Abstract：
:Glycine betaine (betaine) has the highest cellular osmoprotective efficiency which does not accumulate in most glycophytes. The biosynthetic pathway for betaine in higher plants is derived from the oxidation of low-accumulating metabolite choline that limiting the ability of most plants to produce betaine. Halophilic methanoarchaeon Methanohalophilus portucalensis FDF1(T) is a model anaerobic methanogen to study the acclimation of water-deficit stresses which de novo synthesize betaine by the stepwise methylation of glycine, catalyzed by glycine sarcosine N-methyltransferase (GSMT) and sarcosine dimethylglycine N-methyltransferase. In this report, genes encoding these betaine biosynthesizing enzymes, Mpgsmt and Mpsdmt, were introduced into Arabidopsis. The homozygous Mpgsmt (G), Mpsdmt (S), and their cross, Mpgsmt and Mpsdmt (G × S) plants showed increased accumulation of betaine. Water loss from detached leaves was slower in G, S, and G × S lines than wild-type (WT). Pot-grown transgenic plants showed better growth than WT after 9 days of withholding water or irrigating with 300 mM NaCl. G, S, G × S lines also maintained higher relative water content and photosystem II activity than WT under salt stress. This suggests heterologously expressed Mpgsmt and Mpsdmt could enhance tolerance to drought and salt stress in Arabidopsis. We also found a twofold increase in quaternary ammonium compounds in salt-stressed leaves of G lines, presumably due to the activation of GSMT activity by high salinity. This study demonstrates that introducing stress-activated enzymes is a way of avoiding the divergence of primary metabolites under normal growing conditions, while also providing protection in stressful environments.
journal_name
Plant Mol Bioljournal_title
Plant molecular biologyauthors
Lai SJ,Lai MC,Lee RJ,Chen YH,Yen HEdoi
10.1007/s11103-014-0195-8subject
Has Abstractpub_date
2014-07-01 00:00:00pages
429-41issue
4-5eissn
0167-4412issn
1573-5028journal_volume
85pub_type
杂志文章abstract：:Full-length cDNAs encoding the alpha- and beta-subunits and a truncated mutant subunit of the Chlorella sorokiniana NADP-GDH isozymes were constructed and expressed in Escherichia coli cells. The kinetic and thermal stability properties of the resultant homohexamers were examined. The electrophoretic mobility of the r...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1024822312038
更新日期：2003-06-01 00:00:00
abstract：:The hypersensitive response (HR) involves programmed cell death (PCD) in response to pathogen infection. To investigate the pathogen resistance signaling pathway, we previously identified the Arabidopsis mutant cpr22, which displays constitutive activation of multiple defense responses including HR like cell death. Th...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9239-7
更新日期：2007-12-01 00:00:00
abstract：:Repair of the ubiquitous mutagenic lesion 7,8-dihydro-8-oxoguanine (8-oxoG) is initiated in eukaryotes by DNA glycosylases/lyases, such as yeast Ogg1, that do not share significant sequence identity with their prokaryotic counterparts, typified by Escherichia coli MutM (Fpg) protein. The unexpected presence of a funct...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1013644026132
更新日期：2001-12-01 00:00:00
abstract：:The identification of plant genes involved in early phases of in vitro morphogenesis can not only contribute to our understanding of the processes underlying growth regulator-controlled determination, but also provide novel markers for evaluating the outcome of in vitro regeneration experiments. To search for such gen...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020486
更新日期：1996-12-01 00:00:00
abstract：:Two cultivars of Perilla frutescens, red and green formas are known to differ in anthocyanin accumulation in leaves and stems. cDNA clones encoding the enzymes involved in anthocyanin biosynthesis, chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), and UDP glucose: flavonoid 3-O...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1005959203396
更新日期：1997-12-01 00:00:00
abstract：:Cab-1 is a complex genetic locus in tomato consisting of four clustered genes encoding chlorophyll a/b-binding polypeptide. Southern blot analysis of total tomato DNA with genomic clones corresponding to the Cab-1 locus has revealed the presence of a repetitive element in the 3 kb spacer regions between two of these g...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00014948
更新日期：1988-09-01 00:00:00
abstract：:Ferulate 5-hydroxylase (F5H) of the monolignol pathway catalyzes the hydroxylation of coniferyl alcohol, coniferaldehyde and ferulic acid to produce 5-hydroxyconiferyl moieties, which lead to the formation of sinapic acid and syringyl (S) lignin monomers. In contrast, guaiacyl (G) lignin, the other major type of ligni...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-020-00991-3
更新日期：2020-06-01 00:00:00
abstract：:Ustilago maydis killer toxins are small polypeptides (7-14 kDa) which kill susceptible cells of closely related fungal species. The KP4 toxin is a single polypeptide subunit with a molecular weight of 11.1 kDa. In this work, a transgenic tobacco plant was constructed which secretes the KP4 toxin at a high level. The K...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00020122
