Abstract:
:Mitochondrial maturation during imbibition of rice embryos follows the transition of unstructured double membrane bound pro-mitochondria to the typical cristae-rich mitochondrial structures observed in mature plant cells. During the first 48 h following imbibition, an ordered increase in the abundance of transcripts encoding mitochondrial proteins was observed. Co-incident with these changes in transcript levels was dynamic and rapid changes in mitochondrial protein content and mitochondrial function. Proteins representing components of the mitochondrial protein import apparatus are strikingly abundant in dry seeds, and a functional import apparatus was shown to operate 2 h after imbibition. Interestingly, this import process was best driven by the oxidation of NADH from outside the mitochondrial inner membrane. In later developmental stages the capacity for matrix organic acid metabolism was evident, accompanied by the appearance of proteins for TCA cycle components, and coordination of electron transport chain assembly through components encoded in both mitochondrial and nuclear genomes. Together these events provide new insights into the understanding of mitochondrial maturation and the nature of pro-mitochondrial structures in plant cells.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Howell KA,Millar AH,Whelan Jdoi
10.1007/s11103-005-3688-7subject
Has Abstractpub_date
2006-01-01 00:00:00pages
201-23issue
2eissn
0167-4412issn
1573-5028journal_volume
60pub_type
杂志文章abstract::Arabidopsis is currently the reference genome for higher plants. A new, more detailed statistical analysis of Arabidopsis gene structure is presented including intron and exon lengths, intergenic distances, features of promoters, and variant 5'-ends of mRNAs transcribed from the same transcription unit. We also provid...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-005-2564-9
更新日期:2006-01-01 00:00:00
abstract::The LfKCS45 gene with a high sequence similarity to known 3-ketoacyl-CoA synthases of the membrane-bound fatty acid elongase was isolated from Lesquerella fendleri. The LfKCS45 gene has a 1464 bp open reading frame without introns, and is predicted to encode a polypeptide of 487 amino acids with an estimated molecular...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-004-6235-z
更新日期:2004-12-01 00:00:00
abstract::Tomato fruit maturation is accompanied by a depolymerization of cell wall pectins which is due to the action of endopolygalacturonase (endoPG) preceded by pectin methylesterase (PE) activity. To investigate the role of endoPG and PE in determining the structure of green bean (Phaseolus vulgaris L.) pectins, these pect...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040831
更新日期:1996-09-01 00:00:00
abstract::Primer extension was used to characterize alpha-amylase mRNAs from aleurone tissue of barley (Hordeum vulgare L. cv. Himalaya) grains. Two synthetic oligonucleotides, specific for the low-pI and high-pI alpha-amylase groups, were used as primers for synthesis of cDNA from total RNA preparations. Between them, these tw...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00023427
更新日期:1991-04-01 00:00:00
abstract::Egy1 was isolated as an ethylene-dependent gravitropism-deficient Arabidopsis mutant. Molecular studies reveal that EGY1 gene encodes a 59-kDa plastid-targeted metalloprotease. It is actively expressed in hypocotyl tissue and targets to endodermal and cortex plastid. Its protein level is up-regulated by both ethylene ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9273-5
更新日期:2008-03-01 00:00:00
abstract::cDNA clones for all enzymes of the prechorismate pathway of higher plants have previously been cloned, with the exception of the second enzyme of the pathway, 3-dehydroquinate synthase. Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020607
更新日期:1996-04-01 00:00:00
abstract::We have cloned and sequenced a full-length cDNA for uroporphyrinogen decarboxylase (UROD, EC 4.1.1.37) from tobacco (Nicotiana tabacum L.) and a partial cDNA clone from barley (Hordeum vulgare L.). The cDNA of tobacco encodes a protein of 43 kDa, which has 33% overall similarity to UROD sequences determined from other...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020244
更新日期:1995-05-01 00:00:00
abstract::The nuclear gene mutant of barley, vir-115, shows a developmentally induced loss of D1 synthesis that results in inactivation of Photosystem II. Translation in plastids isolated from 1 h illuminated vir-115 seedlings is similar to wild type. In wild-type barley, illumination of plants for 16 to 72 h results in increas...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00043874
更新日期:1994-06-01 00:00:00
abstract:KEY MESSAGE:MdMYB16 forms homodimers and directly inhibits anthocyanin synthesis via its C-terminal EAR repressor. It weakened the inhibitory effect of MdMYB16 on anthocyanin synthesis when overexpressing MdbHLH33 in callus overexpressing MdMYB16. MdMYB16 could interact with MdbHLH33. Anthocyanins are strong antioxidan...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-017-0601-0
更新日期:2017-05-01 00:00:00
abstract::The sequence of the S-adenosyl-L-methionine:trans-caffeoyl-CoA O-methyltransferase (CCoAOMT, EC2.1.1.104) gene, including the 5'-flanking region of 5 kb, was determined from parsley (Petroselinum crispum) plants. The enzyme appears to be encoded by one or two genes, and the ORF is arranged in five exons spaced by intr...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005780529457
更新日期:1997-01-01 00:00:00
abstract::As part of a strategy to determine the precise role of pea (Pisum sativum) lectin, Psl, in nodulation of pea by Rhizobium leguminosarum, mutations were introduced into the genetic determinant for pea lectin by site-directed mutagenesis using PCR. Introduction of a specific mutation, N125D, into a central area of the s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028892
更新日期:1992-12-01 00:00:00
abstract::A leaf undergoes determinate growth from a primordium on flank of the shoot apical meristem. Several intrinsic pathways restrict meristematic activity in the leaf of Arabidopsis; however, other factors remain to be defined. We report here that the overexpression of MINI ZINC FINGER1 (MIF1) or MIF3 disrupted the leaf d...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9768-y
更新日期:2011-05-01 00:00:00
abstract::To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones wer...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9780-2
