Abstract:
:We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located upstream of the nad3 locus in O. berteriana. The rps14 locus is present as a single copy in the A. thaliana mt genome and has a translational stop codon located near the initiation codon, as well as a deletion of one nucleotide that disturbs the coding sequence. The cloning and sequencing of nine amplified mt rps14 cDNAs clearly demonstrated that this gene is transcribed and that the mRNA precursors are edited at three positions, all involving C-to-U conversions. No editing events changing the stop codon and restoring the correct coding sequence were witnessed within the 9 individual cDNA clones. Therefore, we conclude that the single rps14 sequence of the mitochondrial genome from A. thaliana is in fact a pseudogene that is transcribed and edited but not translated.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Aubert D,Bisanz-Seyer C,Herzog Mdoi
10.1007/BF00028903subject
Has Abstractpub_date
1992-12-01 00:00:00pages
1169-74issue
6eissn
0167-4412issn
1573-5028journal_volume
20pub_type
杂志文章abstract::The rice blast resistance gene Pib is a member of the nucleotide binding site (NBS) and leucine-rich repeat (LRR) class of plant disease resistance (R) genes and belongs to a small gene family. We describe here the isolation and characterization of a Pib homologue (PibH8), and extensive investigation of the expression...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1012457113700
更新日期:2001-11-01 00:00:00
abstract::The transcription factor ABA-Insensitive5 (ABI5) is a key regulator of ABA signaling and stress response in Arabidopsis seeds and seedlings. Potential ABI5-interacting proteins were identified by a yeast two-hybrid screen; the most prevalent interactors were a family of four highly conserved plant-specific proteins wi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9344-2
更新日期:2008-08-01 00:00:00
abstract::The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-7820-x
更新日期:2005-02-01 00:00:00
abstract::Due to an unfortunate turn of events, an incorrect note was provided in the original publication as it should have read. ...
journal_title:Plant molecular biology
pub_type: 已发布勘误
doi:10.1007/s11103-020-00970-8
更新日期:2020-03-01 00:00:00
abstract::Higher-plant, green and red algal chloroplasts are surrounded by a double membrane envelope. The glaucocystophyte plastid (cyanelle) has retained a prokaryotic cell wall between the two envelope membranes. The complex chloroplasts of Euglena and dinoflagellates are surrounded by three membranes while the complex chlor...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1998-09-01 00:00:00
abstract::The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037145
更新日期:1991-11-01 00:00:00
abstract::Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9786-9
更新日期:2011-08-01 00:00:00
abstract::The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019218
更新日期:1992-03-01 00:00:00
abstract::The entire mitochondrial genome (mt genome) of the unicellular green alga Platymonas subcordiformis (synonym Tetraselmis subcordiformis; Prasinophyceae) was cloned and a physical map for the four restriction enzymes Hind III, Eco RI, Bg/II and Xba I was constructed. The mt genome of P. subcordiformis is a 42.8 kb circ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00014979
更新日期:1995-12-01 00:00:00
abstract::Homologous recombination is an essential process for the maintenance and variability of the genome. In eukaryotes, the Rad52 epistasis group proteins serve the main role for meiotic recombination and/or homologous recombinational repair. Rad51-like proteins, such as Rad55 and Rad57 in yeast, play a role in assembly or...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1016047231597
更新日期:2002-09-01 00:00:00
abstract::A genomic library was prepared from Populus kitakamiensis and screened with the cDNA for an anionic peroxidase from P. kitakamiensis. One genomic clone was isolated that contained two tandemly oriented genes for anionic peroxidases, prxA3a and prxA4a. Both genes consisted of four exons and three introns; the introns h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00021193
更新日期:1995-07-01 00:00:00
abstract::TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1020719420867
更新日期:2003-01-01 00:00:00
abstract::A gene encoding the large subunit of ribulose-1,5-bisphosphate carboxylase (Rubisco) was identified on a circular 73 kb DNA from the colourless euglenoid flagellate Astasia longa. The rbcL gene of Astasia extends over 3968 bp. It is a split gene interrupted by seven introns as compared to nine intervening sequences in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016515
更新日期:1990-05-01 00:00:00
abstract::The chromatin structure of pea (Pisum sativum) rbcS genes in inactive (root), potentially active (dark-grown leaf), and active states (light-grown leaf) was analysed using (a) pancreatic DNAseI to detect general DNAseI sensitivity and DNAseI-hypersensitive sites, and (b) methyl-sensitive restriction endonucleases to p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017456
