Abstract:
:By differential screening of a cDNA library of two-week-old rice seedlings cDNA clones were obtained, corresponding to shoot-specific mRNAs. By sequence analysis two of these clones were found to be rbcS cDNA clones. The mRNA corresponding to a third cDNA clone (COS5) displayed an expression pattern similar to the expression pattern of rbcS genes. The mRNA (800 bases) was light-inducible and encoded by a single-copy gene. The genomic clone (GOS5) was isolated and the intron/exon structure was determined by comparing the nucleotide sequences of the mRNA and the genomic clone. The gene contains two introns. Transcription start sites were determined by S1-nuclease mapping and primer extension. The start site obtained by both methods is located 87 bp upstream of the translation start site and 23 bp downstream of TATA box-like sequence. In the 5' non-coding region motifs can be found that are homologous to sequences in promoters that are light- or UV-inducible or confer leaf-specific expression. The open reading frame present in GOS5 codes for a protein (15 kDa) that contains a putative chloroplast transit peptide and does not show any significant homology to protein sequences in the NBRF protein database.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
de Pater S,Hensgens LA,Schilperoort RAdoi
10.1007/BF00019157subject
Has Abstractpub_date
1990-09-01 00:00:00pages
399-406issue
3eissn
0167-4412issn
1573-5028journal_volume
15pub_type
杂志文章abstract::Catalase 2 (CAT2) plays an important role in the detoxification of hydrogen peroxide released either during photorespiration or as a consequence of biotic and abiotic stress as well as in the initiation of senescence. To date, our understanding of the regulation of CAT2 gene expression is rather poor. Chromatin immuno...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0546-8
更新日期:2017-01-01 00:00:00
abstract::Transfer RNA genes have been mapped to at least nine different loci on the physical map of the Euglena gracilis chloroplast genome. One of these loci in the ribosomal RNA operons is present three times per genome. The DNA sequences of six of the nine different loci, containing 21 different tRNA genes, have been determ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016064
更新日期:1984-05-01 00:00:00
abstract::Histone acetylation is modulated through the action of histone acetyltransferase and deacetylase, which play key roles in the regulation of eukaryotic gene expression. We have screened the expressed sequence tag database with the yeast histone deacetylase RPD3 sequence and identified two Arabidopsis homologues, AtRPD3...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006498413543
更新日期:2000-09-01 00:00:00
abstract::Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9866-x
更新日期:2012-02-01 00:00:00
abstract::Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response to cold was studied in two inbred lines of contrasting cold-tolerance. Microarray analysis was followed by extensive ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0187-8
更新日期:2014-06-01 00:00:00
abstract::GALACTINOL SYNTHASE is the first committed enzyme in the raffinose biosynthetic pathway. We have previously characterized the maize (Zea mays) GALACTINOL SYNTHASE2 gene (ZmGOLS2) as abiotic stress induced. To further investigate the regulation of ZmGOLS2 gene expression, individual luciferase expression vectors,in whi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0403-1
更新日期:2016-01-01 00:00:00
abstract::Green cybrids with a new nucleus-chloroplast combination cannot be selected after protoplast fusion in the intersubfamilial Nicotiana-Solanum combination. As an approach to overcome the supposed plastomegenome incompatibility, a partial plastome transfer by genetic recombination has been considered. After fusions of p...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017450
更新日期:1989-01-01 00:00:00
abstract::The soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. Five highly conserved regions were found. There are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enha...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027380
更新日期:1993-08-01 00:00:00
abstract::We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027495
更新日期:1990-04-01 00:00:00
abstract::The genetics of male fertility restoration and the RFLP of mitochondrial DNA were studied for 16 sunflower cytoplasms (15 male-sterile and a male-fertile). Male fertility restoration/male sterility maintenance patterns distinguished 12 cytotypes. Four cytoplasms were completely unrestored so they were not distinguishe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023992
更新日期:1991-03-01 00:00:00
abstract::We have cloned, sequenced, and examined the expression of genes from pine trees that appear to encode extracellular class II chitinase. Nucleotide sequence analysis indicates a coding sequence composed of three exons interrupted by two introns at locations identical to those found in other chitinase genes that possess...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005708611020
更新日期:1997-04-01 00:00:00
abstract::Two dimensional gel electrophoresis was used to examine differences in steady state total poly(A) RNA from untransformed potato (Solanum tuberosum cv. Maris Bard) and potato transformed with shoot-inducing TL-DNA from A. tumefaciens. RNA was compared from phenotypically very distinct in vitro cultured shoots, more sim...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00015227
更新日期:1986-07-01 00:00:00
abstract::We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9284-2
更新日期:2008-03-01 00:00:00
abstract::Porphobilinogen deaminase (PBG deaminase) is an early enzyme of the pathway for chlorophyll and heme synthesis. Using degenerate oligonucleotide primers, based on amino acid sequence data for purified PBG deaminase from pea, a fragment was amplified from Arabidopsis genomic DNA by PCR, and then used to isolate both a ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028854
更新日期:1994-11-01 00:00:00