更新日期:2011-05-01 00:00:00
abstract::Tomato golden mosaic virus (TGMV), a member of the geminivirus group, has a genome consisting of two DNA molecules designated the A and B components. Both are required for infectivity in healthy plants, although the former has been shown to replicate independently in transgenic plants containing tandem direct repeats ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00017993
更新日期:1987-11-01 00:00:00
abstract::The cis-regulatory elements that confer cell cycle-dependent expression to the wheat histone H3 gene were investigated in rice cells (Oc strain) transformed with H3/GUS chimeric genes. 5' deletion mutants of the H3 promoter region (from -1711, -908 or -185 to +57 relative to the transcription start site) were joined t...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00019303
更新日期:1993-11-01 00:00:00
abstract::Pleiotropic effects are one of the main concerns regarding genetically modified organisms (GMOs). This includes unintended side effects of the transgene or its genome insertion site on the regulation of other endogenous genes, which could potentially cause the accumulation of different secondary metabolites that may h...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-007-9229-9
更新日期:2007-11-01 00:00:00
abstract:KEY MESSAGE:We identified and cloned the two precursors of miR158 and its target gene in Brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. Further study revealed that over-expression of miR158 caused reduced pollen varbility, which was caused by the degradation of pollen...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0563-7
更新日期:2017-02-01 00:00:00
abstract::The soluble acyl-ACP:sn-glycerol-3-phosphate acyltransferase from chloroplasts of chilling-sensitive and -resistant plants differ in their fatty acid selectivity. Enzymes from resistant plants discriminate against non-fluid palmitic acid and select oleic acid whereas the acyltransferase from sensitive plants accepts b...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037145
更新日期:1991-11-01 00:00:00
abstract::A leaf cDNA library from hybrid aspen, Populus tremula x tremuloides, was constructed. From this two different cDNA clones, denoted CA1a and CA1b, encoding a chloroplastic carbonic anhydrase (CA) were isolated and DNA sequenced. Analysis of the deduced amino acid sequences showed that the isolated CAs belong to the be...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005849202731
更新日期:1997-07-01 00:00:00
abstract::Expression of the chloroplast electron shuttle ferredoxin is induced by light through mechanisms that partially depend on sequences lying in the coding region of the gene, complicating its manipulation by promoter engineering. Ferredoxin expression is also down-regulated under virtually all stress situations, and it i...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9786-9
更新日期:2011-08-01 00:00:00
abstract::The cell cycle of eukaryotes is tightly regulated through the activity of cyclin-dependent kinases. The Arabidopsis thaliana CDKA;1 (CDC2aAt) gene is thought to encode such a protein kinase, since it is actively transcribed in proliferating tissues and can complement defects in the Schizosaccharomyces pombe cdc2 gene....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010665831955
更新日期:2001-05-01 00:00:00
abstract::The nucleotide sequence distal to the rRNA operon from maize chloroplasts has been analyzed. It contains genes coding for tRNA(Arg)(ACG) and tRNA(Asn)(GUU). The tRNA(Arg)(ACG) gene, which is separated from the last rRNA gene of the rRNA operon, the 5S rDNA, by an intergenic region of 252 bp, has the same orientation a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020326
更新日期:1986-11-01 00:00:00
abstract::Transgenic Arabidopsis thaliana plants expressing cel1 antisense exhibit reduced levels of cel1 mRNA and protein compared with wild-type plants. The former display significant alterations in their phenotype. cel1 antisense plants have shorter stems and roots and are mechanically weaker than their wild-type counterpart...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1021162321527
更新日期:2003-01-01 00:00:00
abstract::Several clones corresponding to the gene encoding NADH-dependent hydroxypyruvate reductase have been isolated from a cucumber genomic library. Restriction mapping indicates the presence of two HPR genes, hpr-A and hpr-B, in the cucumber genome. Examination of the DNAs of individual plants suggests that hpr-A and hpr-B...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037078
更新日期:1991-10-01 00:00:00
abstract::We have previously shown that mRNA and protein encoded by the Pvlea-18 gene from Phaseolus vulgaris L., a member of a new family of late embryogenesis-abundant (LEA) proteins, accumulate in dark-grown bean seedlings not only in response to water deficit but also during optimal irrigation. In this work, we studied Pvle...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1010607223533
更新日期:2001-03-01 00:00:00
abstract::Plastids possess a bacteria-like sec apparatus that is involved in protein import into the thylakoid lumen. We have analyzed one of the genes essential for this process, secY. A secY gene from the unicellular red alga Cyanidium caldarium was found to be transcriptionally active, demonstrating for the first time that s...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020209
更新日期:1996-11-01 00:00:00
abstract::In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016071
更新日期:1991-06-01 00:00:00
abstract::The relative amounts of some chloroplast polypeptides were followed during greening of leaves from three different plant families. Oat, bean and spinach were the representatives of the Gramineae, Leguminosae and Chenopodiaceae, respectively. By using specific antibodies against subunits of the chloroplast protein comp...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF02418259
更新日期:1985-11-01 00:00:00
abstract::Heat shock protein 90 (Hsp90) is an abundant and highly conserved molecular chaperone. In Arabidopsis, the Hsp90 gene family consists of seven members. Here, we report that the AtHsp90-6 gene gives rise to two mRNA populations, termed AtHsp90-6L and AtHsp90-6S due to alternative initiation of transcription. The AtHsp9...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9322-8
更新日期:2008-07-01 00:00:00
abstract::Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genet...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:
更新日期:1997-09-01 00:00:00