The group I intron of apocytochrome b gene from Chlamydomonas smithii encodes a site-specific endonuclease.

Abstract:

:The mitochondrial DNA molecules of two interfertile algal species, Chlamydomonas smithii and C. reinhardtii, are co-linear except for a 1075 bp intron (the alpha-insert) that is present in the cob gene of C. smithii. The alpha-insert, a group I intron (Cs cob.1) containing an open reading frame (ORF) which encodes a basic, hydrophilic protein of 237 amino acids, is unidirectionally transmitted to all diploid progeny during interspecific crosses. In this report, we show that the Cs cob.1-encoded protein is a site-specific endonuclease (I-Csm I) which could mediate the intron transfer via the gene conversion mechanism. The Cs cob.1 ORF was cloned into the vector pMALcr1 and over-expressed as a hybrid protein fused to maltose-binding protein (MBP). This fusion protein exhibited an in vivo endonuclease activity which specifically cleaved the intron homing site within the intronless cob gene.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Ma DP,King YT,Kim Y,Luckett WS Jr

doi

10.1007/BF00019218

subject

Has Abstract

pub_date

1992-03-01 00:00:00

pages

1001-4

issue

5

eissn

0167-4412

issn

1573-5028

journal_volume

18

pub_type

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