Comparative analysis of chrysanthemum transcriptome in response to three RNA viruses: Cucumber mosaic virus, Tomato spotted wilt virus and Potato virus X.

Abstract:

:The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic virus (CMV), Tomato spotted wilt virus (TSWV) and Potato virus X (PVX). A chrysanthemum 135K microarray derived from expressed sequence tags was successfully applied for the expression profiles of the chrysanthemum at early stage of virus infection. Finally, we identified a total of 125, 70 and 124 differentially expressed genes (DEGs) for CMV, TSWV and PVX, respectively. Many DEGs were virus specific; however, 33 DEGs were commonly regulated by three viruses. Gene ontology (GO) enrichment analysis identified a total of 132 GO terms, and of them, six GO terms related stress response and MCM complex were commonly identified for three viruses. Several genes functioning in stress response such as chitin response and ethylene mediated signaling pathway were up-regulated indicating their involvement in establishment of host immune system. In particular, TSWV infection significantly down-regulated genes related to DNA metabolic process including DNA replication, chromatin organization, histone modification and cytokinesis, and they are mostly targeted to nucleosome and MCM complex. Taken together, our comparative transcriptome analysis revealed several genes related to hormone mediated viral stress response and DNA modification. The identified chrysanthemums genes could be good candidates for further functional study associated with resistant to various plant viruses.

journal_name

Plant Mol Biol

journal_title

Plant molecular biology

authors

Choi H,Jo Y,Lian S,Jo KM,Chu H,Yoon JY,Choi SK,Kim KH,Cho WK

doi

10.1007/s11103-015-0317-y

subject

Has Abstract

pub_date

2015-06-01 00:00:00

pages

233-48

issue

3

eissn

0167-4412

issn

1573-5028

journal_volume

88

pub_type

杂志文章
  • CYCLIN-DEPENDENT KINASE G2 regulates salinity stress response and salt mediated flowering in Arabidopsis thaliana.

    abstract::Cyclin-dependent protein kinases are involved in many crucial cellular processes and aspects of plant growth and development, but their precise roles in abiotic stress responses are largely unknown. Here, Arabidopsis thaliana CYCLIN-DEPENDENT KINASE G2 (CDKG2) was shown to act as a negative regulator of the salinity s...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-015-0324-z

    authors: Ma X,Qiao Z,Chen D,Yang W,Zhou R,Zhang W,Wang M

    更新日期:2015-06-01 00:00:00

  • Molecular and structural characterization of barley vernalization genes.

    abstract::Vernalization, the requirement of a period of low temperature to induce transition from the vegetative to reproductive state, is an evolutionarily and economically important trait in the Triticeae. The genetic basis of vernalization in cultivated barley (Hordeum vulgare subsp. vulgare) can be defined using the two-loc...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-0351-2

    authors: von Zitzewitz J,Szucs P,Dubcovsky J,Yan L,Francia E,Pecchioni N,Casas A,Chen TH,Hayes PM,Skinner JS

    更新日期:2005-10-01 00:00:00

  • Chloroplast transcription is required to express the nuclear genes rbcS and cab. Plastid DNA copy number is regulated independently.

    abstract::RbcL and rbcS mRNA levels and plastid transcription activity are low in the basal meristematic region of barley primary leaves and increase coordinately during leaf cell development with a similar time course in dark-grown or illuminated seedlings. The capacity of light to cause cab mRNA accumulation shows a similar d...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00037063

    authors: Rapp JC,Mullet JE

    更新日期:1991-10-01 00:00:00

  • Characterization of a mRNA that accumulates during development of oilseed rape pods.

    abstract::Dehiscence of oilseed rape pods, commonly known as pod shatter, is a process of agronomic importance that results in seed loss causing yield reductions and carry-over of the crop into the following growing season. In an effort to understand the mechanisms underlying this developmental event, the changes in gene expres...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00040589

    authors: Coupe SA,Taylor JE,Isaac PG,Roberts JA

    更新日期:1994-01-01 00:00:00

  • GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress.