更新日期：1996-01-01 00:00:00
abstract：KEY MESSAGE:CYP71Z18 exhibited plastic substrate specificity to catalyze oxidation of multiple rice diterpenes and elevated chemical defense against the blast fungus in transgenic rice. Diversified plant specialized metabolism relies on corresponding biosynthetic enzymes with differential substrate specificity. CYP71Z1...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00881-3
更新日期：2019-08-01 00:00:00
abstract：:Chimeric genes containing the coding sequence for bacterial chloramphenicol acetyl transferase (CAT) have been introduced by electroporation into maize protoplasts (Black Mexican Sweet) and transient expression monitored by enzyme assays. Levels of CAT expression were enhanced 12-fold and 20-fold respectively by the i...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00039430
更新日期：1990-12-01 00:00:00
abstract：:Salicylic acid (SA) is a stress-induced hormone involved in the activation of defense genes. Here we analyzed the early genetic responses to SA of wild type and npr1-1 mutant Arabidopsis seedlings, using Complete Arabidopsis Transcriptome MicroArray (CATMAv2) chip. We identified 217 genes rapidly induced by SA (early ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9458-1
更新日期：2009-05-01 00:00:00
abstract：:The ability to modify plant traits is of great commercial potential in agricultural biotechnology. To this end we have engineered plant-based zinc finger protein transcription factors (ZFP TFs) that minimize the use of non-plant DNA sequences. This novel architecture supports the use of tandem arrays of zinc-finger DN...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-7820-x
更新日期：2005-02-01 00:00:00
abstract：:cDNA and genomic clones encoding Brassica napus non-specific lipid transfer proteins (LTP) were isolated and sequenced. The encoded amino acid sequences were very similar to those reported previously for LTPs from B. napus and other species. Sequence information indicates that B. napus contains an LTP gene family. The...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006232700835
更新日期：1999-09-01 00:00:00
abstract：:We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submer...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-007-9284-2
更新日期：2008-03-01 00:00:00
abstract：:To study the pattern of gene regulation of the plastid chaperonin 60 beta gene family a chimaeric gene was constructed fusing the 5'-flanking region of the chaperonin 60 beta B3 gene to the beta-glucuronidase reporter gene. Histochemical and fluorometric analysis of the GUS activity present in transgenic plants harbou...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00040585
更新日期：1994-01-01 00:00:00
abstract：:Salicylic acid (SA) is known to play an important role in the interaction between plant and micro-organisms, both symbiotic and pathogen. In particular, high levels of SA block nodule formation and mycorrhizal colonization in plants. A mutant of Lotus japonicus, named Ljsym4-2, was characterized as unable to establish...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-009-9585-8
更新日期：2010-03-01 00:00:00
abstract：:The LI818 gene from Chlamydomonas encodes a polypeptide that is related to the chlorophyll a/b-binding proteins (CAB) of higher plants and green algae. However, despite this relatedness, LI818 gene expression is not coordinated with that of cab genes and is regulated differently by light, suggesting a different role f...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006340308077
更新日期：2000-01-01 00:00:00
abstract：:Plants are capable to de novo synthesize the essential amino acids leucine, isoleucine and valine. Studies in recent years, however, also revealed that plants have the potential to degrade leucine or may be all of the branched-chain amino acids. One of the enzymes participating in both biosynthesis and degradation is ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-004-7533-1
更新日期：2005-01-01 00:00:00
abstract：:Several signal transduction pathways play important roles in the sexual life cycle of Chlamydomonas. Nitrogen deprivation, perhaps sensed as a drop in intracellular [NH4+], triggers a signal transduction pathway that results in altered gene expression and the induction of the gametogenic pathway. Blue light triggers a...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00016474
更新日期：1994-12-01 00:00:00
abstract：:The constituents of plasma membrane proteins, particularly the integral membrane proteins, are closely associated with the differentiation of plant cells. Secondary vascular differentiation, which gives rise to the increase in plant stem diameter, is the key process by which the volume of the plant body grows. However...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-011-9771-3
更新日期：2011-05-01 00:00:00
abstract：KEY MESSAGE:In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlyi...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-019-00876-0
更新日期：2019-07-01 00:00:00
abstract：:Protein folding in vivo is mediated by an array of proteins that act either as 'foldases' or 'molecular chaperones'. Foldases include protein disulfide isomerase and peptidyl prolyl isomerase, which catalyze the rearrangement of disulfide bonds or isomerization of peptide bonds around Pro residues, respectively. Molec...
