mesophyll cells but each is about 4 times more abundant in the thylakoid membranes of mesophyll than bundle sheath cells of etiolated, greening and green leaves. Three maize cDNA clones (OE33, OE23, OE16) have been identified by hybrid-selection, in vitro translation and immunoprecipitation with antisera against spinach OEs. Transcripts of all three genes are already detectable in both mesophyll and bundle sheath cells of etiolated leaves; however, they accumulate transiently and coordinately in mesophyll cells but remain at a constant low level in bundle sheath cells upon illumination of dark-grown maize seedlings. Moreover, the level of each protein increases in mesophyll cells following the accumulation of transcripts during greening and remains high in late greening and green leaves, despite the decline in each corresponding mRNA. The accumulation of all three OE proteins is also stimulated by light in bundle sheath cells without increases in their corresponding mRNAs. The preferential localization of these three proteins in mesophyll cells is due to both transcriptional and translational regulation.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Sheen JY,Sayre RT,Bogorad Ldoi
10.1007/BF00166458subject
Has Abstractpub_date
1987-05-01 00:00:00pages
217-26issue
3eissn
0167-4412issn
1573-5028journal_volume
9pub_type
杂志文章abstract:KEY MESSAGE:The induction of miR399 and miR398 and the inhibition of miR156, miR159, miR160, miR171, miR2111, and miR2643 were observed under Pi deficiency in alfalfa. The miRNA-mediated genes involved in basic metabolic process, root and shoot development, stress response and Pi uptake. Inorganic phosphate (Pi) defici...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0711-3
更新日期:2018-03-01 00:00:00
abstract::The variation in grain hardness is the single most important trait that determines end-use quality of wheat. Grain texture classification is based primarily on either the resistance of kernels to crushing or the particle size distribution of ground grain or flour. Recently, the molecular genetic basis of grain hardnes...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1014837431178
更新日期:2002-03-01 00:00:00
abstract::Three genes that encode MAP65-1 family proteins have been identified in the Nicotiana tabacum genome. In this study, NtMAP65-1c fusion protein was shown to bind and bundle microtubules (MTs). Further in vitro investigations demonstrated that NtMAP65-1c not only alters MT assembly and nucleation, but also exhibits high...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9694-4
更新日期:2010-12-01 00:00:00
abstract::CaaX-prenyltransferases (CaaX-PTases) catalyse the covalent attachment of isoprenyl groups to conserved cysteine residues located at the C-terminal CaaX motif of a protein substrate. This post-translational modification is required for the function and/or subcellular localization of some transcription factors and comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-3095-0
更新日期:2005-04-01 00:00:00
abstract::A polymerase chain reaction product (PKIN503) was amplified from potato (Solanum tuberosum) cv. Désirée using oligonucleotide primers with sequences which are highly conserved in the plant sucrose non-fermenting 1 (SNF1)-related protein kinase gene family. Southern blot analysis showed the presence of 5-10 SNF1-relate...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005765719873
更新日期:1997-05-01 00:00:00
abstract::Complementary DNA clones encoding acidic and basic isoforms of the class III chitinase were isolated from Nicotiana tabacum. The clones share ca. 65% identity, are equally homologous to the class III chitinases from cucumber and Arabidopsis, and are members of small gene families in tobacco. An acidic class III chitin...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027070
更新日期:1992-08-01 00:00:00
abstract::The location on the wheat chloroplast DNA map and the nucleotide sequences of the genes coding for tRNA GCC (Gly) (trnG-GCC), tRNA GUC (Asp) (trnD-GUC) and tRNA GCA (Cys) (trnC-GCA) have been determined. These three genes are located in the large single copy region of the chloroplast genome, about half-way between one...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418249
更新日期:1985-09-01 00:00:00
abstract::A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020195
更新日期:1995-01-01 00:00:00
abstract::Dehydrins are characterized by the consensus KIKEKLPG amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. A synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. T...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00029004
更新日期:1993-10-01 00:00:00
abstract::The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00025326
更新日期:1987-03-01 00:00:00
abstract::GRAS proteins belong to a plant-specific transcription factor family. Currently, 33 GRAS members including a putative expressed pseudogene have been identified in the Arabidopsis genome. With a reverse genetic approach, we have constructed a "phenome-ready unimutant collection" of the GRAS genes in Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9345-1
更新日期:2008-08-01 00:00:00
abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016047231597
更新日期:2002-09-01 00:00:00
abstract::The function of the secretory pathway is dependent on multiple protein-protein interactions at various stages. Currently, such interactions are mainly studied using physical methods that document direct contact or affinity in vitro. The development of vital fluorescence imaging as well as quantitative protein transpor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1023/a:1021266320877
更新日期:2002-12-01 00:00:00
abstract:KEY MESSAGE:IEF, a novel plasma plasma membrane protein, is important for exine formation in Arabidopsis. Exine, an important part of pollen wall, is crucial for male fertility. The major component of exine is sporopollenin which are synthesized and secreted by tapetum. Although sporopollenin synthesis has been well st...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01114-8
更新日期:2021-01-22 00:00:00