    abstract::Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor composed of NF-YA, NF-YB and NF-YC proteins. In this study, we identified and characterized a gene, GmNFYA3, which encodes the NF-YA subunit of the NF-Y complex in soybeans (Glycine max L.). Real time RT-PCR analysis indicated that GmNFYA3 was induced by...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-013-0040-5

    authors: Ni Z,Hu Z,Jiang Q,Zhang H

    更新日期:2013-05-01 00:00:00

  • AINTEGUMENTA-LIKE6 regulates cellular differentiation in flowers.

    abstract::During flower development, pluripotent stem cells within the floral meristem give rise to proliferative precursor cells whose progeny eventually acquire specialized functions within each floral organ. The regulatory mechanisms by which plant cells transition from a proliferating state to a differentiated state are not...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-011-9844-3

    authors: Krizek BA,Eaddy M

    更新日期:2012-02-01 00:00:00

  • An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases.

    abstract::The regulation of genes in response to wounding is mediated in part by the octadecanoids 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA) and its methyl ester methyl jasmonate (MeJA). We identified, by differential display, an Arabidopsis gene (OPR3) induced after wounding. OPR3 is homologous to members of the flav...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006464822434

    authors: Costa CL,Arruda P,Benedetti CE

    更新日期:2000-09-01 00:00:00

  • Differential expression profiles of growth-related genes in the elongation zone of maize primary roots.

    abstract::Growth in the apical elongation zone of plant roots is central to the development of functional root systems. Rates of root segmental elongation change from accelerating to decelerating as cell development proceeds from newly formed to fully elongated status. One of the primary variables regulating these changes in el...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-004-3474-y

    authors: Bassani M,Neumann PM,Gepstein S

    更新日期:2004-10-01 00:00:00

  • Overexpression of deltaEmBP, a truncated dominant negative version of the wheat G-box binding protein EmBP-1, alters vegetative development in transgenic tobacco.

    abstract::As a first step toward elucidating the in vivo function of plant bZIP proteins and their related G-box cis elements, we have introduced a dominant negative inhibitor of G-box-dependent transcriptional activation into tobacco plants by transforming them with a truncated EmBP-1 gene (deltaEmBP) containing the DNA bindin...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006081009173

    authors: Eckardt NA,McHenry L,Guiltinan MJ

    更新日期:1998-11-01 00:00:00

  • Chemical synthesis, expression and mutagenesis of a gene encoding beta-cryptogein, an elicitin produced by Phytophthora cryptogea.

    abstract::Elicitins are 10 kDa holoproteins secreted by Phytophthora fungi, that elicit an incompatible hypersensitive reaction, leading to resistance against fungal and bacterial plant pathogens. Comparison of primary sequences of alpha-elicitins and beta-elicitins indicated several potential necrotic activity-determining resi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019323

    authors: O'Donohue MJ,Gousseau H,Huet JC,Tepfer D,Pernollet JC

    更新日期:1995-02-01 00:00:00

  • Lignin synthesized by CmCAD2 and CmCAD3 in oriental melon (Cucumis melo L.) seedlings contributes to drought tolerance.

    abstract:KEY MESSAGE:CmCAD2 and CmCAD3 function more positively than CmCAD1 in oriental melon for lignin synthesis which is important to ensure internal water status and thus for drought tolerance. Well-lignification may be the guarantee of efficient axial water transport and barrier of lateral water flow in oriental melon tole...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01018-7

    authors: Liu W,Jiang Y,Wang C,Zhao L,Jin Y,Xing Q,Li M,Lv T,Qi H

    更新日期:2020-08-01 00:00:00

  • Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric viral DNA.

    abstract::Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Ag...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019520

    authors: Morris BA,Richardson KA,Andersen MT,Gardner RC

    更新日期:1988-11-01 00:00:00

  • Context sequences of translation initiation codon in plants.

    abstract::In this survey of 5074 plant genes for their AUG context sequences, purines are present at the -3 and +4 positions in about 80% of the sequences. Although this observation is similar to the vertebrate consensus sequence, the number of plant mRNAs with purines at the -3 position is lower and at the +4 position is highe...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1005816823636

    authors: Joshi CP,Zhou H,Huang X,Chiang VL

    更新日期:1997-12-01 00:00:00

  • Molecular characterization of glyoxalase-I from a higher plant; upregulation by stress.

