Abstract:
:Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3' non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3' non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our success can be attributed to shorter 3' non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Joshi CP,Nguyen HTdoi
10.1007/BF00042230subject
Has Abstractpub_date
1996-06-01 00:00:00pages
575-84issue
3eissn
0167-4412issn
1573-5028journal_volume
31pub_type
杂志文章abstract::Sugar is an important resource for energy generation and developmental regulation in plants, and sucrose starvation causes enormous changes in cellular morphology, enzyme activities and gene expression. Genome-wide gene expression profiling provides a comprehensive knowledge of gene expression under nutrient depletion...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9100-4
更新日期:2007-03-01 00:00:00
abstract::Plant cell suspension cultures are invaluable models for the study of cellular processes. Here we develop the recently described Arabidopsis suspension culture MM2d as a transcript profiling platform by means of Affymetrix ATH1 microarrays. Analysis of gene expression profiles during normal culture growth, during sync...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000019059.56489.ca
更新日期:2003-11-01 00:00:00
abstract::Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-014-0195-8
更新日期:2014-07-01 00:00:00
abstract::The chloroplast gene for the epsilon subunit (atpE) of the CF1/CF0 ATPase in the green alga Chlamydomonas reinhardtii has been localized and sequenced. In contrast to higher plants, the atpE gene does not lie at the 3' end of the beta subunit (atpB) gene in the chloroplast genome of C. reinhardtii, but is located at a...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00025326
更新日期:1987-03-01 00:00:00
abstract::Three members have been isolated of an additional glutelin gene subfamily, named subfamily B, consisting of about five members per haploid rice genome. Restriction fragment length polymorphism analysis showed major differences between Japonica and Indica lines, indicating the divergence of the subfamily since the spli...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00037068
更新日期:1991-10-01 00:00:00
abstract::Miniature inverted-repeat transposable elements (MITEs) are short, non autonomous DNA elements that are widespread and abundant in plant genomes. The high sequence and size conservation observed in many MITE families suggest that they have spread recently throughout their respective host genomes. Here we present a mai...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-009-9511-0
更新日期:2009-09-01 00:00:00
abstract::A cDNA subtraction and differential hybridization strategy was used to isolate cDNAs expressed early during male gametophyte development in the important crop species Brassica napus. Three cDNAs, corresponding to genes highly and specifically expressed at the tetrad and microspore stages, are presented here. The analy...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006282507095
更新日期:1999-07-01 00:00:00
abstract::cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino ac...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00040617
更新日期:1992-11-01 00:00:00
abstract::We have cloned and sequenced a gene, pds, from the cyanobacterium Synechococcus PCC7942 that is responsible for resistance to the bleaching herbicide norflurazon. A point mutation in that gene, leading to an amino acid substitution from valine to glycine in its polypeptide product, was found to confer this resistance....
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00016069
更新日期:1991-06-01 00:00:00
abstract::The plant senescence syndrome resembles, in many molecular and phenotypic aspects, plant responses to abiotic stresses. Both processes have an enormous negative global agro-economic impact and endanger food security worldwide. Premature plant senescence is the main cause of losses in grain filling and biomass yield du...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-013-0038-z
更新日期:2013-08-01 00:00:00
abstract::Tight control of cell/tissue identity is essential for a correct and functional organ patterning, an important component of overall fruit development and eventual maturation and ripening. Despite many investigations regarding the molecular determinants of cell identity in fruits of different species, a useful model ab...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-016-0445-z
更新日期:2016-05-01 00:00:00
abstract::This is the first report to describe the detailed analysis of TATA sequence requirements for the initiation of transcription for RNA polymerase II in a plant in vitro transcription system. We examined the transcriptional activities associated with 24 promoters, including wild-type (TATATATA), and the 21 point and two ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006056128129
更新日期:1998-12-01 00:00:00
abstract::Transformed clones from a shooty tobacco crown gall tumor, induced byAgrobacterium tumefaciens strain LBA1501, having the auxin locus of the TL-region inactivated by a Tn1831 insertion, were investigated for their T-DNA structure and expression. It has been described previously (28) that in addition to clones with an ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00752901
更新日期:1986-07-01 00:00:00
abstract:KEY MESSAGE:Plant bioregulators play an important role in managing oxidative stress tolerance in plants. Utilizing their ability in stress sensitive crops through genetic engineering will be a meaningful approach to manage food production under the threat of climate change. Exploitation of the plant defense system agai...
