Abstract：
:Plant infections with cassava latent virus (CLV) were mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric copies of the virus genome. The CLV DNAs caused typical symptoms when they were inoculated in Agrobacterium strains C58, LBA4404 and a virE mutant A1026, but not other Agrobacterium strains with mutations in other vir loci or an E. coli polA strain. Virus-specific DNA forms characteristic of normal CLV infections were found after such infection. Characterization of progeny CLV DNA from selected plants identified several infectious mutants. These were found to be small insertions and/or deletions in the coat protein gene of DNA 1 and in the intergenic region of DNA 2.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Morris BA,Richardson KA,Andersen MT,Gardner RCdoi
10.1007/BF00019520subject
Has Abstractpub_date
1988-11-01 00:00:00pages
795-803issue
6eissn
0167-4412issn
1573-5028journal_volume
11pub_type
杂志文章abstract：:The Arabidopsis genome contains 10 genes belonging to the HD-Zip II family including ATHB2 and HAT2. Previous work has shown that ATHB2 is rapidly and strongly induced by light quality changes that provoke the shade avoidance response whereas HAT2 expression responds to auxin. Here, we present a genome-wide analysis o...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-008-9383-8
更新日期：2008-11-01 00:00:00
abstract：:The auxin-binding protein isolated from maize coleoptiles and characterized in detail describes an auxin recognition protein at the outer surface of the plasmalemma which mediates the auxin effect on cell elongation in maize coleoptiles. Its homologue in tobacco mesophyll protoplasts mediates the auxin effect on secre...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00019401
更新日期：1990-06-01 00:00:00
abstract：:Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027369
更新日期：1993-08-01 00:00:00
abstract：:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cyt...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0459-6
更新日期：2016-06-01 00:00:00
abstract：:MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned fr...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0156-7
更新日期：2014-03-01 00:00:00
abstract：:In this report, we describe the efficient expression and glycosylation, in insect cells, of β-phaseolin polypeptides (M r 45 and 48 kDa) from Phaseolus vulgaris, by means of a baculovirus expression vector. N-terminal sequence analysis demonstrated that the signal peptide was efficiently processed. Tunicamycin treatme...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00033603
更新日期：1988-11-01 00:00:00
abstract：:A cDNA clone was isolated by differential hybridization from a library prepared from barley leaves inoculated with the fungus Blumeria graminis f.sp. hordei (Bgh). The open reading frame of the insert (designated HvCHS2) encoded a polypeptide with 72-79% identity to chalcone synthases (CHS) and 65-68% identity to stil...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006031822141
更新日期：1998-07-01 00:00:00
abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014948
更新日期：1988-09-01 00:00:00
abstract：:The complete nucleotide sequence of the rDNA large spacer region was determined for two related legume plants, Vicia faba and Pisum sativum. In the large spacers of both plants, there were one or several tandem arrays of repeated sequences which account well for the length heterogeneity of the rDNA repeating units. Wh...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019395
更新日期：1990-06-01 00:00:00
abstract：:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the exp...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019157
更新日期：1990-09-01 00:00:00
abstract：:The nucleotide sequence for the Photosystem II gene psbC has been determined for the cyanobacterium Synechocystis 6803. The gene overlaps the last 50 bases of the psbD gene, and both genes are transcribed in the same direction, but read in different frames. This arrangement is identical to that found in all chloroplas...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00029879
更新日期：1988-07-01 00:00:00
abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1024822312038
更新日期：2003-06-01 00:00:00
abstract：:We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005708611020
更新日期：1997-04-01 00:00:00
abstract：:A chimeric gene consisting of the 5' flanking sequences of a rice glutelin gene (Gt3) linked to the chloramphenicol acetyltransferase (CAT) coding segment was introduced into tobacco via Agrobacterium tumefaciens-mediated transformation. CAT enzyme activity could be detected in extracts from seeds as early as 8 days a...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015653
更新日期：1990-01-01 00:00:00
abstract：:The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040578
更新日期：1994-01-01 00:00:00