    abstract::A cDNA, GLX1, encoding glyoxalase-I was isolated by differential screening of salt-induced genes in tomato. Glyoxalases-I and -II are ubiquitous enzymes whose functions are not clearly understood. They may serve to detoxify methylglyoxal produced from triosephosphates in all cells. The protein encoded by GLX1 shared 4...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020464

    authors: Espartero J,Sánchez-Aguayo I,Pardo JM

    更新日期:1995-12-01 00:00:00

  • Isolation of a novel Arabidopsis ozone-induced cDNA by differential display.

    abstract::We have used a reverse transcriptase polymerase chain reaction procedure (differential display) to isolate cDNAs corresponding to transcripts that accumulate in ozone-treated Arabidopsis thaliana. In this report we describe the characterization of an ozone-induced transcript, AtOZI1. AtOZI1 mRNA in untreated plants wa...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00019121

    authors: Sharma YK,Davis KR

    更新日期:1995-10-01 00:00:00

  • Mitochondrial rps14 is a transcribed and edited pseudogene in Arabidopsis thaliana.

    abstract::We have isolated and analysed a 2 kb region of the mitochondrial genome of Arabidopsis thaliana (Columbia) showing a high level of nucleotide identity with the mitochondrial (mt) rps14 small-subunit ribosomal protein gene from Oenothera berteriana and Vicia faba, as well as with an open reading frame (ORF) located ups...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00028903

    authors: Aubert D,Bisanz-Seyer C,Herzog M

    更新日期:1992-12-01 00:00:00

  • An OGG1 orthologue encoding a functional 8-oxoguanine DNA glycosylase/lyase in Arabidopsis thaliana.

    abstract::Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1013644026132

    authors: García-Ortiz MV,Ariza RR,Roldán-Arjona T

    更新日期:2001-12-01 00:00:00

  • A 68 bp element of the beta-phaseolin promoter functions as a seed-specific enhancer.

    abstract::In beans, expression of the beta-phaseolin gene (phas), encoding the major seed storage protein of bean (Phaseolus vulgaris) is confined to the cotyledons of developing embryos. Phaseolin has not been detected in the endosperm, which remains liquid and is lost early in development. However, fusion constructs between t...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00020199

    authors: van der Geest AH,Hall TC

    更新日期:1996-11-01 00:00:00

  • Genome-wide transcriptomic analysis of response to low temperature reveals candidate genes determining divergent cold-sensitivity of maize inbred lines.

    abstract::Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-014-0187-8

    authors: Sobkowiak A,Jończyk M,Jarochowska E,Biecek P,Trzcinska-Danielewicz J,Leipner J,Fronk J,Sowiński P

    更新日期:2014-06-01 00:00:00

  • The promoter of a Brassica napus lipid transfer protein gene is active in a range of tissues and stimulated by light and viral infection in transgenic Arabidopsis.

    abstract::cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1006232700835

    authors: Sohal AK,Pallas JA,Jenkins GI

    更新日期:1999-09-01 00:00:00

  • Phylogenetic origin of a secondary pathway: the case of pyrrolizidine alkaloids.

    abstract::Recent studies have revealed high sequence similarity between homospermidine synthase (HSS), the first pathway-specific enzyme in the biosynthesis of pyrrolizidine alkaloids, a class of sporadically occurring plant defence compounds, and deoxyhypusine synthase (DHS), a ubiquitous enzyme involved in the post-translatio...

    journal_title:Plant molecular biology

    pub_type: 杂志文章,评审

    doi:10.1023/a:1026597621646

    authors: Ober D,Hartmann T

    更新日期:2000-11-01 00:00:00

  • Genomic analysis of the 12-oxo-phytodienoic acid reductase gene family of Zea mays.