更新日期:1988-09-01 00:00:00
abstract::We identified a novel nucleoplasm localized protein in Arabidopsis called AT-hook motif nuclear localized protein 1 (AHL1), which was isolated by visual screening of transformants using random GFP::cDNA fusions. AHL1 contains an AT-hook motif and unknown conserved PPC (plants and prokaryotes conserved) domain that inc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-3249-5
更新日期:2004-09-01 00:00:00
abstract::Peach fruits subjected to prolonged cold storage (CS) to delay decay and over-ripening often develop a form of chilling injury (CI) called mealiness/woolliness (WLT), a flesh textural disorder characterized by lack of juiciness. Transcript profiles were analyzed after different lengths of CS and subsequent shelf life ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0526-z
更新日期:2016-11-01 00:00:00
abstract::Accumulation and stability of psbA and rbcL-S transcripts in Synechocystis 6803 was followed in the presence and absence of the photosynthesis inhibitors DCMU and methylviologen. Our results demonstrate that both transcript production and transcript stability are important regulatory elements for psbA gene expression ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015080
更新日期:1991-05-01 00:00:00
abstract::Methoxypyrazines (MPs) are volatile, grape-derived aroma compounds that contribute to the distinct herbaceous characters of some wines. Although the full pathway leading to MP production has not been elucidated, there is strong evidence that the final step involves the methylation of non-volatile hydroxypyrazine (HP) ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9655-y
更新日期:2010-09-01 00:00:00
abstract::Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019506
更新日期:1994-12-01 00:00:00
abstract:KEY MESSAGE:This review contains functional roles of MYB transcription factors in the transcriptional regulation of anthocyanin biosynthesis in horticultural plants. This review describes potential uses of MYB TFs as tools for metabolic engineering for anthocyanin production. Anthocyanins (ranging from red to blue) are...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-018-0771-4
更新日期:2018-09-01 00:00:00
abstract::Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006176819213
更新日期:1999-03-01 00:00:00
abstract::Culm mechanical strength is an important agronomic trait in crop breeding. To understand the molecular mechanisms that control culm mechanical strength, we identified a flexible culm1 (fc1) mutant by screening a rice T-DNA insertion mutant library. This mutant exhibited an abnormal development phenotype, including lat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9448-8
更新日期:2009-04-01 00:00:00
abstract::Agrobacterium tumefaciens was used to genetically transform sorghum. Immature embryos of a public (P898012) and a commercial line (PHI391) of sorghum were used as the target explants. The Agrobacterium strain used was LBA4404 carrying a 'Super-binary' vector with a bar gene as a selectable marker for herbicide resista...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026507517182
更新日期:2000-12-01 00:00:00
abstract::A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015653
更新日期:1990-01-01 00:00:00
abstract::APETALA2 (AP2) transcription factors (TFs) play very important roles in plant growth and development and in defense response. Here, we report functional characterization of four AP2 TF family genes [(RAP2.6 (At1g43160), RAP2.6L (At5g13330), DREB 26 (At1g21910) and DREB19 (At2g38340)] that were identified among NaCl in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-010-9711-7
更新日期:2011-01-01 00:00:00
abstract::A three-dimensional model of curculin, a sweet-tasting and taste-modifying protein from the fruits of Curculigo latifolia, was built from the X-ray coordinates of GNA, a mannose-binding lectin from snowdrop (Galanthus nivalis). The three mannose-binding sites present in GNA were found in curculin but are devoid of man...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005704616565
更新日期:1997-03-01 00:00:00
abstract::Infection of tobacco by tobacco mosaic virus (TMV) induces coordinate expression of genes encoding acidic and basic beta-1,3-glucanase isoforms. These genes are differentially expressed in response to other treatments. Salicylate treatment induces acidic glucanase mRNA to a higher level than basic glucanase mRNA. Ethy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028803
更新日期:1993-02-01 00:00:00
abstract::The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017466
更新日期:1988-09-01 00:00:00
abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005708611020
更新日期:1997-04-01 00:00:00
abstract:KEY MESSAGE:Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls. Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor,...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0702-4
更新日期:2018-04-01 00:00:00