abstract::The root endophytic fungus Piriformospora indica enhances plant adaptation to environmental stress based on general and non-specific plant species mechanisms. In the present study, we integrated the ionomics, metabolomics, and transcriptomics data to identify the genes and metabolic regulatory networks conferring salt...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0461-z
更新日期:2016-04-01 00:00:00
abstract::Repetitive DNA sequences were detected directly on somatic metaphase chromosome spreads from soybean root tips using fluorescent in situ hybridization. Methods to spread the forty small metaphase chromosomes substantially free of cellular material were developed using protoplasts. The specific DNA probe was a 1.05 kb ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00036810
更新日期:1991-07-01 00:00:00
abstract::The gene encoding nitrite reductase (nir) from the cyanobacterium Synechococcus sp. PCC 7942 has been identified and sequenced. This gene comprises 1536 nucleotides and would encode a polypeptide of 56,506 Da that shows similarity to nitrite reductase from higher plants and to the sulfite reductase hemoprotein from en...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023618
更新日期:1993-03-01 00:00:00
abstract::We have cloned and sequenced a wound-inducible cDNA clone designated WIP1 (for wound-induced protein) from maize coleoptiles. It was isolated by differential screening of a cDNA library prepared from excised maize coleoptile segments. The deduced amino acid sequence predicts a secretory, cysteine-rich protein of 102 r...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027365
更新日期:1993-08-01 00:00:00
abstract::Expression of the light-harvesting complex protein genes (Lhc) is under the control of a circadian clock. To dissect the molecular regulatory components of the circadian clock a promoter deletion analysis of four tomato Lhc genes was performed in transgenic tobacco plants. The important 5'-upstream promoter regions ar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006094015513
更新日期:1998-11-01 00:00:00
abstract::Acyl carrier protein (ACP) is a key component of the fatty acid biosynthetic machinery in plants. A 1.4 kb 5' flanking region of a Brassica napus ACP gene (ACP05) was transcriptionally fused to the reporter gene beta-glucuronidase (GUS), and expression of the chimeric gene monitored in transgenic tobacco. GUS activity...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00047719
更新日期:1992-04-01 00:00:00
abstract::High molecular weight double-stranded (ds) RNAs have been detected in apparently virus-free French (common) bean Phaseolus vulgaris cv. Black Turtle Soup (BTS). Several other bean cultivars were free of detectable high molecular weight dsRNAs. The dsRNAs have been partially characterized and have homology to the BTS g...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017873
更新日期:1985-01-01 00:00:00
abstract::The expression of chloroplast genes during cotton embryogenesis was followed by measuring hybridization of cloned chloroplast genomic fragments to total RNA immobilzed on membrane filters. RNA was extracted from the cotyledons of cotton embryos at different stages of development ranging from very small embryos (5 mg) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040137
更新日期:1986-03-01 00:00:00
abstract::We have determined the nucleotide sequence of two small circular DNA plasmids, pCf1 and pCf2 [22], from the marine diatom Cylindrotheca fusiformis. pCf1 is 4273 bp, and pCf2 is 4079 bp in size. In each plasmid, all of the major open reading frames (ORFs) are encoded on the same DNA strand. Two ORFs are similar, compar...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027072
更新日期:1992-08-01 00:00:00
abstract::Phaseolin is a glycoprotein that constitutes the major storage protein in bean seeds. The phaseolin gene promoters function in a seed-specific manner. In an attempt to understand if events following transcription of the gene also contribute to the seed-specific accumulation of the phaseolin protein, we studied the eff...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040527
更新日期:1992-09-01 00:00:00
abstract::We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of L...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040674
更新日期:1992-02-01 00:00:00
abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020464
更新日期:1995-12-01 00:00:00
abstract::We used a functional screening method to isolate genes whose products elicit the hypersensitive response (HR) pathway of defense against plant pathogens. A cDNA library derived from tobacco leaves undergoing the HR was cloned into a tobacco mosaic virus (TMV)-based expression vector. Infectious transcripts were genera...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005949304445
更新日期:1998-03-01 00:00:00
abstract::Recent studies with dicot plants reveal that floral organ development is controlled by a group of regulatory factors containing the MADS domain. In this study, we have isolated and characterized a cDNA clone from rice, OsMADS1, which encodes a MADS-domain-containing protein. The OsMADS1 amino acid sequence shows 56.2%...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00013751
更新日期:1994-10-01 00:00:00
abstract:KEY MESSAGE:Cloning of two isoamylase genes, MeISA1 and MeISA2, from cassava (Manihot esculenta Crantz) tubers, accompanied by their co-expression in E. coli demonstrates a requirement for heteromeric complex formation to achieve debranching activity. Starch debranching enzyme (DBE) or isoamylase (ISA) (EC.3.2.1.68), a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0707-z
更新日期:2018-03-01 00:00:00
abstract:KEY MESSAGE:Psoralen synthase and angelicin synthase responsible for the formation of psoralen and angelicin in Peucedanum praeruptorum Dunn were identified and functionally characterized, respectively. Furanocoumarins were reported to possess several activities such as anticancer, anti-inflammatory and neuroprotective...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-01045-4
更新日期:2020-10-01 00:00:00