    abstract::The 12-oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds adjacent to an oxo group in alpha,beta-unsaturated aldehydes or ketones. Some of them have very high substrate specificity and are part of the octadecanoid pathway which convert linolenic acid to the phytohormone jas...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-005-8883-z

    authors: Zhang J,Simmons C,Yalpani N,Crane V,Wilkinson H,Kolomiets M

    更新日期:2005-09-01 00:00:00

  • The reconstruction and biochemical characterization of ancestral genes furnish insights into the evolution of terpene synthase function in the Poaceae.

    abstract:KEY MESSAGE:Distinct catalytic features of the Poaceae TPS-a subfamily arose early in grass evolution and the reactions catalyzed have become more complex with time. The structural diversity of terpenes found in nature is mainly determined by terpene synthases (TPS). TPS enzymes accept ubiquitous prenyl diphosphates as...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-020-01037-4

    authors: Luck K,Chen X,Norris AM,Chen F,Gershenzon J,Köllner TG

    更新日期:2020-09-01 00:00:00

  • Phytochrome regulation of mRNA levels of ribulose-1,5-bisphosphate carboxylase in etiolated rye seedlings (Secale cereale).

    abstract::The effect of red light and far-red light on the appearance of mRNA for the small and large subunit of ribulose-1,5-bisphosphate carboxylase in rye seedlings has been analysed. Irradiation of etiolated seedlings with a pulse of red light increased the mRNA level of the small subunit by a factor of 7-10 and that of the...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00014183

    authors: Ernst D,Pfeiffer F,Schefbeck K,Weyrauch C,Oesterhelt D

    更新日期:1987-01-01 00:00:00

  • Isolation of an Arabidopsis homologue of the maize homeobox Knotted-1 gene.

    abstract::The shoot apical meristem (SAM) is responsible for forming most of the above-ground portion of the plant. We sought to isolate regulatory genes expressed in the Arabidopsis SAM by screening a Brassica oleracea (cauliflower) meristem cDNA library with the homeobox fragment from the maize Knotted-1 (Kn1) gene. We isolat...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00021797

    authors: Granger CL,Callos JD,Medford JI

    更新日期:1996-05-01 00:00:00

  • Functional analysis of the promoter region of a maize (Zea mays L.) H3 histone gene in transgenic Arabidopsis thaliana.

    abstract::A 1023 bp fragment and truncated derivatives of the maize (Zea mays L.) histone H3C4 gene promoter were fused to the beta-glucuronidase (GUS) gene and introduced via Agrobacterium tumefaciens into the genome of Arabidopsis thaliana. GUS activity was found in various meristems of transgenic plants as for other plant hi...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1023/a:1005909703600

    authors: Atanassova R,Flénet M,Gigot C,Chaubet N

    更新日期:1998-05-01 00:00:00

  • Transcriptomic adaptations in rice suspension cells under sucrose starvation.

    abstract::Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-006-9100-4

    authors: Wang HJ,Wan AR,Hsu CM,Lee KW,Yu SM,Jauh GY

    更新日期:2007-03-01 00:00:00

  • Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes.

    abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/BF00752901

    authors: Peerbolte R,Leenhouts K,Hooykaas-van Slogteren GM,Wullems GJ,Schilperoort RA

    更新日期:1986-07-01 00:00:00

  • Methyl-coenzyme M reductase-dependent endogenous methane enhances plant tolerance against abiotic stress and alters ABA sensitivity in Arabidopsis thaliana.

    abstract:KEY MESSAGE:Our study firstly elaborated the underlying mechanism of endogenous CH4-induced abiotic tolerance, along with an alteration of ABA sensitivity by mimicking the endogenous CH4 production in MtMCR transgenic Arabidopsis. Endogenous methane (CH4) production and/or emission have been ubiquitously observed in st...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-019-00914-x

    authors: Su J,Yang X,He J,Zhang Y,Duan X,Wang R,Shen W

    更新日期:2019-11-01 00:00:00

  • Identification and characterization of a hypoallergenic ortholog of Ara h 2.01.

    abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...

    journal_title:Plant molecular biology

    pub_type: 杂志文章

    doi:10.1007/s11103-008-9428-z

    authors: Ramos ML,Huntley JJ,Maleki SJ,Ozias-Akins P

    更新日期:2009-02-01 00:00:00