abstract::TPD (tapping panel dryness) is a complex physiological syndrome widely found in rubber tree (Hevea brasiliensis) plantations, which causes severe yield and crop losses in natural rubber-producing countries. The molecular mechanism underlying TPD is not known and there is presently no effective prevention or treatment ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1020719420867
更新日期:2003-01-01 00:00:00
abstract::Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts e...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-3688-7
更新日期:2006-01-01 00:00:00
abstract::The chrysanthemum is one of popular flowers in the world and a host for several viruses. So far, molecular interaction studies between the chrysanthemum and viruses are limited. In this study, we carried out a transcriptome analysis of chrysanthemum in response to three different viruses including Cucumber mosaic viru...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-015-0317-y
更新日期:2015-06-01 00:00:00
abstract::In this study a size selected genomic library from Quercus petraea was screened for (GA/CT)n-microsatellite sequences. The resulting loci were analysed by PCR for their usefulness as molecular markers in Q. petraea and Q. robur. 17 out of 52 tested primer pairs resulted in the amplification of a polymorphic single-loc...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005736722794
更新日期:1997-04-01 00:00:00
abstract::In plants, cytosolic Ca2+ levels are tightly regulated, and changes in cytosolic Ca2+ have been implicated in converting numerous signals into adapted responses. Vacuolar ion transporters are thought to be key mediators of cytosolic Ca2+ concentrations. In an attempt to interpret the role of vacuolar Ca2+ transport in...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010620227913
更新日期:2001-05-01 00:00:00
abstract::Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00042230
更新日期:1996-06-01 00:00:00
abstract::We describe a non-LTR retrotransposon family,BvL, of the long interspersed nuclear elements L1 clade isolated from sugar beet (Beta vulgaris). Characteristic molecular domains of three full-length BvL elements were determined in detail, showing that coding sequences are interrupted and most likely non-functionally. In...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9542-6
更新日期:2009-12-01 00:00:00
abstract::Controlled cellular suicide is an important process that can be observed in various organs during plant development. From the generation of proper sexual organs in monoecious plants to the hypersensitive response (HR) that occurs during incompatible pathogen interactions, programmed cell death (PCD) can be readily obs...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006133311402
更新日期:1999-04-01 00:00:00
abstract::Polyphenol oxidases (PPOs) catalyze the oxidation of ortho-diphenols to the corresponding quinones (EC 1.10.3.1). In plants PPOs appear in gene families, and the corresponding isoenzymes are located to the thylakoid lumen of chloroplasts. Although plant PPOs are often discussed with regard to their role in defense rea...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9943-9
更新日期:2012-09-01 00:00:00
abstract::Cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. The gene products participate either directly or indirectly towards increasing cold tolerance. Evidence of proteins having a direct effect on cold tolerance is emerging but limited. With isolated protoplasts from warm...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1026599028063
更新日期:2000-11-01 00:00:00
abstract::A partial cDNA clone (PSnir) encoding the C-terminal region of nitrite reductase was isolated from a lambda gt11 library of the gymnosperm Pimus sylvestris (L.). Nucleotide sequence analysis showed that PSnir contains a reading frame encoding 105 amino acid residues. The amino acid sequence revealed a homology to NiR ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043873
更新日期:1994-06-01 00:00:00
abstract::ATP-binding cassette transporters are involved in the active transport of a wide variety of metabolites in prokaryotes and eukaryotes. One subfamily, the Pleiotropic Drug Resistance (PDR) transporters, or full-size ABCG transporters, are found only in fungi and plants. NtPDR1 was originally identified in Nicotiana tab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-013-0053-0
更新日期:2013-05-01 00:00:00
abstract::Strigolactones have recently been identified as the long sought-after signal required to inhibit shoot branching (Gomez-Roldan et al. 2008; Umehara et al. 2008; reviewed in Dun et al. 2009). Here we briefly describe the evidence for strigolactone inhibition of shoot branching and, more extensively, the broader context...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-010-9599-2
更新日期:2010-05-01 00:00:00
abstract::The nucleotide sequence of a cDNA prepared from poly(A)+ RNA from Lycopersicon esculentum fruit codes for a protein, M(r) 20,812, with features representative of the protein core of arabinogalactan proteins. The deduced amino acid sequence resembles that of peptides of arabinogalactan proteins isolated from carrot and...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020254
更新日期:1995-05-01 00:00:00
abstract:KEY MESSAGE:We reported the functional characterization of cDNAs encoding short-chain isoprenyl diphosphate synthases that control the partitioning of precursors for lavender terpenoids. Lavender essential oil is composed of regular and irregular monoterpenes, which are derived from linear precursors geranyl diphosphat...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-020-00962-8
更新日期:2020-03-01 00:00:00
abstract::Heat shock proteins (hsps) are synthesized at higher temperatures in soybean seedlings exposed to a gradual temperature increase as compared to a rapid heat shock (hs). There are differences in the pattern of synthesis of different hsps depending on whether the temperature exposure is rapid or gradual. The synthesis o...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020626
更新日期:1985-09-01 00:00:00