abstract：:DNA microsatellites are ubiquitously present in eukaryotic genomes and represent a vast source of highly informative markers. We describe in this article a (GGC)n microsatellite which is widely distributed in eukaryotic genomes. Using polymerase chain reaction (PCR) techniques and DNA sequencing, we demonstrated for t...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014544
更新日期：1993-02-01 00:00:00
abstract：:In many temperate woody species, dormancy is induced by short photoperiods. Earlier studies have shown that the photoreceptor phytochrome A (phyA) promotes growth. Specifically, Populus plants that over-express the oat PHYA gene (oatPHYAox) show daylength-independent growth and do not become dormant. However, we show ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-010-9619-2
更新日期：2010-05-01 00:00:00
abstract：:Chloroplast and cytosolic triosephosphate isomerases from spinach were separated and purified to homogeneity. Both enzymes were partially sequenced by Edman degradation. Using degenerate primers designed against the amino acid sequences, a homologous probe for the chloroplast enzyme was amplified and used to isolate s...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019506
更新日期：1994-12-01 00:00:00
abstract：:As derived from a cDNA clone, the structure of the b-32 protein of Zea mays, a putative regulatory factor of zein expression, has a central acidic region separated by two domains covered by secondary structure motifs. In this work, three b-32 genomic clones were selected from two genomic libraries obtained from the ma...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019399
更新日期：1990-06-01 00:00:00
abstract：:Antisera against the N-terminal and C-terminal parts of the potential ORF IV product were used to analyse extracts from CaMV-infected turnip leaves by immunoblotting. Polypeptides of 87, 83, 82, 60 and 57 kDa were detected. The origin of these proteins is discussed. ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00027384
更新日期：1988-05-01 00:00:00
abstract：KEY MESSAGE:The design and use of existing VIGS vectors for revealing monocot gene functions are described and potential new vectors are discussed, which may expand their repertoire. Virus induced gene silencing (VIGS) is a method of transient gene silencing in plants, triggered by the use of modified viral vectors. VI...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/s11103-019-00854-6
更新日期：2019-05-01 00:00:00
abstract：KEY MESSAGE:This study provides the first genetic evidence for the role of PP2A in tuberization, demonstrating that the catalytic subunit StPP2Ac2b positively modulates tuber induction, and that its function is related to the regulation of gibberellic acid metabolism. The results contribute to a better understanding of...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-016-0555-7
更新日期：2017-02-01 00:00:00
abstract：:An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. nap...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040577
更新日期：1994-01-01 00:00:00
abstract：:In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1010620227913
更新日期：2001-05-01 00:00:00
abstract：:Plastidic acetyl-CoA carboxylase (ACCase) regulates the rate of fatty acid synthesis. This enzyme is composed of biotin carboxyl carrier protein (BCCP), biotin carboxylase (BC), and carboxyltransferase (CT), which consists of α and β subunits. Among these components, CTβ is encoded by the plastidic genome. In Arabidop...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-013-0075-7
更新日期：2013-07-01 00:00:00
abstract：:The constitutive cytosolic expression of a yeast ( Saccharomyces cerevisiae ) invertase within potato ( Solanum tuberosum ) tubers has previously been documented to produce a dramatic metabolic phenotype in which glycolysis, respiration and amino acid synthesis are markedly enhanced at the cost of starch synthesis. Th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-2525-8
更新日期：2004-09-01 00:00:00
abstract：:A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006282507095
更新日期：1999-07-01 00:00:00
abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042240
更新日期：1996-06-01 00:00:00
abstract：:We describe the isolation and characterization of maize cDNAs that are transcribed from a small gene family and encode a novel group of receptor-like kinases (RLKs). The distinctive extracellular domain of these novel RLKs includes a unique number and arrangement of leucine-rich repeats (LRRs), a proline-rich region (...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006012530241
更新日期：1998-07-01 00:00:00
abstract：:Transcription factors play an important role in regulating gene expression in response to stress and pathogen tolerance. We describe here that overexpression of an ERF/AP2 pepper transcription factor (CaPF1) in transgenic Virginia pine (Pinus virginiana Mill.) confers tolerance to heavy metals Cadmium, Copper, and Zin...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0451-z
更新日期：2005-11-01 00:00:00
