Structure and expression of the barley lipid transfer protein gene Ltp1.

Abstract:

:We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5' upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Skriver K,Leah R,Müller-Uri F,Olsen FL,Mundy J

doi

10.1007/BF00040674

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

585-9

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

18

pub_type

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