journal_title:Plant molecular biology
pub_type: 杂志文章,评审
doi:10.1007/s11103-020-01077-w
更新日期:2021-01-01 00:00:00
abstract::The colour of the red wine is essentially due to the release of anthocyanins from the red skin of grape berries during the process of wine making. Anthocyanins are synthesized during ripening of the berries under the control of VvMYBA1 transcription factor that controls the expression of UFGT. In order to identify the...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9446-x
更新日期:2009-04-01 00:00:00
abstract::We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tis...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027495
更新日期:1990-04-01 00:00:00
abstract::Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the most potent peanut allergen and is recognize...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-008-9428-z
更新日期:2009-02-01 00:00:00
abstract:KEY MESSAGE:A Kelch repeat F-box containing protein, SMALL AND GLOSSY LEAVES1 (SAGL1) regulates phenylpropanoid biosynthesis as a post-translational regulator for PAL1 (phenylalanine ammonia-lyase) and an indirect transcriptional regulator for ANTHOCYANIDIN SYNTHASE. Phenylpropanoid biosynthesis in plants produces dive...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0808-8
更新日期:2019-01-01 00:00:00
abstract::We have constructed a yeast artificial chromosome (YAC) library using high-molecular-weight DNA prepared from agarose-embedded leaf protoplasts of the maize inbred line UE95. This library contains 79,000 clones with an average insert size of 145 kb and should therefore represent approximately three haploid genome equi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027351
更新日期:1992-05-01 00:00:00
abstract::Retroviruses and retrotransposons make up the broad class of retroelements replicating and transposing via reverse transcriptase. Retroelements have recently been found to be ubiquitous in the plants. We report here the isolation, sequence and analysis of a retroelement from barley (Hordeum vulgare L.) with all the fe...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00027369
更新日期:1993-08-01 00:00:00
abstract::Leaves develop as flat lateral organs from the indeterminate shoot apical meristem. The establishment of polarity along three-dimensional axes, proximal-distal, medial-lateral, and adaxial-abaxial axes, is crucial for the growth of normal leaves. The mutations of ASYMMETRIC LEAVES1 (AS1) and AS2 of Arabidopsis thalian...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-012-9929-7
更新日期:2012-08-01 00:00:00
abstract::Efficient and cost-effective conversion of plant biomass to usable forms of energy requires a thorough understanding of cell wall biosynthesis, modification and degradation. To elucidate these processes, we assessed the expression dynamics during enzymatic removal and regeneration of rice cell walls in suspension cell...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-011-9819-4
更新日期:2011-11-01 00:00:00
abstract:KEY MESSAGE:Based on the nature of the proteins that are altered in abundance, we conclude that guard cells switch their energy source from fatty acid metabolism to chloroplast activity, at the onset of dawn. During stomatal opening at dawn, evidence was recently presented for a breakdown and liquidation of stored tria...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-018-0794-x
更新日期:2018-12-01 00:00:00
abstract::The expression of tobacco class I chitinase gene is effectively induced by a fungal elicitor in suspension-cultured tobacco cells. To identify cis-acting DNA elements that respond to the elicitor, a series of promoter constructs of the chitinase gene CHN50 fused to beta-glucuronidase gene was introduced into tobacco c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00024116
更新日期:1994-02-01 00:00:00
abstract::Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1005959203396
更新日期:1997-12-01 00:00:00
abstract::We have characterized two sets of cDNA clones representing the glutamine synthetase (GS) mRNA in soybean nodules. Using the 3'-untranslated regions of a representative member of each set, as gene member(s) specific probes, we have shown that one set of the GS genes are expressed in a nodule-specific manner, while the ...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00028970
更新日期:1993-09-01 00:00:00
abstract::The maize Cat2 gene was isolated by direct cloning and PCR. The clones were mapped and sequenced. The start site of transcription was determined by primer extension. Computer analysis of the 1.6 kb Cat2 promoter sequence has revealed an obvious TATA box, tow GC boxes, a putative GA response element, and several ACGT c...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/BF00020803
更新日期:1996-03-01 00:00:00
abstract::cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/a:1006232700835
更新日期:1999-09-01 00:00:00
abstract::Glucosinolates are a class of thioglycosides found predominantly in plants of the order Brassicales whose function in anti-herbivore defense has been attributed to the products formed by myrosinase-catalyzed hydrolysis upon plant tissue damage. The most common type of hydrolysis products, the isothiocyanates, are toxi...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1007/s11103-006-9071-5
更新日期:2007-01-01 00:00:00
abstract::Coordinated cell growth and differentiation is crucial for the development of higher plants. Using the elongation defective 1-1 (eld1-1) mutant, we cloned the ELD1 gene, which encodes a serine-rich protein. Genes homologous to ELD1 can be found in plants, including Arabidopsis, rice, and tobacco, but not in other orga...
journal_title:Plant molecular biology
pub_type: 杂志文章
doi:10.1023/B:PLAN.0000019067.05185.d6
更新日期:2003-11-01 00:00:00