journal_title：Plant molecular biology
pub_type： 杂志文章,评审
doi：10.1007/BF00039383
更新日期：1996-10-01 00:00:00
abstract：:Bacteroids of Rhizobium leguminosarum in root nodules of Pisum sativum are enclosed by a plant-derived peribacteriod membrane (PBM). The contents of the interstitial peribacteroid space (PBS) between bacteroid membrane and PBM were isolated by a controlled osmotic shock of PBM-enclosed bacteroids and analysed by two-d...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00015670
更新日期：1988-03-01 00:00:00
abstract：:Differential screening of cDNA libraries of unfertilized egg cells and in vitro zygotes of maize resulted in the isolation of more than 50 different genes whose expression is up- or down-regulated after in vitro fertilization (IVF). Among these genes, we identified a cDNA encoding the eukaryotic translation initiation...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/a:1006176819213
更新日期：1999-03-01 00:00:00
abstract：:The actin gene superfamily of Petunia hybrida cv. Mitchell contains greater than 100 gene members which have been divided into several highly divergent subfamilies [1]. Five subfamily-specific probes have been used to compare the actin genes among the Mitchell, Violet 23 (V23) and Red 51 (R51) cultivars of P. hybrida....
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00017466
更新日期：1988-09-01 00:00:00
abstract：:A homologue of the multidrug resistance (MDR) gene was obtained while screening a potato stolon tip cDNA expression library with 35S-labeled calmodulin. The mammalian MDR gene codes for a membrane-bound P-glycoprotein (170-180 kDa) which imparts multidrug resistance to cancerous cells. The potato cDNA (PMDR1) codes fo...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00042240
更新日期：1996-06-01 00:00:00
abstract：:We have sequenced and characterized the complete psbB gene cluster of Chlamydomonas reinhardtii chloroplast DNA. Although the petB and petD genes are located elsewhere, the sequential order of psbB, ORF31, psbN and psbH is identical to that of the psbB operon in higher plants. Also, intergenic non-coding regions are m...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00047405
更新日期：1993-07-01 00:00:00
abstract：:A mutant exhibiting conditional male sterility, in which fertility was restored under conditions of high humidity, was identified in T-DNA tagged lines of Arabidopsis thaliana. Scanning electron microscopy (SEM) demonstrated that the pollen surface was almost smooth and the reticulate pattern not prominent. Thus, the ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1023/B:PLAN.0000009269.97773.70
更新日期：2003-09-01 00:00:00
abstract：:The pea lectin (Psl) gene encodes an abundant seed protein. Its seed-specific expression pattern is conserved in transgenic tobacco plants. Progressive 5' promoter deletions resulted in a gradual decrease of transcriptional activity in tobacco seed. A fragment of 115 bp still conferred seed-specific expression albeit ...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/BF00019103
更新日期：1996-11-01 00:00:00
abstract：:We have previously shown that the expression of SmCP which encodes Solanum melongena cysteine proteinase is ethylene-inducible and is under circadian control. To understand the regulation of SmCP, a 1.34-kb SmCP 5'-flanking region and its deletion derivatives were analyzed for cis-elements using GUS and luc fusions an...
journal_title：Plant molecular biology
pub_type： 杂志文章
doi：10.1007/s11103-005-0954-7
更新日期：2005-03-01 00:00:00
